Hypoxic 3D in vitro culture models reveal distinct resistance processes to TKIs in renal cancer cells

Autor: Zofia F. Bielecka, Agata Malinowska, Klaudia K. Brodaczewska, Aleksandra Klemba, Claudine Kieda, Paweł Krasowski, Elżbieta Grzesiuk, Jan Piwowarski, Anna M. Czarnecka, Cezary Szczylik

Publikováno v: Cell & Bioscience, Vol 7, Iss 1, Pp 1-18 (2017)

Abstract Background The aim of this study is to determine the effect of hypoxia on axitinib and sorafenib-treated renal cell carcinoma (RCC) cells. Hypoxia is a crucial factor influencing transcription process via protein modulation, which was shown

Externí odkaz: https://doaj.org/article/2594bf1a9948458d94bdc77edbcb02ab

Novel AlkB dioxygenases--alternative models for in silico and in vivo studies.

Autor: Damian Mielecki, Dorota Ł Zugaj, Anna Muszewska, Jan Piwowarski, Aleksandra Chojnacka, Marcin Mielecki, Jadwiga Nieminuszczy, Marcin Grynberg, Elżbieta Grzesiuk

Publikováno v: PLoS ONE, Vol 7, Iss 1, p e30588 (2012)

BackgroundALKBH proteins, the homologs of Escherichia coli AlkB dioxygenase, constitute a direct, single-protein repair system, protecting cellular DNA and RNA against the cytotoxic and mutagenic activity of alkylating agents, chemicals significantly

Externí odkaz: https://doaj.org/article/e753042fc77542ea8e75a36a18874a4c

Replacing centrifugation with mixing in urine analysis enriches protein pool in the urine samples

Autor: Jan Piwowarski, Michał Marcinkowski, Tomaš Pilžys, Damian Garbicz, Elżbieta Grzesiuk, Wojciech Lisik, Radosław Cylke, Maciej Kosieradzki, Iga Wiśniowski, Małgorzata Grzesiuk

Publikováno v: Analytical biochemistry. 628

Urine is the basic diagnostic material, easy to collect, not requiring invasive approach. During standard procedure the urine samples are centrifuged and the supernatant analysed physically, biochemically, and microscopically. The centrifugation step

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1a369af277a65e95129fa1ca41bb7639
https://pubmed.ncbi.nlm.nih.gov/34111418

Calmodulin as Ca2+-Dependent Interactor of FTO Dioxygenase

Autor: Jarosław Poznański, Damian Garbicz, Elżbieta Grzesiuk, Karolina Ferenc, Oleksandr Skorobogatov, Jan Piwowarski, Maria Winiewska-Szajewska, Michał Marcinkowski, Kaja Przygońska, Tomaš Pilžys

Publikováno v: International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 22, Iss 10869, p 10869 (2021)
Volume 22
Issue 19

FTO is an N6-methyladenosine demethylase removing methyl groups from nucleic acids. Several studies indicate the creation of FTO complexes with other proteins. Here, we looked for regulatory proteins recognizing parts of the FTO dioxygenase region. I

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::698b08e5b38eb5ad29937d0bc3d84aa5
https://doi.org/10.3390/ijms221910869

ALKBH overexpression in head and neck cancer: potential target for novel anticancer therapy

Autor: Tomaš Pilžys, Ewa Migacz, Arne Klungland, Jarosław Poznański, Jan Piwowarski, Małgorzata Dylewska, Damian Garbicz, Karolina Ferenc, Wojciech Kukwa, Andrzej Kukwa, Damian Mielecki, Elżbieta Grzesiuk, Michał Marcinkowski, Dominika Wolosz, Adam Mieczkowski

Publikováno v: Scientific Reports, Vol 9, Iss 1, Pp 1-16 (2019)
Scientific Reports

The nine identified human homologues of E. coli AlkB 2-oxoglutarate (2OG) and Fe(II)-dependent dioxygenase, ALKBH1-8 and FTO, display different substrate specificities and diverse biological functions. Here we discovered the combined overexpression o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b379f8b2e81fd503e181756d50a790e8
https://doi.org/10.1038/s41598-019-49550-x