Efficient Synthesis of DNA Duplexes Containing Reduced Acetaldehyde Interstrand Cross-Links

Autor: Sally B. Morton, L. David Finger, Roxanne van der Sluijs, William D. Mulcrone, Michael Hodskinson, Christopher L. Millington, Christina Vanhinsbergh, Ketan J. Patel, Mark J. Dickman, Puck Knipscheer, Jane A. Grasby, David M. Williams

Publikováno v: Journal of the American Chemical Society.

DNA interstrand cross-links (ICLs) prevent DNA replication and transcription and can lead to potentially lethal events, such as cancer or bone marrow failure. ICLs are typically repaired by proteins within the Fanconi Anemia (FA) pathway, although th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd1b46b5a757461d1e3eaa694eafae56
https://pubmed.ncbi.nlm.nih.gov/36584283

Regional conformational flexibility couples substrate specificity and scissile phosphate diester selectivity in human flap endonuclease 1

Autor: Jack C. Exell, Jane A. Grasby, Andrea M. Hounslow, Nicola J. Baxter, Ian A. Bennet, L. David Finger, Mark J. Thompson, Nur Nazihah B Md Shahari, Benjamin Ambrose, Timothy D. Craggs, Jonathan P. Waltho

Publikováno v: Nucleic Acids Research

Human flap endonuclease-1 (hFEN1) catalyzes the divalent metal ion-dependent removal of single-stranded DNA protrusions known as flaps during DNA replication and repair. Substrate selectivity involves passage of the 5′-terminus/flap through the arc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::880866fe59c777b697bae06a26fe7690
http://europepmc.org/articles/PMC6009646

Cellular Active N-Hydroxyurea FEN1 Inhibitors Block Substrate Entry to the Active Site

Autor: Stephen T. Durant, L. David Finger, Mark J. Thompson, Jane A. Grasby, Thomas Ward, J. Willem M. Nissink, W. Mark Abbott, Steven J. Shaw, Catrine Sioberg, Clifford David Jones, Judit E. Debreczeni, Jack C. Exell, Claire McWhirter, Daniel Martinez Molina

Publikováno v: Nature chemical biology

The structure-specific nuclease human flap endonuclease-1 (hFEN1) plays a key role in DNA replication and repair and may be of interest as an oncology target. We present the crystal structure of inhibitor-bound hFEN1, which shows a cyclic N-hydroxyur

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::acad248f883063128e76237c1086c207
http://europepmc.org/articles/PMC5348030

Phosphate steering by Flap Endonuclease 1 promotes 5′-flap specificity and incision to prevent genome instability

Autor: Steven J. Shaw, Alexander J. Neil, Fahad Rashid, Altaf H. Sarker, Mark J. Thompson, Jane A. Grasby, Mai Zong Her, Victoria J. B. Gotham, Susan E. Tsutakawa, Emma C. Jardine, Andrew S. Arvai, Samir M. Hamdan, John A. Tainer, Sergei M. Mirkin, L. David Finger, Sana I. Algasaier, Jane C. Kim

Publikováno v: Tsutakawa, SE; Thompson, MJ; Arvai, AS; Neil, AJ; Shaw, SJ; Algasaier, SI; et al.(2017). Phosphate steering by Flap Endonuclease 1 promotes 5′-flap specificity and incision to prevent genome instability. Nature Communications, 8. doi: 10.1038/ncomms15855. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/5zc3s29h
Nature communications, vol 8, iss 1
Nature Communications, Vol 8, Iss 1, Pp 1-15 (2017)
Nature Communications

DNA replication and repair enzyme Flap Endonuclease 1 (FEN1) is vital for genome integrity, and FEN1 mutations arise in multiple cancers. FEN1 precisely cleaves single-stranded (ss) 5′-flaps one nucleotide into duplex (ds) DNA. Yet, how FEN1 select

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::11333b994a0137c384e97a1b59040a0f
http://www.escholarship.org/uc/item/5zc3s29h

Alkyltransferase-like protein (Atl1) distinguishes alkylated guanines for DNA repair using cation–π interactions

Autor: Julie L. Tubbs, Geoffrey P. Margison, Bernard A. Connolly, Hannah Blackburn, Christopher L. Millington, Mary R Thorncroft, David M. Williams, Oliver J. Wilkinson, Christopher A. Hunter, John A. Tainer, Andrew S. Marriott, Rihito Morita, Gail McGown, Ryoji Masui, Jane A. Grasby, Vitaly F. Latypov, Amanda J. Watson

Publikováno v: Proceedings of the National Academy of Sciences. 109:18755-18760

Alkyltransferase-like (ATL) proteins in Schizosaccharomyces pombe (Atl1) and Thermus thermophilus (TTHA1564) protect against the adverse effects of DNA alkylation damage by flagging O 6 -alkylguanine lesions for nucleotide excision repair (NER). We s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aefb71f80de8a217758a25dc55b8ef72
https://doi.org/10.1073/pnas.1209451109