Zobrazeno 1 - 10
of 25
pro vyhledávání: '"Jana Lauzon"'
Autor:
Dammika P. Manage, Jana Lauzon, Christina M. Jones, Patrick J. Ward, Linda M. Pilarski, Patrick M. Pilarski, Lynn M. McMullen
Publikováno v:
BMC Microbiology, Vol 19, Iss 1, Pp 1-11 (2019)
Abstract Background Over a one year period, swabs of 820 beef carcasses were tested for the presence of Shiga toxin-producing Escherichia coli by performing Polymerase Chain Reaction (PCR) in a novel technology termed “cassette PCR”, in compariso
Externí odkaz:
https://doaj.org/article/7e408ecd19fc4fe3bd886ce883e8fe4f
Publikováno v:
BMC Microbiology, Vol 19, Iss 1, Pp 1-11 (2019)
Abstract Background Fast molecular detection methods benefit from ready-to-run lab-on-a-chip molecular assays with minimum preparation time. Detection efficiency of such methods can improve if multiple targets are detected simultaneously per given re
Externí odkaz:
https://doaj.org/article/f4688d359d9c4a48b2720dfe2bb3240f
Publikováno v:
Micromachines, Vol 12, Iss 8, p 959 (2021)
Detection sensitivity of cassette PCR was compared with a commercial BAX® PCR system for detection of eae and stx genes in Escherichia coli from 806 beef carcass swabs. Cassette PCR detects multiple genetic markers on multiple samples using PCR and
Externí odkaz:
https://doaj.org/article/ba0b9d8147864164ab32d866c45a4f5c
Autor:
Darin Hunt, Curtis Figley, Dammika P Manage, Jana Lauzon, Rachel Figley, Linda M Pilarski, Lynn M McMullen, Patrick M Pilarski
Publikováno v:
PLoS ONE, Vol 13, Iss 5, p e0197100 (2018)
In this manuscript, we report the design and development of a fast, reliable instrument to run gel-based cassette polymerase chain reactions (PCR). Here termed the GelCycler Mark II, our instrument is a miniaturized molecular testing system that is f
Externí odkaz:
https://doaj.org/article/62c1883695aa4d26b0339e6c0ec643c2
Publikováno v:
Micromachines
Volume 12
Issue 8
Micromachines, Vol 12, Iss 959, p 959 (2021)
Volume 12
Issue 8
Micromachines, Vol 12, Iss 959, p 959 (2021)
Detection sensitivity of cassette PCR was compared with a commercial BAX® PCR system for detection of eae and stx genes in Escherichia coli from 806 beef carcass swabs. Cassette PCR detects multiple genetic markers on multiple samples using PCR and
Autor:
Linda M. Pilarski, Jana Lauzon, Patrick Ward, Lynn M. McMullen, Christina M. Jones, Dammika P. Manage, Patrick M. Pilarski
Publikováno v:
BMC Microbiology, Vol 19, Iss 1, Pp 1-11 (2019)
BMC Microbiology
BMC Microbiology
Background Over a one year period, swabs of 820 beef carcasses were tested for the presence of Shiga toxin-producing Escherichia coli by performing Polymerase Chain Reaction (PCR) in a novel technology termed “cassette PCR”, in comparison to conv
Publikováno v:
BMC Microbiology, Vol 19, Iss 1, Pp 1-11 (2019)
BMC Microbiology
BMC Microbiology
Background Fast molecular detection methods benefit from ready-to-run lab-on-a-chip molecular assays with minimum preparation time. Detection efficiency of such methods can improve if multiple targets are detected simultaneously per given reaction. D
Autor:
Patrick M. Pilarski, Curtis Figley, Rachel Figley, Jana Lauzon, Linda M. Pilarski, Dammika P. Manage, Darin Hunt, Lynn M. McMullen
Publikováno v:
PLoS ONE, Vol 13, Iss 5, p e0197100 (2018)
PLoS ONE
PLoS ONE
In this manuscript, we report the design and development of a fast, reliable instrument to run gel-based cassette polymerase chain reactions (PCR). Here termed the GelCycler Mark II, our instrument is a miniaturized molecular testing system that is f
Autor:
R.W. Johnstone, Linda M. Pilarski, H. John Crabtree, Yuen Morrissey, Alexey Atrazhev, Christopher J. Backhouse, Jana Lauzon, Alexander J. Stickel, Brian J. Taylor, Dammika P. Manage, Tina Liang
Publikováno v:
Microfluidics and Nanofluidics. 13:383-398
In the course of developing a microfluidic analytical platform incorporating the polymerase chain reaction (PCR) and subsequent capillary electrophoresis (CE) analysis for a variety of bio-assays, we examined PCR inhibition through surface interactio
Autor:
Alexander J. Stickel, Jason P. Acker, Alexey Atrazhev, Dammika P. Manage, Linda M. Pilarski, Jana Lauzon, Yuen C. Morrissey
Publikováno v:
Microfluidics and Nanofluidics. 10:697-702
Performing medical diagnosis in microfluidic devices could scale down laboratory functions and reduce the cost for accessible healthcare. The ultimate goal of such devices is to receive a sample of blood, perform genetic amplification (polymerase cha