Zobrazeno 1 - 10
of 19
pro vyhledávání: '"Jan Kostal"'
Publikováno v:
Molecular and Biochemical Parasitology. 158:163-175
Leishmania amazonensis was found to release nucleoside diphosphate kinase (NdK)-a stable enzyme capable of decreasing extracellular ATP. The release of this enzyme from Leishmania results in its progressive accumulation extracellularly as they replic
Publikováno v:
Nature Protocols. 2:1263-1268
This protocol presents a new method to purify plasmid DNA using temperature-triggered precipitation. The principle is based on the specific DNA-binding affinity of a bacterial metalloregulatory (MerR) protein to its cognate DNA sequence and the tempe
Autor:
Wilfred Chen, U Loi Lao, Alin Chen, Ashok Mulchandani, Mark R. Matsumoto, Giridhar Prabhukumar, Jan Kostal
Publikováno v:
Journal of Nanoparticle Research. 7:517-523
Nanoscale materials have been gaining increasing interest in the area of environmental remediation because of their unique physical, chemical and biological properties. One emerging area of research has been the development of novel materials with in
Publikováno v:
Applied and Environmental Microbiology. 70:4582-4587
The metalloregulatory protein ArsR, which offers high affinity and selectivity toward arsenite, was overexpressed in Escherichia coli in an attempt to increase the bioaccumulation of arsenic. Overproduction of ArsR resulted in elevated levels of arse
Publikováno v:
Biotechnology and Bioengineering. 85:293-297
This report describes a new plasmid DNA purification method, which takes advantage of the DNA-binding affinity and specificity of the bacterial metalloregulatory protein MerR, and of the temperature responsiveness of elastin-like proteins (ELPs). Upo
Publikováno v:
Applied and Environmental Microbiology. 69:3176-3180
The metalloregulatory protein MerR, which exhibits high affinity and selectivity toward mercury, was exploited for the construction of microbial biosorbents specific for mercury removal. Whole-cell sorbents were constructed with MerR genetically engi
Publikováno v:
International Biodeterioration & Biodegradation. 45:27-33
The capacity of a range of aliphatic alkanes (C 6 –C 16 ), intermediates of n -decane oxidation and sodium dodecyl sulphate (SDS) to induce decane-mineralization activity in the cells of Pseudomonas C12B was compared with that for n -decane. The co
Autor:
Dani L. McBeth, Miloslav Suchanek, Hana Klierova, Jan Kostal, Katerina Demnerova, Blanka Kralova
Publikováno v:
International Biodeterioration & Biodegradation. 42:221-228
Pseudomonas C12B is able to degrade alkyl sulfates, alkylbenzene sulfonates, and linear alkanes and alkenes. Mitomycin C curing experiments and conjugation experiments demonstrated that the ability to utilize n -alkanes (C 9 –C 12 ) and n -alkenes
Publikováno v:
Pure and Applied Chemistry. 69:2357-2370
Mixtures of indigenous soil bacteria were applied to remediate local ground waters and soil polluted with petroleum derived substances. Implementation of three months remediation protocols resulted in a decline of the amount of petroleum derived cont
Publikováno v:
Biotechnology Letters. 17:765-770
Pseudomonas C12B (NCIMB 11753) is able to utilize a broad range of alkyl sulfates. The growth on n-alkanes of different chain lenght (C6–C16) was tested. Pseudomonas C12B assimilated hydrocarbons from C9–C16. Growth rate on n-decane (1%) that was