Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Jan Manchak"'
Publikováno v:
Journal of Bacteriology. 192:1730-1734
F plasmid TraF and TraH are required for F pilus assembly and F plasmid transfer. Using flotation sucrose density gradients, we found that TraF and TraH (as well as TraU and TraW) localized to the outer membrane in the presence of the complete F tran
Publikováno v:
Molecular Microbiology. 70:89-99
F plasmid-mediated bacterial conjugation requires interactions between a relaxosome component, TraM, and the coupling protein TraD, a hexameric ring ATPase that forms the cytoplasmic face of the conjugative pore. Here we present the crystal structure
Publikováno v:
Microbiology. 153:442-451
The mating pair stabilization (Mps) protein of the F plasmid, TraG, is unique to F-like type IV secretion systems. TraG is a polytopic inner-membrane protein with a large C-terminal periplasmic domain that is required for piliation and Mps, whereas t
Autor:
J. N. Mark Glover, Paul G. Scott, Jan Manchak, Jun Lu, Laura S. Frost, Joyce J. W. Wong, Ross A. Edwards
Publikováno v:
The EMBO Journal. 25:2930-2939
TraM is essential for F plasmid-mediated bacterial conjugation, where it binds to the plasmid DNA near the origin of transfer, and recognizes a component of the transmembrane DNA transfer complex, TraD. Here we report the 1.40 Å crystal structure o
Publikováno v:
ResearcherID
F and R100-1 are closely related, derepressed, conjugative plasmids from the IncFI and IncFII incompatibility groups, respectively. Heteroduplex mapping and genetic analyses have revealed that the transfer regions are extremely similar between the tw
Autor:
Seema Endley, Pushpa Kathir, Kesmanee Maneewannakul, Jan Manchak, Deanna Moore, Laura S. Frost, Karin Ippen-Ihler
Publikováno v:
Molecular Microbiology. 22:197-205
A derivative of the F plasmid, pOX38-tra715, expresses the entire F tra operon from a foreign promoter (PT7) derived from phage T7. A series of plasmids related to pOX38-tra715 were constructed which carry either deletion mutations or point mutations
Publikováno v:
Molecular microbiology. 70(1)
F plasmid-mediated bacterial conjugation requires interactions between a relaxosome component, TraM, and the coupling protein TraD, a hexameric ring ATPase that forms the cytoplasmic face of the conjugative pore. Here we present the crystal structure
Publikováno v:
The FASEB Journal. 20
Autor:
Jan Manchak, Koto Hayakawa, Trevor C. Elton, Samantha J. Holland, Bart Hazes, Laura S. Frost, Gerald F. Audette
Publikováno v:
Acta crystallographica. Section D, Biological crystallography. 60(Pt 11)
TraF, a component of the Escherichia coli type IV secretory system, has been crystallized and preliminary X-ray diffraction data have been collected. TraF is a 26 kDa protein encoded by the E. coli F plasmid and is required for conjugative plasmid tr
Autor:
Jun Lu, Laura S. Frost, Colin Davidson, Ronald A. Skurray, William A. Klimke, Jan Manchak, Neville Firth
Publikováno v:
Plasmid. 48(1)
pED208 is a transfer-derepressed mutant of the IncFV plasmid, F 0 lac , which has an IS 2 element inserted in its traY gene, resulting in constitutive overexpression of its transfer ( tra ) region. The pED208 transfer region, which encodes proteins r