Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Jan Walther Perthold"'
Autor:
Gordana Wozniak-Knopp, Gerhard Stadlmayr, Jan Walther Perthold, Katharina Stadlbauer, Mathias Gotsmy, Stefan Becker, Florian Rüker
Publikováno v:
PLoS ONE, Vol 13, Iss 4, p e0195442 (2018)
We have designed a complete antibody-like construct where the CH1 and Cκ domains are exchanged for a pair of the CH3 domains and efficient pairing of the heavy and light variable domain is achieved using "Knobs-into-Holes" strategy. This construct,
Externí odkaz:
https://doaj.org/article/6fd29778184a4a0396484ba2c6136214
Publikováno v:
Journal of Chemical Theory and Computation
Currently, two different methods dominate the field of biomolecular free-energy calculations for the prediction of binding affinities. Pathway methods are frequently used for large ligands that bind on the surface of a host, such as protein–protein
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee8aa009b0c377016af3f3ddf44662d0
https://doi.org/10.1021/acs.jctc.0c00719
https://doi.org/10.1021/acs.jctc.0c00719
Publikováno v:
Journal of Chemical Information and Modeling
Free-energy perturbation (FEP) methods are commonly used in drug design to calculate relative binding free energies of different ligands to a common host protein. Alchemical ligand transformations are usually performed in multiple steps which need to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e44b1335bd921a3f601e124838b5bef
https://doi.org/10.1021/acs.jcim.0c00456
https://doi.org/10.1021/acs.jcim.0c00456
Publikováno v:
Journal of Chemical Information and Modeling. 59:5074-5085
Protein-protein docking algorithms promise a potential relief for the mismatch between the number of experimentally determined complex structures and the number of relevant protein interactions in an organism. To distinguish correctly from wrongly ge
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::06712f7240e6e1861135e8187726071e
https://doi.org/10.1021/acs.jcim.9b00687
https://doi.org/10.1021/acs.jcim.9b00687
Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptor
Autor:
Janine Niederhoefer, Elisabeth Laurent, Daniel Maresch, Ali Mirazimi, Esther Foederl-Hoebenreich, Tuemay Capraz, Kurt Zatloukal, Clemens Gruenwald-Gruber, Gerald Wirnsberger, Josef M. Penninger, Chris Oostenbrink, Vanessa Monteil, Johannes Stadlmann, Jan Walther Perthold, Nikolaus F. Kienzl, Lukas Mach
Infection and viral entry of SARS-CoV-2 crucially depends on the binding of its Spike protein to angiotensin converting enzyme 2 (ACE2) presented on host cells. Glycosylation of both proteins is critical for this interaction. Recombinant soluble huma
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::aba90ad8e8d20ac5f2ad745813462185
https://doi.org/10.1101/2021.08.31.458325
https://doi.org/10.1101/2021.08.31.458325
Autor:
Felicitas Oberndorfer, Nuria Montserrat, Ali Mirazimi, Sylvia Knapp, Lisabeth Pimenov, Anna Ohradanova-Repic, Karin Lakovits, Shane J. F. Cronin, Riem Gawish, Gerald Wirnsberger, Hannes Stockinger, Josef M. Penninger, Anastasiya Hladik, Tuemay Capraz, Louis Boon, Chris Oostenbrink, Andreas Bergthaler, Jan Walther Perthold, Astrid Hagelkruys, Philipp Starkl, Lukas Endler, Benedikt Agerer
SummaryDespite tremendous progress in the understanding of COVID-19, mechanistic insight into immunological, disease-driving factors remains limited. We generated maVie16, a mouse-adapted SARS-CoV-2, by serial passaging of a human isolate. In silico
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::03e29a07eb0be9795d5eaa946ebe9a91
https://doi.org/10.1101/2021.08.09.455606
https://doi.org/10.1101/2021.08.09.455606
Publikováno v:
The journal of physical chemistry. B. 122(19)
Enveloping distribution sampling (EDS) is an efficient approach to calculate multiple free-energy differences from a single molecular dynamics (MD) simulation. However, the construction of an appropriate reference-state Hamiltonian that samples all s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0defe5fe9c796e749f296dbd03a2c958
https://pubmed.ncbi.nlm.nih.gov/29669415
https://pubmed.ncbi.nlm.nih.gov/29669415
Autor:
Florian Rüker, Mathias Gotsmy, Gordana Wozniak-Knopp, Jan Walther Perthold, Katharina Stadlbauer, Gerhard Stadlmayr, Stefan Becker
Publikováno v:
PLoS ONE, Vol 13, Iss 4, p e0195442 (2018)
PLoS ONE
PLoS ONE
We have designed a complete antibody-like construct where the CH1 and Cκ domains are exchanged for a pair of the CH3 domains and efficient pairing of the heavy and light variable domain is achieved using “Knobs-into-Holes” strategy. This constru
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22383a93e7f7750ef664be4c85b10db2
http://europepmc.org/articles/PMC5891013?pdf=render
http://europepmc.org/articles/PMC5891013?pdf=render
Autor:
Maximilian Woisetschläger, Jan Walther Perthold, Haijun Sun, Florian Rüker, Katharina Stadlbauer, Gerhard Stadlmayr, Gordana Wozniak-Knopp
Publikováno v:
Protein engineering, designselection : PEDS. 30(9)
Fc fragment with antigen-binding (Fcab) is a novel construct which can be selected to recognize specifically a wide variety of target proteins. We describe the selection and affinity maturation of Fcab clones targeting VEGF, an important pro-angiogen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::86b81ce74437def8d80695a2b75e55a1
https://pubmed.ncbi.nlm.nih.gov/28981753
https://pubmed.ncbi.nlm.nih.gov/28981753
Autor:
Florian Rueker, Jan Walther Perthold, Katharina Stadlbauer, Gerhard Stadlmayr, Gordana Wozniak Knopp
Publikováno v:
New Biotechnology. 33:S47
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6111d7bad4412cd131e85b8e7173b136
https://doi.org/10.1016/j.nbt.2016.06.887
https://doi.org/10.1016/j.nbt.2016.06.887