A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium

Autor: Franka H. van der Linden, Eike K. Mahlandt, Janine J. G. Arts, Joep Beumer, Jens Puschhof, Saskia M. A. de Man, Anna O. Chertkova, Bas Ponsioen, Hans Clevers, Jaap D. van Buul, Marten Postma, Theodorus W. J. Gadella, Joachim Goedhart

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)

Currently, genetically encoded calcium indicators are not suitable for direct quantification. Here the authors engineer a fluorescence lifetime imaging calcium biosensor, Turquoise Calcium Fluorescence LIfeTime Sensor (Tq-Ca-FLITS), and measure intra

Externí odkaz: https://doaj.org/article/28e02e090b82435fa3bc07ed281bc827

Intercellular adhesion molecule 2 regulates diapedesis hotspots by allowing neutrophil crawling against the direction of flow

Autor: Max L B Grönloh, Merel E Tebbens, Marianthi Kotsi, Janine J G Arts, Jaap D van Buul

Publikováno v: Vascular Biology, Vol 5, Iss 1, Pp 1-13 (2023)

Intercellular adhesion molecules (ICAMs) are cell surface proteins that play a crucial role in the body’s immune response and inflammatory processes. ICAM1 and ICAM2 are two ICAM family members expressed on the surface of various cell types, includ

Externí odkaz: https://doaj.org/article/4f15ddf2758745e1b658499b089f0162

Endothelial transmigration hotspots limit vascular leakage through heterogeneous expression of ICAM-1

Autor: Max L B Grönloh, Janine J G Arts, Sebastián Palacios Martínez, Amerens A van der Veen, Lanette Kempers, Abraham C I van Steen, Joris J T H Roelofs, Martijn A Nolte, Joachim Goedhart, Jaap D van Buul

Publikováno v: Grönloh, M L B, Arts, J J G, Palacios Martínez, S, van der Veen, A A, Kempers, L, van Steen, A C I, Roelofs, J J T H, Nolte, M A, Goedhart, J & van Buul, J D 2023, ' Endothelial transmigration hotspots limit vascular leakage through heterogeneous expression of ICAM-1 ', EMBO Reports, vol. 24, no. 1, e55483 . https://doi.org/10.15252/embr.202255483
EMBO reports, 24(1):e55483. Wiley-Blackwell
EMBO Reports, 24(1):e55483. Wiley-Blackwell

Upon inflammation, leukocytes leave the circulation by crossing the endothelial monolayer at specific transmigration ‘hotspot’ regions. Although these regions support leukocyte transmigration, their functionality is not clear. We found that endot

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::668e948a7eee2654f8daa2c0cb6818aa
https://research.vumc.nl/en/publications/977802a3-56bf-40d5-8031-092edf437de6

Visualizing endogenous Rho activity with an improved localization-based, genetically encoded biosensor

Autor: Werner J. van der Meer, Simon Tol, Eike K. Mahlandt, Jaap D. van Buul, Franka H van der Linden, Theodorus W. J. Gadella, Janine J. G. Arts, Joachim Goedhart

Publikováno v: Journal of cell science, 134(17):jcs258823.. Company of Biologists Ltd
Journal of Cell Science, 134(17):258823. Company of Biologists Ltd
Journal of Cell Science
article-version (VoR) Version of Record

Rho GTPases are regulatory proteins, which orchestrate cell features such as morphology, polarity and movement. Therefore, probing Rho GTPase activity is key to understanding processes such as development and cell migration. Localization-based report

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2562c53b256ee5a71a0532038caed7ff
https://pure.amc.nl/en/publications/visualizing-endogenous-rho-activity-with-an-improved-localizationbased-genetically-encoded-biosensor(5ebcbd54-fbcc-45ee-8f6c-da6e3ec0d3a8).html

Endothelial junctional membrane protrusions serve as hotspots for neutrophil transmigration

Autor: Simon Tol, John M. Heddleston, Barbara Walzog, Eric Wait, Ivar Noordstra, Eloi Montanez, Marten Postma, Abraham C.I. van Steen, Joachim Goedhart, Lilian Schimmel, Martijn A. Nolte, Teng-Leong Chew, Mark R. Winter, Emma Gordon, Eike K. Mahlandt, Jos van Rijssel, Max L. B. Grönloh, Janine J. G. Arts, Jaap D. van Buul, Satya Khuon

Publikováno v: eLife, Vol 10 (2021)
eLife, 10:e66074. eLife Sciences Publications
Dipòsit Digital de la UB
Universidad de Barcelona
eLife

Upon inflammation, leukocytes rapidly transmigrate across the endothelium to enter the inflamed tissue. Evidence accumulates that leukocytes use preferred exit sites, alhough it is not yet clear how these hotspots in the endothelium are defined and h

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ec16b5310098b2e2e9c9865107ced55
https://elifesciences.org/articles/66074