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Functionally important residues of the Tet repressor inducing peptide TIP determined by a complete mutational analysis

Autor: Marcus Klotzsche, Christian Berens, Kaoutar Mehdaoui, Janko Daam, Klaus Pfleiderer, Wolfgang Hillen

Publikováno v: Gene. 423(2)

Tet repressor (TetR) is widely used to control gene expression in pro- and eukaryotes. The mechanism of induction by its natural inducer tetracycline is well characterized. A 16-mer oligopeptide, called TIP, fused to thioredoxin A (TrxA) of Escherich

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::25444af7694b9c54e3f51ada82ecd2e9
https://pubmed.ncbi.nlm.nih.gov/18672042

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Random insertion of a TetR-inducing peptide tag into Escherichia coli proteins allows analysis of protein levels by induction of reporter gene expression

Autor: Janko Daam, Wolfgang Hillen, Maximilian Schlicht, Christian Berens

Publikováno v: Applied and environmental microbiology. 72(8)

The insertion element InsTipα was constructed to generate protein expression data. It randomly fuses the TetR-inducing peptide Tip to the affected reading frame. Fusion protein expression is quantified by Tet-regulated reporter gene expression. The

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::23bc5a87f5b267efde3852c557f307fe
https://pubmed.ncbi.nlm.nih.gov/16885321

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