Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Jared L. Dopp"'
Publikováno v:
Synthetic and Systems Biotechnology, Vol 4, Iss 4, Pp 204-211 (2019)
Cell-free protein synthesis (CFPS) is an established biotechnology tool that has shown great utility in many applications such as prototyping proteins, building genetic circuits, designing biosensors, and expressing cytotoxic proteins. Although CFPS
Externí odkaz:
https://doaj.org/article/63ab850c9c1a497a8aa3de0e43be1c19
Autor:
Adam R. Carr, Jared L. Dopp, Kaiyue Wu, Peivand Sadat Mousavi, Yeong Ran Jo, Ciara E. McNeley, Zachary T. Lynch, Keith Pardee, Alexander A. Green, Nigel F. Reuel
Publikováno v:
ACS sensors. 7(3)
The COVID-19 pandemic has emphasized the importance of widespread testing to control the spread of infectious diseases. The rapid development, scale-up, and deployment of viral and antibody detection methods since the beginning of the pandemic have g
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc5d87e52dbe931176b206cb885b91c9
https://pubmed.ncbi.nlm.nih.gov/35254055
https://pubmed.ncbi.nlm.nih.gov/35254055
Publikováno v:
AIChE J
Optimal tip sonication settings, namely tip position, input power, and pulse durations, are necessary for temperature sensitive procedures like preparation of viable cell extract. In this paper, the optimum tip immersion depth (20–30% height below
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::551ded44668adb98f0aedc3e9cc01a17
https://europepmc.org/articles/PMC9161774/
https://europepmc.org/articles/PMC9161774/
Publikováno v:
Biotechnology and Bioengineering. 116:667-676
In this study, we present a minimal template design and accompanying methods to produce assayable quantities of custom sequence proteins within 24 hr from receipt of inexpensive gene fragments from a DNA synthesis vendor. This is done without the con
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::030dee37cbdf6d0aca2146a0d22749b8
https://doi.org/10.1002/bit.26912
https://doi.org/10.1002/bit.26912
Autor:
Nigel F. Reuel, Jared L. Dopp
Publikováno v:
J Vis Exp
This protocol describes the design of a minimal DNA template and the steps for enzymatic amplification, enabling rapid prototyping of assayable proteins in less than 24 h using cell-free expression. After receiving DNA from a vendor, the gene fragmen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ccb507a2e2418c594a84bc67d09bb4a
https://doi.org/10.3791/62728
https://doi.org/10.3791/62728
Publikováno v:
ACS Synth Biol
Cell free protein expression (CFPS) from E. coli cell lysate is an established chemical biology technique. Common efforts to improve synthesis capacity, such as strain engineering and process improvements, have overlooked the opportunity to increase
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0373c9be64136a629d2ac9a7b9bd4648
https://europepmc.org/articles/PMC8168642/
https://europepmc.org/articles/PMC8168642/
Publikováno v:
Bioanalysis. 13(5)
Aim: For oncolytic virus trials, regulatory agencies often require pharmaceutical industry to evaluate risks of released viruses from patients to environment. This study was to establish a real-time PCR method to assess viral shedding and viral stabi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7fdca65c7939ef4833a3b76ec5c1228c
https://pubmed.ncbi.nlm.nih.gov/33661025
https://pubmed.ncbi.nlm.nih.gov/33661025
Optimal tip sonication settings, namely tip position, input power, and pulse durations, are necessary to ensure proper mixing and maintain solution temperature below a critical temperature. This is significant for temperature sensitive procedures lik
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::cffc6a02548a064041d8d17f0370bcb3
https://doi.org/10.22541/au.161454367.72105176/v1
https://doi.org/10.22541/au.161454367.72105176/v1
Autor:
Nigel F. Reuel, Jared L. Dopp
Publikováno v:
Biochemical Engineering Journal. 138:21-28
Cell-free protein synthesis (CFPS) is a versatile tool for protein research and biomanufacturing. In this work, large volume cell extract preparation methods from Escherichia coli were optimized with a unit operations approach. Using face centered cu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d576d948a15465766574d3ee64cba13d
https://doi.org/10.1016/j.bej.2018.06.021
https://doi.org/10.1016/j.bej.2018.06.021
Simple, Functional, Inexpensive Cell Extract forin vitroPrototyping of Proteins with Disulfide Bonds
Autor:
Jared L. Dopp, Nigel F. Reuel
In vitroexpression of proteins fromE. coliextract is a useful method for prototyping and production of cytotoxic or unnatural products. However, proteins that have multiple disulfide bonds require custom extract that, to date, requires careful additi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b918b48e03c0daa49916a71966bdc858
https://doi.org/10.1101/2019.12.19.883413
https://doi.org/10.1101/2019.12.19.883413