Novel kinetoplastid-specific cAMP binding proteins identified by RNAi screening for cAMP resistance in Trypanosoma brucei

Autor: Sabine Bachmaier, Matthew K. Gould, Eleni Polatoglou, Radoslaw Omelianczyk, Ana E. Brennand, Maha A. Aloraini, Jane C. Munday, David Horn, Michael Boshart, Harry P. de Koning

Publikováno v: Frontiers in Cellular and Infection Microbiology, Vol 13 (2023)

Cyclic AMP signalling in trypanosomes differs from most eukaryotes due to absence of known cAMP effectors and cAMP independence of PKA. We have previously identified four genes from a genome-wide RNAi screen for resistance to the cAMP phosphodiestera

Externí odkaz: https://doaj.org/article/a23c0cd921084cd7b7799b4ed7628644

Chimerization at the AQP2–AQP3 locus is the genetic basis of melarsoprol–pentamidine cross-resistance in clinical Trypanosoma brucei gambiense isolates

Autor: Fabrice E. Graf, Nicola Baker, Jane C. Munday, Harry P. de Koning, David Horn, Pascal Mäser

Publikováno v: International Journal for Parasitology: Drugs and Drug Resistance, Vol 5, Iss 2, Pp 65-68 (2015)

Aquaglyceroporin-2 is a known determinant of melarsoprol–pentamidine cross-resistance in Trypanosoma brucei brucei laboratory strains. Recently, chimerization at the AQP2–AQP3 tandem locus was described from melarsoprol–pentamidine cross-resist

Externí odkaz: https://doaj.org/article/20954e2ab8b0455dba5be27f96e76043

Cloning and functional complementation of ten Schistosoma mansoni phosphodiesterases expressed in the mammalian host stages.

Autor: Jane C Munday, Stefan Kunz, Titilola D Kalejaiye, Marco Siderius, Susanne Schroeder, Daniel Paape, Ali H Alghamdi, Zainab Abbasi, Sheng Xiang Huang, Anne-Marie Donachie, Samia William, Abdel Nasser Sabra, Geert Jan Sterk, Sanaa S Botros, David G Brown, Charles S Hoffman, Rob Leurs, Harry P de Koning

Publikováno v: PLoS Neglected Tropical Diseases, Vol 14, Iss 7, p e0008447 (2020)

Only a single drug against schistosomiasis is currently available and new drug development is urgently required but very few drug targets have been validated and characterised. However, regulatory systems including cyclic nucleotide metabolism are em

Externí odkaz: https://doaj.org/article/dec44c4ba15a44afbe2fcc56f7a87eca

Screening of a PDE-focused library identifies imidazoles with in vitro and in vivo antischistosomal activity

Autor: Irene G. Salado, Naglaa M. El-Lakkany, Maarten Sijm, Sayed H. Seif el-Din, Carmen Gil, Abdel-Nasser A. Sabra, Erik de Heuvel, Harry P. de Koning, A.R. Blaazer, Jane C. Munday, Yang Zheng, Alfonso García-Rubia, Louis Maes, Sanaa S. Botros, Iwan J. P. de Esch, Samia William, Geert Jan Sterk, Rob Leurs, Koen Augustyns, Víctor Sebastián-Pérez

Publikováno v: International Journal for Parasitology: Drugs and Drug Resistance
Digital.CSIC. Repositorio Institucional del CSIC
instname
Botros, S S, William, S, Sabra, A N A, El-Lakkany, N M, Seif el-Din, S H, García-Rubia, A, Sebastián-Pérez, V, Blaazer, A R, de Heuvel, E, Sijm, M, Zheng, Y, Salado, I G, Munday, J C, Maes, L, de Esch, I J P, Sterk, G J, Augustyns, K, Leurs, R, Gil, C & De Koning, H P 2019, ' Screening of a PDE-focused library identifies imidazoles with in vitro and in vivo antischistosomal activity ', International Journal for Parasitology: Drugs and Drug Resistance, vol. 9, pp. 35-43 . https://doi.org/10.1016/j.ijpddr.2019.01.001
International Journal for Parasitology: Drugs and Drug Resistance, Vol 9, Iss, Pp 35-43 (2019)
International Journal for Parasitology: Drugs and Drug Resistance, 9, 35-43. Elsevier

9 p.-5 fig.-2 tab.
We report the evaluation of 265 compounds from a PDE-focused library for their antischistosomal activity, assessed in vitro using Schistosoma mansoni. Of the tested compounds, 171 (64%) displayed selective in vitro activity, w

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::91c92e19004a518cecbe3412299fb621
https://doi.org/10.1016/j.ijpddr.2019.01.001

Author response: Positively selected modifications in the pore of TbAQP2 allow pentamidine to enter Trypanosoma brucei

Autor: Anna Dimitriou, Daniel Paape, Christopher M Woodley, Ulrich Zachariae, Anthonius A. Eze, Simone Weyand, Ibrahim A. Teka, Ali H. Alghamdi, Richard R. Tidwell, Arvind Kumar, Jane C. Munday, Juan F. Quintana, Mark Carrington, Mohammed I. Al-Salabi, Paul M. O'Neill, Laura F Anderson, Maria Esther Martin Abril, Harry P. de Koning, Teresa Sprenger, Hasan M. S. Ibrahim, Fredrik Svensson, Simon Gudin, Fabian Hulpia, Patrik Milic, Chinyere E Okpara, Gustavo D. Campagnaro, Dominik Gurvic, Graeme Smart, David W. Boykin, Christophe Dardonville, Luca Settimo, Siu Pui Ying Kelly, Laura Watson, Joanna Wielinska, Mark C. Field

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::fa648917f3111794e8be1fa4dd747110
https://doi.org/10.7554/elife.56416.sa2