Enabling comprehensive optogenetic studies of mouse hearts by simultaneous opto-electrical panoramic mapping and stimulation

Autor: Michael Rieger, Christian Dellenbach, Johannes vom Berg, Jane Beil-Wagner, Ange Maguy, Stephan Rohr

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)

Current cardiac mapping systems provide either electrical or optical readouts. Here the authors report a panoramic opto-electrical measurement and stimulation (POEMS) system which embraces the entire ventricular surface of mouse hearts, allowing flex

Externí odkaz: https://doaj.org/article/3b4291aa43f942efab0f15dab214ad98

Enabling comprehensive optogenetic studies of mouse hearts by simultaneous opto-electrical panoramic mapping and stimulation

Autor: Stephan Rohr, Jane Beil-Wagner, Johannes vom Berg, Michael Rieger, Ange Maguy, Christian Dellenbach

Publikováno v: Rieger, Michael; Dellenbach, Christian; vom Berg, Johannes; Beil-Wagner, Jane; Maguy, Ange; Rohr, Stephan (2021). Enabling comprehensive optogenetic studies of mouse hearts by simultaneous opto-electrical panoramic mapping and stimulation. Nature communications, 12(1), p. 5804. Nature Publishing Group 10.1038/s41467-021-26039-8
Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
Nature Communications

During the last decade, cardiac optogenetics has turned into an essential tool for investigating cardiac function in general and for assessing functional interactions between different myocardial cell types in particular. To advance exploitation of t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ac612f587b9d03e263f2f68767e33a71
https://boris.unibe.ch/159884/1/Rieger2021Enablingco.pdf

T cell-specific inactivation of mouse CD2 by CRISPR/Cas9

Autor: Markus Gerhard, Thorsten Buch, Florian Mair, Martina Grandl, Georg Dössinger, Burkhard Becher, Jane Beil-Wagner, Kilian Schober, Dirk H. Busch, Achim Tresch, Pushpalatha Palle, Johannes vom Berg

Publikováno v: Scientific reports
Scientific Reports

The CRISPR/Cas9 system can be used to mutate target sequences by introduction of double-strand breaks followed by imprecise repair. To test its use for conditional gene editing we generated mice transgenic for CD4 promoter-driven Cas9 combined with g

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a4ea477caff49a826e9ba08443e5aea4
https://doi.org/10.1038/srep21377

Generation of Bacterial Artificial Chromosome (BAC) Transgenic Mice

Autor: Thorsten Buch, Jane Beil

Publikováno v: Methods in Molecular Biology ISBN: 9781493912148

Transgenic mice are among the most helpful tools to study the role of genes in physiological conditions. In this protocol, we describe the generation of bacterial artificial chromosome (BACs) constructs, which are used to express a gene of interest u

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e3a5f4bad4385384481624ffc0de86e4
https://doi.org/10.1007/978-1-4939-1215-5_8

Is BAC Transgenesis Obsolete? State of the Art in the Era of Designer Nucleases

Autor: Thorsten Buch, Jane Beil, Pawel Pelczar, Lynsey Fairbairn

Publikováno v: Journal of Biomedicine and Biotechnology
Journal of Biomedicine and Biotechnology, Vol 2012 (2012)

DNA constructs based on bacterial artificial chromosomes (BACs) are frequently used to generate transgenic animals as they reduce the influence of position effects and allow predictable expression patterns for genes whose regulatory sequences are not

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::141652497dc689b41d6e807ca9cb5f9e