Zobrazeno 1 - 10
of 21
pro vyhledávání: '"Jan Huebinger"'
Comparison of Different Materials for Self-Pressurized Vitrification of Feline Oocytes—First Results
Publikováno v:
Animals, Vol 11, Iss 5, p 1314 (2021)
Cryobanking is a crucial part on species conservation. Nowadays, there is no suitable protocol for vitrification of feline oocytes. Self-pressurized rapid freezing of different cell types proved to mimic the advantages of high pressure freezing. As t
Externí odkaz:
https://doaj.org/article/77f8bd83dde644b8ae7da6793fbd9a58
Autor:
Jan Huebinger
Publikováno v:
PLoS ONE, Vol 13, Iss 10, p e0205520 (2018)
Rapid cooling and re-warming has been shown promising to cryopreserve living cells, which cannot be preserved by conventional slow freezing methods. However, success is limited by the cytotoxicity of highly concentrated cryoprotective agents. Recent
Externí odkaz:
https://doaj.org/article/e094c52157d8454ca40db2532d2b8cb2
Publikováno v:
Bio-Protocol, Vol 7, Iss 8 (2017)
Fluorescence live-cell imaging by single molecule localization microscopy (SMLM) or fluorescence lifetime imaging microscopy (FLIM) in principle allows for the spatio-temporal observation of molecular patterns in individual, living cells. However, th
Externí odkaz:
https://doaj.org/article/2127c0012e774b99abac4744d4140992
Publikováno v:
PLoS ONE, Vol 11, Iss 10, p e0164270 (2016)
Rapid cooling of aqueous solutions is a useful approach for two important biological applications: (I) cryopreservation of cells and tissues for long-term storage, and (II) cryofixation for ultrastructural investigations by electron and cryo-electron
Externí odkaz:
https://doaj.org/article/5354385007e54392a061fa2075a8028b
Autor:
Maitreyi S Joshi, Angel Stanoev, Jan Huebinger, Birga Soetje, Veronika Zorina, Lisaweta Roßmannek, Kirsten Michel, Sven AH Müller, Philippe IH Bastiaens
Publikováno v:
The EMBO Journal. 42
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::809fdfabdc101dadead67a0bd5f706fd
https://doi.org/10.15252/embj.2022111806
https://doi.org/10.15252/embj.2022111806
Autor:
Maitreyi S. Joshi, Angel Stanoev, Birga Soetje, Jan Huebinger, Veronika Zorina, Lisaweta Roßmannek, Kirsten Michel, Philippe I. H. Bastiaens
Spatially-organized interaction dynamics between proto-oncogenic epidermal growth factor receptor (EGFR) and protein tyrosine phosphatases (PTPs) determine EGFR’s phosphorylation response to growth factors and thereby cellular behavior within devel
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::11db73736d5dd53c38039d68f69a8263
https://doi.org/10.1101/2022.06.01.494340
https://doi.org/10.1101/2022.06.01.494340
Autor:
Jan Huebinger, Hernan Grecco, Martín E. Masip, Jens Christmann, Günter R. Fuhr, Philippe I. H. Bastiaens
Publikováno v:
Science Advances
Description
Fluorescence imaging under cryo-arrest allows resolution of dynamic signaling system features that are otherwise obscured.
Imaging molecular patterns in cells by fluorescence micro- or nanoscopy has the potential to relate colle
Fluorescence imaging under cryo-arrest allows resolution of dynamic signaling system features that are otherwise obscured.
Imaging molecular patterns in cells by fluorescence micro- or nanoscopy has the potential to relate colle
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1cffdb12e7f2fe32a802190acc0ab852
https://pubmed.ncbi.nlm.nih.gov/34890224
https://pubmed.ncbi.nlm.nih.gov/34890224
Publikováno v:
Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
Scientific Reports
Scientific Reports
To understand cellular functionalities, it is essential to unravel spatio-temporal patterns of molecular distributions and interactions within living cells. The technological progress in fluorescence microscopy now allows in principle to measure thes
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::61b9038f4c2a362000c71ddd6387ed9f
http://link.springer.com/article/10.1038/s41598-018-36112-w
http://link.springer.com/article/10.1038/s41598-018-36112-w
Publikováno v:
Physical review letters. 125(6)
Glycerol pressurized to 2 kbar and hyperquenched from the bulk liquid at rates of about $\ensuremath{-}10\text{ }000\text{ }\text{ }\mathrm{K}/\mathrm{s}$, has been frozen to an extreme out-of-equilibrium state. As compared to conventionally cooled m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc5d3e0e6c7fb05bd5a470cd522c0a3a
https://pubmed.ncbi.nlm.nih.gov/32845696
https://pubmed.ncbi.nlm.nih.gov/32845696
Autor:
Ola Sabet, Jan Huebinger, Antonios D. Konitsiotis, Philippe I. H. Bastiaens, Günther R Fuhr, Jens Christmann, Frank Wehner, Martin E. Masip
Publikováno v:
Nature Methods. 13:665-672
The dynamics of molecules in living cells hampers precise imaging of molecular patterns by functional and super-resolution microscopy. We developed a method that circumvents lethal chemical fixation and allows on-stage cryo-arrest for consecutive ima
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d418d7643609f7b9458768f3bdc0519
https://doi.org/10.1038/nmeth.3921
https://doi.org/10.1038/nmeth.3921