Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Jason C Murphy"'
Publikováno v:
PLoS ONE, Vol 6, Iss 1, p e14512 (2011)
Immobilized Metal Affinity Chromatography (IMAC) has been used for decades to purify proteins on the basis of amino acid content, especially surface-exposed histidines and "histidine tags" genetically added to recombinant proteins. We and others have
Externí odkaz:
https://doaj.org/article/18d9747ef648442f897ad693030e06fb
Publikováno v:
Biotechnology Progress. 22:519-522
Mechanical lysis is an efficient and widely used method of liberating the contents of microbial cells, but the sensitivity of large nucleic acids to shear damage has prevented the application of mechanical lysis to DNA purification. It is demonstrate
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::60a99dd8aea16d82e71a58a85f5a2369
https://doi.org/10.1021/bp050422i
https://doi.org/10.1021/bp050422i
Autor:
Peilin Yu, Susan H. Hardin, Brent A. Mulder, Steve Anaya, Richard C. Willson, Keun Woo Lee, James M. Briggs, Jason C. Murphy, Anvy Nguyen, Xiaolian Gao
Publikováno v:
Nucleic Acids Research
The mechanism by which HIV-1 reverse transcriptase (HIV-RT) discriminates between the correct and incorrect nucleotide is not clearly understood. Chemically modified nucleotides containing 1-aminonaphthalene-5-sulfonate (ANS) attached to their gamma-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::139760ff57cc6c0b817f3305f1a70452
https://doi.org/10.1093/nar/gki779
https://doi.org/10.1093/nar/gki779
Publikováno v:
Enzyme and Microbial Technology. 33:865-883
The past several years have witnessed a rapidly increasing number of reports on utilizing plasmid DNA as a vector for the introduction of genes into mammalian cells for use in both gene therapy and vaccine applications. “Naked DNA vaccines” allow
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0c6f5f26942d83f2dde7315c4386db54
https://doi.org/10.1016/s0141-0229(03)00205-9
https://doi.org/10.1016/s0141-0229(03)00205-9
Publikováno v:
Protein Expression and Purification. 28:220-223
Recombinant proteins are often purified from microbial lysates containing high concentrations of nucleic acids. Pre-purification steps such as nuclease addition or precipitation with polyethyleneimine or ammonium sulfate are normally required to redu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6aaf2068fefecc6caf352039aacecc88
https://doi.org/10.1016/s1046-5928(02)00677-0
https://doi.org/10.1016/s1046-5928(02)00677-0
Publikováno v:
Journal of Immunological Methods. 274:257-264
Plasmid DNA inoculations can induce both humoral and cellular immunity, and this technique is now being employed in developing vaccination regimens for a large number of applications. DNA vaccination studies require the preparation of large amounts o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e18fe841b08b5c1a7fd72a85b6c6feae
https://doi.org/10.1016/s0022-1759(02)00516-1
https://doi.org/10.1016/s0022-1759(02)00516-1
Publikováno v:
Journal of Chromatography A. 984:215-221
The use of adsorptive chromatography for preparative nucleic acid separations is often limited by low capacity. The possibility that the adsorbent surface area sterically accessible to nucleic acid molecules could be increased by reducing their radiu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bbf510031edf8f999f10cd24b932c57b
https://doi.org/10.1016/s0021-9673(02)01814-9
https://doi.org/10.1016/s0021-9673(02)01814-9
Autor:
George E. Fox, Sindhu Balan, Igor Yu. Galaev, Richard C. Willson, Jason C. Murphy, Ashok Kumar, Bo Mattiasson
Publikováno v:
Biotechnology Letters. 25:1111-1116
The affinity of metal chelates for amino acids, such as histidine, is widely used in purifying proteins, most notably through six-histidine 'tails'. We have found that metal affinity interactions can also be applied to separation of single-stranded n
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8274feff0a2c1d5f8d7bcaa55f081a80
https://doi.org/10.1023/a:1024148316322
https://doi.org/10.1023/a:1024148316322
Autor:
Mary E. Dickinson, Sherry M. Haynes, Richard C. Willson, Jason C. Murphy, Kenneth J. Longmuir, Alan J. Waring
Publikováno v:
Molecular Therapy. 4(1):66-74
Here we report the characterization and optimization of a peptide/non-cationic lipid gene delivery system that successfully produces high levels of gene expression when delivered by microinjection into chicken embryos in vivo. In addition to plasmid
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a459e0363f00eaccdd9a42a26d19b102
Publikováno v:
PLoS ONE, Vol 6, Iss 1, p e14512 (2011)
PLoS ONE
PLoS ONE
Immobilized Metal Affinity Chromatography (IMAC) has been used for decades to purify proteins on the basis of amino acid content, especially surface-exposed histidines and "histidine tags" genetically added to recombinant proteins. We and others have
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d6f40159a846ccf1d72812528327cb01
http://europepmc.org/articles/PMC3021510?pdf=render
http://europepmc.org/articles/PMC3021510?pdf=render