Zobrazeno 1 - 10
of 35
pro vyhledávání: '"Jan Zimak"'
Autor:
Aude I. Segaliny, Jayapriya Jayaraman, Xiaoming Chen, Jonathan Chong, Ryan Luxon, Audrey Fung, Qiwei Fu, Xianzhi Jiang, Rodrigo Rivera, Xiaoya Ma, Ci Ren, Jan Zimak, Per Niklas Hedde, Yonglei Shang, George Wu, Weian Zhao
Publikováno v:
Communications Biology, Vol 6, Iss 1, Pp 1-14 (2023)
A single-cell based bispecific antibody (BsAb) discovery pipeline, based on a microfluidics droplets generation and sorting systems, has the potential to speed up the discovery and development of functional antibodies such as BsAb therapeutics.
Externí odkaz:
https://doaj.org/article/7a14b50910074c84bb0bfc0a503c1bb8
Autor:
Tam Vu, Alexander Vallmitjana, Joshua Gu, Kieu La, Qi Xu, Jesus Flores, Jan Zimak, Jessica Shiu, Linzi Hosohama, Jie Wu, Christopher Douglas, Marian L. Waterman, Anand Ganesan, Per Niklas Hedde, Enrico Gratton, Weian Zhao
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-14 (2022)
Spatial-omics methods with ease-of-use and high multiplexing are in demand. Here the authors report Multi Omic Single-scan Assay with Integrated Combinatorial Analysis (MOSAICA) which uses Spectral and Fluorescence Lifetime Imaging and Microscopy; th
Externí odkaz:
https://doaj.org/article/3a42cb1c065c4f01927be67c3c3c58b9
Autor:
Jan Zimak, Zachary W. Wagoner, Nellie Nelson, Brooke Waechtler, Hana Schlosser, Morgan Kopecky, Jie Wu, Weian Zhao
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
Abstract We report that epigenetic silencing causes the loss of function of multi-transcript unit constructs that are integrated using CRISPR-Cas9. Using a modular two color reporter system flanked by selection markers, we demonstrate that expression
Externí odkaz:
https://doaj.org/article/99a5ea87ae6540f48bce4ae7dceb0557
Autor:
Monika K. Singha, Jan Zimak, Samantha R. Levine, Nan Dai, Chan Hong, Cecily Anaraki, Mrityunjay Gupta, Christopher J. Halbrook, Scott X. Atwood, Robert C. Spitale
Publikováno v:
Biochemistry. 61:2638-2642
Autor:
Edward B. Samson, David S. Tsao, Jan Zimak, R. Tyler McLaughlin, Nicholaus J. Trenton, Emily M. Mace, Jordan S. Orange, Volker Schweikhard, Michael R. Diehl
Publikováno v:
Biology Open, Vol 6, Iss 6, Pp 785-799 (2017)
IQGAP1 is a large, multi-domain scaffold that helps orchestrate cell signaling and cytoskeletal mechanics by controlling interactions among a spectrum of receptors, signaling intermediates, and cytoskeletal proteins. While this coordination is known
Externí odkaz:
https://doaj.org/article/f292250d8fa2446fbe40ac69e66c7c58
Autor:
Ci Ren, Xiaoming Chen, Per Niklas Hedde, Weian Zhao, George Wu, Jan Zimak, Qiwei Fu, Audrey W. Fung, Jonathan Chong, Aude I. Segaliny, Rodrigo Rivera, Yonglei Shang, Jayapriya Jayaraman, Ryan Luxon, Xiaoya Ma, Xianzhi Jiang
Publikováno v:
Communications biology, vol 6, iss 1
Bispecific antibodies (BsAbs) represent an emerging class of immunotherapy but inefficiency in the current BsAb discovery paradigm has limited their broad clinical availability. Here we report a high throughput, agnostic, single-cell-based BsAb funct
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5502b0a111d753b723aa6df06f0c77e5
https://escholarship.org/uc/item/8fs1g0vp
https://escholarship.org/uc/item/8fs1g0vp
Publikováno v:
Chembiochem : a European journal of chemical biology, vol 23, iss 19
Chembiochem
Chembiochem
Herein we present the exploration of the utility of DNA demethylase enzymes for targeted protein degradation. Novel benzylguanine substrates are characterized for their ability to control protein degradation in cells. Our data demonstrate the utility
Autor:
Enrico Gratton, Christopher Ryan Douglas, Jie Wu, Per Niklas Hedde, Joshua Gu, Qi Xu, Marian L. Waterman, Anand N. Ganesan, Linzi Hosohama, Jessica Shiu, Tam Vu, Jesus Flores, Alexander Vallmitjana, Jan Zimak, Kieu Thai La, Weian Zhao
Multiplexed mRNA profiling in the spatial context provides important new information enabling basic research and clinical applications. Unfortunately, most existing spatial transcriptomics methods are limited due to either low multiplexing or assay c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::674be7b492ab05099490d4f7c0636bba
https://doi.org/10.1101/2021.06.22.449468
https://doi.org/10.1101/2021.06.22.449468
Autor:
Morgan Kopecky, Brooke Waechtler, Hana Schlosser, Jan Zimak, Jie Wu, Zachary W. Wagoner, Nellie Nelson, Weian Zhao
Publikováno v:
Scientific reports, vol 11, iss 1
Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
Scientific Reports
Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
Scientific Reports
We report that epigenetic silencing causes the loss of function of multi-transcript unit constructs that are integrated using CRISPR-Cas9. Using a modular two color reporter system flanked by selection markers, we demonstrate that expression heteroge
Publikováno v:
ISBI
Bacteria entering the bloodstream causes bloodstream infection (BSI). Without proper treatment, BSI can lead to sepsis which is a life-threatening condition. Detection of bacteria in blood at the early stages of BSI can effectively prevent the develo