A novel thermostable prokaryotic fucoidan active sulfatase PsFucS1 with an unusual quaternary hexameric structure

Autor: Maria Dalgaard Mikkelsen, Hang Thi Thuy Cao, Thomas Roret, Nanna Rhein-Knudsen, Jesper Holck, Van Thi Thanh Tran, Thuan Thi Nguyen, Vy Ha Nguyen Tran, Mateusz Jakub Lezyk, Jan Muschiol, Thinh Duc Pham, Mirjam Czjzek, Anne S. Meyer

Publikováno v: Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)

Abstract Fucoidans are sulfated, fucose-rich marine polysaccharides primarily found in cell walls of brown seaweeds (macroalgae). Fucoidans are known to possess beneficial bioactivities depending on their structure and sulfation degree. Here, we repo

Externí odkaz: https://doaj.org/article/cc38177e7dd745e28e3da788539a2bda

Improvement of the Transglycosylation Efficiency of a Lacto-N-Biosidase from Bifidobacterium bifidum by Protein Engineering

Autor: Marlene Vuillemin, Jesper Holck, Martin Matwiejuk, Eduardo S. Moreno Prieto, Jan Muschiol, Dora Molnar-Gabor, Anne S. Meyer, Birgitte Zeuner

Publikováno v: Applied Sciences, Vol 11, Iss 23, p 11493 (2021)

The lacto-N-biosidase LnbB from Bifidobacterium bifidum JCM 1254 was engineered to improve its negligible transglycosylation efficiency with the purpose of enzymatically synthesizing lacto-N-tetraose (LNT; Gal-β1,3-GlcNAc-β1,3-Gal-β1,4-Glc) in one

Externí odkaz: https://doaj.org/article/7942c6cd56aa423dbdc1d68c9884bde3

Synthesis of Human Milk Oligosaccharides: Protein Engineering Strategies for Improved Enzymatic Transglycosylation

Autor: Birgitte Zeuner, David Teze, Jan Muschiol, Anne S. Meyer

Publikováno v: Molecules, Vol 24, Iss 11, p 2033 (2019)

Human milk oligosaccharides (HMOs) signify a unique group of oligosaccharides in breast milk, which is of major importance for infant health and development. The functional benefits of HMOs create an enormous impetus for biosynthetic production of HM

Externí odkaz: https://doaj.org/article/70aa65b73984490db2ba9869c6555e54

A novel thermostable prokaryotic fucoidan active sulfatase PsFucS1 with an unusual quaternary hexameric structure

Autor: Mirjam Czjzek, Anne S. Meyer, Van Thi Thanh Tran, Thuan Thi Nguyen, Thomas Roret, Nanna Rhein-Knudsen, Vy Ha Nguyen Tran, Jesper Holck, Thinh Duc Pham, Jan Muschiol, Hang Thi Thuy Cao, Maria Dalgaard Mikkelsen, Mateusz Jakub Lezyk

Publikováno v: Scientific Reports
Scientific Reports, Nature Publishing Group, 2021, 11 (1), ⟨10.1038/s41598-021-98588-3⟩
Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
Mikkelsen, M D, Cao, H T T, Roret, T, Rhein-Knudsen, N, Holck, J, Tran, V T T, Nguyen, T T, Tran, V H N, Lezyk, M J, Muschiol, J, Pham, T D, Czjzek, M & Meyer, A S 2021, ' A novel thermostable prokaryotic fucoidan active sulfatase PsFucS1 with an unusual quaternary hexameric structure ', Scientific Reports, vol. 11, 19523 . https://doi.org/10.1038/s41598-021-98588-3

Fucoidans are sulfated, fucose-rich marine polysaccharides primarily found in cell walls of brown seaweeds (macroalgae). Fucoidans are known to possess beneficial bioactivities depending on their structure and sulfation degree. Here, we report the fi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::62dc11e1d81c498f2731c0d306def462
https://doi.org/10.1038/s41598-021-98588-3

Improved Transglycosylation by a Xyloglucan-Active α-l-Fucosidase from Fusarium graminearum

Autor: Jan Muschiol, Birgitte Zeuner, Anne S. Meyer, Jesper Holck, Marlene Vuillemin

Publikováno v: Journal of Fungi, Vol 6, Iss 295, p 295 (2020)
Journal of Fungi
Volume 6
Issue 4
Zeuner, B, Vuillemin, M, Holck, J, Muschiol, J & Meyer, A S 2020, ' Improved Transglycosylation by a Xyloglucan-Active α-l-Fucosidase from Fusarium graminearum ', Journal of Fungi, vol. 6, no. 4, 295 . https://doi.org/10.3390/jof6040295

Fusarium graminearum produces an &alpha
l-fucosidase, FgFCO1, which so far appears to be the only known fungal GH29 &alpha
l-fucosidase that catalyzes the release of fucose from fucosylated xyloglucan. In our quest to synthesize bioactive g

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d0f97f87f8567eb95de2985b5f743ee1
https://www.mdpi.com/2309-608X/6/4/295