Zobrazeno 1 - 5
of 5
pro vyhledávání: '"Jane Therese Mooney"'
Autor:
Milton Thomas William Hearn, Jane Therese Mooney, Dale Patricia Fredericks, Christine Brunn Schiodt, Thorkild Christensen
Publikováno v:
Journal of Molecular Recognition. 28:401-412
The ability of a new class of metal binding tags to facilitate the purification of recombinant proteins, exemplified by the tagged glutathione S-transferase and human growth hormone, from Escherichia coli fermentation broths and lysates has been furt
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::469b3f34f690f56e561ad502b10ddfb8
https://doi.org/10.1002/jmr.2456
https://doi.org/10.1002/jmr.2456
Autor:
Dale Patricia Fredericks, Milton Thomas William Hearn, Jane Therese Mooney, Thorkild Christensen
Publikováno v:
Biotechnology Journal. 9:1023-1032
The complete enzymatic removal of affinity tags from tagged recombinant proteins is often required but can be challenging when slow points for cleavage exist. This study documents a general approach to remove N-terminal tags from recombinant proteins
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::21f057c7f12ecc3c66f0ebed367cb62b
https://doi.org/10.1002/biot.201300546
https://doi.org/10.1002/biot.201300546
Publikováno v:
Separation and Purification Technology. 120:265-274
This study documents the use of an IMAC cassette for the purification of N-terminally tagged recombinant proteins. The procedures are based on the application of the immobilised macrocyclic ligand, 1,4,7-triazacyclononance (tacn), with a set of new t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::61db7d4a0b6aa63632230c9cf9ae5a75
https://doi.org/10.1016/j.seppur.2013.09.045
https://doi.org/10.1016/j.seppur.2013.09.045
Autor:
Chunfang Zhang, Christine Bruun Schiødt, Milton Thomas William Hearn, Jane Therese Mooney, Christina Jespergaard, Dale Patricia Fredericks, Thorkild Christensen
Publikováno v:
Protein expression and purification. 94
In this study, integration of three discrete process aspects of the IMAC purification of Escherichia coli expressed recombinant proteins has been investigated. To this end, novel N-terminally tagged human growth hormone variants (tagged-vhGHs) have b
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e571025ed150c3ce905573e784bf58f
https://pubmed.ncbi.nlm.nih.gov/24275639
https://pubmed.ncbi.nlm.nih.gov/24275639
Publikováno v:
Journal of chromatography. A. 1218(1)
This study describes the screening of a peptide phage display library for amino acid sequences that bind with different affinities to a novel class of chelating ligands complexed with Ni²+ ions. These chelating ligands are based on the 1,4,7-triazac
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fe49e19d83e35d7a6e2afa6137f08ae9
https://pubmed.ncbi.nlm.nih.gov/21159343
https://pubmed.ncbi.nlm.nih.gov/21159343