Zobrazeno 1 - 10 of 21 pro vyhledávání: '"Jason C. Lin"'
1
Akademický článek
A deletion at the polled PC locus alone is not sufficient to cause a polled phenotype in cattle
Autor: Sadie L. Hennig, Joseph R. Owen, Jason C. Lin, Bret R. McNabb, Alison L. Van Eenennaam, James D. Murray
Publikováno v: Scientific Reports, Vol 12, Iss 1, Pp 1-12 (2022)
Abstract Dehorning is a common practice in the dairy industry, but raises animal welfare concerns. A naturally occurring genetic mutation (PC allele) comprised of a 212 bp duplicated DNA sequence replacing a 10-bp sequence at the polled locus is asso
Externí odkaz: https://doaj.org/article/56cc6fbf9a9146dea2eebc6fa2c27a02
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2
Akademický článek
One-step generation of a targeted knock-in calf using the CRISPR-Cas9 system in bovine zygotes
Autor: Joseph R. Owen, Sadie L. Hennig, Bret R. McNabb, Tamer A. Mansour, Justin M. Smith, Jason C. Lin, Amy E. Young, Josephine F. Trott, James D. Murray, Mary E. Delany, Pablo J. Ross, Alison L. Van Eenennaam
Publikováno v: BMC Genomics, Vol 22, Iss 1, Pp 1-11 (2021)
Abstract Background The homologous recombination (HR) pathway is largely inactive in early embryos prior to the first cell division, making it difficult to achieve targeted gene knock-ins. The homology-mediated end joining (HMEJ)-based strategy has b
Externí odkaz: https://doaj.org/article/8631b0fe8f854c6b809a56145bbc2a7d
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3
Akademický článek
Evaluation of mutation rates, mosaicism and off target mutations when injecting Cas9 mRNA or protein for genome editing of bovine embryos
Autor: Sadie L. Hennig, Joseph R. Owen, Jason C. Lin, Amy E. Young, Pablo J. Ross, Alison L. Van Eenennaam, James D. Murray
Publikováno v: Scientific Reports, Vol 10, Iss 1, Pp 1-9 (2020)
Abstract The CRISPR/Cas9 genome editing tool has the potential to improve the livestock breeding industry by allowing for the introduction of desirable traits. Although an efficient and targeted tool, the CRISPR/Cas9 system can have some drawbacks, i
Externí odkaz: https://doaj.org/article/a638503bf58644bba5b9de2748db09bb
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4
Akademický článek
Electroporation-Mediated Genome Editing of Livestock Zygotes
Autor: Jason C. Lin, Alison L. Van Eenennaam
Publikováno v: Frontiers in Genetics, Vol 12 (2021)
The introduction of genome editing reagents into mammalian zygotes has traditionally been accomplished by cytoplasmic or pronuclear microinjection. This time-consuming procedure requires expensive equipment and a high level of skill. Electroporation
Externí odkaz: https://doaj.org/article/3f674f1e00644c57b01f826568049a12
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6
Evaluation of mutation rates, mosaicism and off target mutations when injecting Cas9 mRNA or protein for genome editing of bovine embryos
Autor: Alison L. Van Eenennaam, Joseph R. Owen, Jason C. Lin, James D. Murray, Pablo J. Ross, Amy E. Young, Sadie L. Hennig
Publikováno v: Scientific Reports, Vol 10, Iss 1, Pp 1-9 (2020)
Scientific reports, vol 10, iss 1
Scientific Reports
The CRISPR/Cas9 genome editing tool has the potential to improve the livestock breeding industry by allowing for the introduction of desirable traits. Although an efficient and targeted tool, the CRISPR/Cas9 system can have some drawbacks, including
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a8ab7a969c2c91d1e2ac8dd4e9e74035
https://doaj.org/article/a638503bf58644bba5b9de2748db09bb
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7
Harnessing endogenous repair mechanisms for targeted gene knock-in of bovine embryos
Autor: Bret R. McNabb, Sadie L. Hennig, Jason C. Lin, Pablo J. Ross, James D. Murray, Alison L. Van Eenennaam, Amy E. Young, Joseph R. Owen
Publikováno v: Scientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
Scientific reports, vol 10, iss 1
Scientific Reports
Introducing useful traits into livestock breeding programs through gene knock-ins has proven challenging. Typically, targeted insertions have been performed in cell lines, followed by somatic cell nuclear transfer cloning, which can be inefficient. A
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c6347c52bf118ac4325bd5ba3cfa4879
http://link.springer.com/article/10.1038/s41598-020-72902-x
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9
A deletion at the polled P
Autor: Sadie L, Hennig, Joseph R, Owen, Jason C, Lin, Bret R, McNabb, Alison L, Van Eenennaam, James D, Murray
Publikováno v: Scientific reports. 12(1)
Dehorning is a common practice in the dairy industry, but raises animal welfare concerns. A naturally occurring genetic mutation (P
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::1585a524cfbc243e9fad34f74ad8c918
https://pubmed.ncbi.nlm.nih.gov/35136148
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10
One-step generation of a targeted knock-in calf using the CRISPR-Cas9 system in bovine zygotes
Autor: Pablo J. Ross, Sadie L. Hennig, Tamer A. Mansour, Josephine F. Trott, Bret R. McNabb, Jason C. Lin, Joseph R. Owen, Amy E. Young, Mary E. Delany, James D. Murray, Alison L. Van Eenennaam, Justin M. Smith
Publikováno v: BMC genomics, vol 22, iss 1
BMC Genomics
BMC Genomics, Vol 22, Iss 1, Pp 1-11 (2021)
Background The homologous recombination (HR) pathway is largely inactive in early embryos prior to the first cell division, making it difficult to achieve targeted gene knock-ins. The homology-mediated end joining (HMEJ)-based strategy has been shown
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1f6a8aeafcf523995c92a23095fa6d18
https://escholarship.org/uc/item/1gw9d6w6
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