Bifunctional Non-Canonical Amino Acids: Combining Photo-Crosslinking with Click Chemistry

Autor: Jan-Erik Hoffmann

Publikováno v: Biomolecules, Vol 10, Iss 4, p 578 (2020)

Genetic code expansion is a powerful tool for the study of protein interactions, as it allows for the site-specific incorporation of a photoreactive group via non-canonical amino acids. Recently, several groups have published bifunctional amino acids

Externí odkaz: https://doaj.org/article/1b053f72253d4ab891f070f4a682ffec

The Highly Potent AhR Agonist Picoberin Modulates Hh-Dependent Osteoblast Differentiation

Autor: Jana Flegel, Saad Shaaban, Zhi Jun Jia, Britta Schulte, Yilong Lian, Adrian Krzyzanowski, Malte Metz, Tabea Schneidewind, Fabian Wesseler, Anke Flegel, Alisa Reich, Alexandra Brause, Gang Xue, Minghao Zhang, Lara Dötsch, Isabelle D. Stender, Jan-Erik Hoffmann, Rebecca Scheel, Petra Janning, Fraydoon Rastinejad, Dennis Schade, Carsten Strohmann, Andrey P. Antonchick, Sonja Sievers, Pedro Moura-Alves, Slava Ziegler, Herbert Waldmann

Publikováno v: Journal of Medicinal Chemistry. 65:16268-16289

Identification and analysis of small molecule bioactivity in target-agnostic cellular assays and monitoring changes in phenotype followed by identification of the biological target are a powerful approach for the identification of novel bioactive che

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::be09301612a26f71d7b6806277269021
https://doi.org/10.1021/acs.jmedchem.2c00956

Neutralizing antibody responses 300 days after SARS-CoV-2 infection and induction of high antibody titers after vaccination

Autor: Doris Urlaub, Natalie Wolfsdorff, Jan‐Erik Hoffmann, Stefanie Dorok, Markus Hoffmann, Moritz Anft, Naomi Pieris, Patrick Günther, Bernhard Schaaf, Uwe Cassens, Peter Bröde, Maren Claus, Lea K. Picard, Sabine Wingert, Simone Backes, Deniz Durak, Nina Babel, Stefan Pöhlmann, Frank Renken, Stefan Raunser, Carsten Watzl

Publikováno v: European journal of immunology, 52(5):810-815

Neutralizing antibodies against SARS-CoV-2 are important to protect against infection and/or disease. Using an assay to detect antibodies directed against the receptor binding domain (RBD) of SARS-CoV-2 Spike, we identified individuals with SARS-CoV-

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9a09bae60f6c3c05d38b532f2443191c
Wiley Online Library

A Genetically Encoded Diazirine Analogue for RNA–Protein Photo‐crosslinking

Autor: Matthias W. Hentze, Dmytro Dziuba, Carsten Schultz, Jan Erik Hoffmann

Publikováno v: Chembiochem

Ultraviolent crosslinking is a key experimental step in the numerous protocols that have been developed for capturing and dissecting RNA–protein interactions in living cells. UV crosslinking covalently stalls dynamic interactions between RNAs and t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d06fc3f1c99b9e8786a9a6b2d1621af5
https://doi.org/10.1002/cbic.201900559

Death-Associated Protein Kinase Activity Is Regulated by Coupled Calcium/Calmodulin Binding to Two Distinct Sites

Autor: Dmitri I. Svergun, Andrew A. McCarthy, Bertrand Simon, Carsten Schultz, Juha Vahokoski, Hayretin Yumerefendi, Petri Kursula, Haydyn D. T. Mertens, Jan Erik Hoffmann, Matthias Wilmanns, Dana Komadina, Anne-Sophie Huart, Darren J. Hart, Koen Temmerman

Publikováno v: Structure (London, England : 1993)
Structure (London, England : 1993), 2016, 24 (6), pp.851-61. ⟨10.1016/j.str.2016.03.020⟩
Structure(London, England:1993)

Summary The regulation of many protein kinases by binding to calcium/calmodulin connects two principal mechanisms in signaling processes: protein phosphorylation and responses to dose- and time-dependent calcium signals. We used the calcium/calmoduli

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e91a1e3906af28b7a3ccd6ac9c47582