Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Jana Kopečná"'
Publikováno v:
Bio-Protocol, Vol 5, Iss 3 (2015)
Intermediates of tetrapyrrole biosynthetic pathway are low-abundant compounds, and their quantification is usually difficult, time consuming and requires large amounts of input material. Here, we describe a method allowing fast and accurate quantific
Externí odkaz:
https://doaj.org/article/844b2bfb8f4945418d43df9c653f0c16
Publikováno v:
Bio-Protocol, Vol 2, Iss 16 (2012)
This is an accurate method to assess the rate of chlorophyll biosynthesis in vivo in cyanobacteria. Given that labeled glutamate is used as the very early precursor of chlorophyll together with a short pulse of labeling (30 min), this method provides
Externí odkaz:
https://doaj.org/article/78c6294d8d4b44edbb044e086871fe78
Autor:
Roman Sobotka, Daniel P. Canniffe, Philip J. Jackson, Paul A. Davison, Jana Kopečná, Sarah Hollingshead, Mark J. Dickman, C. Neil Hunter
The cyclase step in chlorophyll (Chl) biosynthesis has not been characterized biochemically, although there are some plausible candidates for cyclase subunits. Two of these, Sll1214 and Sll1874 from the cyanobacterium Synechocystis 6803, were FLAG-ta
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aed076c0dd751290e38084f0be83c5b4
https://doi.org/10.1074/jbc.m112.352526
https://doi.org/10.1074/jbc.m112.352526
Autor:
Mihály Kis, Jana Kopečná, Aleš Tomčala, Roman Sobotka, Vendula Krynická, Zoltan Gombos, Jan Pilný, Josef Komenda
Publikováno v:
Plant Physiology. 169:1307-1317
The negatively charged lipid phosphatidylglycerol (PG) constitutes up to 10% of total lipids in photosynthetic membranes, and its deprivation in cyanobacteria is accompanied by chlorophyll (Chl) depletion. Indeed, radioactive labeling of the PG-deple
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::194c3ec44997437f425d27aeffeffbcc
https://doi.org/10.1104/pp.15.01150
https://doi.org/10.1104/pp.15.01150
Publikováno v:
Frontiers in Plant Science
Cyanobacterium Synechocystis PCC 6803 represents a favored model organism for photosynthetic studies. Its easy transformability allowed construction of a vast number of Synechocystis mutants including many photosynthetically incompetent ones. However
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::414f4b33bbf32da13f2e11285bd7d70d
https://pubmed.ncbi.nlm.nih.gov/27242849
https://pubmed.ncbi.nlm.nih.gov/27242849
Autor:
Lukáš Trantírek, Jana Kopečná, Alena Zíková, Julius Lukeš, Maria A. Schumacher, Kenneth Stuart
Publikováno v:
International Journal for Parasitology. 38:901-912
The mitochondrial RNA-binding proteins (MRP) 1 and 2 play a regulatory role in RNA editing and putative role(s) in RNA processing in Trypanosoma brucei. Here, we report the purification of a high molecular weight protein complex consisting solely of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bca5abcbb30280ac424a4ad2d5ccfc0a
https://doi.org/10.1016/j.ijpara.2007.12.009
https://doi.org/10.1016/j.ijpara.2007.12.009
Autor:
Roman Sobotka, Eliška Trsková, Eva Kotabová, Erica Belgio, Alexander V. Ruban, Jana Kopečná, Radek Kaňa
Publikováno v:
FEBS letters. 590(8)
Non-photochemical quenching (NPQ) is a photoprotective mechanism in light-harvesting antennae. NPQ is triggered by chloroplast thylakoid lumen acidification and is accompanied by violaxanthin de-epoxidation to zeaxanthin, which further stimulates NPQ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a337b246ea8c768ea8f73fc7646e630b
https://pubmed.ncbi.nlm.nih.gov/26988983
https://pubmed.ncbi.nlm.nih.gov/26988983
Autor:
Sarah, Hollingshead, Jana, Kopečná, David R, Armstrong, Lenka, Bučinská, Philip J, Jackson, Guangyu E, Chen, Mark J, Dickman, Michael P, Williamson, Roman, Sobotka, C Neil, Hunter
Publikováno v:
Frontiers in Plant Science
In the chlorophyll (Chl) biosynthesis pathway the formation of protochlorophyllide is catalyzed by Mg-protoporphyrin IX methyl ester (MgPME) cyclase. The Ycf54 protein was recently shown to form a complex with another component of the oxidative cycla
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::119fc3e74f46237faedd0925fb2346c4
https://pubmed.ncbi.nlm.nih.gov/27014315
https://pubmed.ncbi.nlm.nih.gov/27014315
Autor:
Amanda A. Brindley, Israel Cabeza de Vaca, Nathan B. P. Adams, Victor Guallar, Jana Kopečná, C. Neil Hunter, Paul A. Davison, Roman Sobotka
Publikováno v:
UPCommons. Portal del coneixement obert de la UPC
Universitat Politècnica de Catalunya (UPC)
Recercat. Dipósit de la Recerca de Catalunya
instname
Universitat Politècnica de Catalunya (UPC)
Recercat. Dipósit de la Recerca de Catalunya
instname
In oxygenic phototrophs, chlorophylls, hemes and bilins are synthesized by a common branched pathway. Given the phototoxic nature of tetrapyrroles, this pathway must be tightly regulated and an important regulatory role is attributed to Mgchelatase e
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0219e0c4b3eabfadd0abd22a3a25fc84
https://hdl.handle.net/2117/84233
https://hdl.handle.net/2117/84233
Publikováno v:
BIO-PROTOCOL. 5
[Abstract] Intermediates of tetrapyrrole biosynthetic pathway are low-abundant compounds, and their quantification is usually difficult, time consuming and requires large amounts of input material. Here, we describe a method allowing fast and accurat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2452fc5991f5c9fa390de943cc74aabf
https://doi.org/10.21769/bioprotoc.1390
https://doi.org/10.21769/bioprotoc.1390