Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Jason D Limberis"'
Publikováno v:
PLoS ONE, Vol 18, Iss 11, p e0289442 (2023)
Non-polyadenylated RNA includes a large subset of crucial regulators of RNA expression and constitutes a substantial portion of the transcriptome, playing essential roles in gene regulation. For example, enhancer RNAs are long non-coding RNAs that pe
Externí odkaz:
https://doaj.org/article/f56ffaac10e1489b830d881f08480ce0
Publikováno v:
PLoS ONE, Vol 18, Iss 9, p e0288687 (2023)
Targeted amplicon sequencing to identify pathogens, resistance-conferring mutations, and strain types is an important tool in diagnosing and treating infections. However, due to the short read limitations of Illumina sequencing, many applications req
Externí odkaz:
https://doaj.org/article/7f7c3e72b95d4d5bb1ed0e3fb9f902a6
Autor:
Jason D Limberis
Ribosome profiling is a powerful technique used to study translation at a genome-wide level. It involves the sequencing of ribosome-protected mRNA fragments to determine the positions of ribosomes on transcripts. This information can be used to infer
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7d55a6f3b9f529d9372c53b7f45b469b
https://doi.org/10.17504/protocols.io.q26g7ykmqgwz/v1
https://doi.org/10.17504/protocols.io.q26g7ykmqgwz/v1
Circularization of rv0678 for Genotypic Bedaquiline Resistance Testing of Mycobacterium tuberculosis
Publikováno v:
Microbiology spectrum, vol 11, iss 2
Circular DNA offers benefits over linear DNA in diagnostic and field assays, but currently, circular DNA generation is lengthy, inefficient, highly dependent on the length and sequence of DNA, and can result in unwanted chimeras. We present streamlin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::75a8e7af19dcd759f83aff1a2477ac4c
https://escholarship.org/uc/item/42q5b9gs
https://escholarship.org/uc/item/42q5b9gs
Autor:
Jason D Limberis
The ligation of dumbbell (hairpin) oligos to linear dsDNA produces pseudo-circular DNA. Including deoxyUridine in the PCR primer sequences causes Q5 and other high-fidelity polymerases to arrest elongation. This results in overhangs that were success
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::caf5440862276647c64296cdcf390640
https://doi.org/10.17504/protocols.io.36wgqj9zyvk5/v1
https://doi.org/10.17504/protocols.io.36wgqj9zyvk5/v1
Autor:
Jason D Limberis
We designed primers with a tail sequence that forms a six-nucleotide hairpin at temperature
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::92c5a26f3e2f9c2bc8466ed0724dad1f
https://doi.org/10.17504/protocols.io.3byl4j24rlo5/v1
https://doi.org/10.17504/protocols.io.3byl4j24rlo5/v1
Autor:
Jason D. Limberis, John Z. Metcalfe
Publikováno v:
HardwareX, Vol 19, Iss , Pp e00576- (2024)
turboLysis is a novel mechanical cell lysis device that utilizes small beads to efficiently lyse tough cells like Mycobacterium, Saccharomyces, and Arabidopsis. We compared turboLysis to bead beating using the BeadBug 6 for several concentrations of
Externí odkaz:
https://doaj.org/article/8eb386147ac746f78073ea43b29db2e0
Autor:
Jason D. Limberis, John Z. Metcalfe
Publikováno v:
BMC Bioinformatics, Vol 24, Iss 1, Pp 1-8 (2023)
Abstract Background Multiplex PCR amplifies numerous targets in a single tube reaction and is essential in molecular biology and clinical diagnostics. One of its most important applications is in the targeted sequencing of pathogens. Despite this imp
Externí odkaz:
https://doaj.org/article/96debafe82894f4987a88c0719e5a714