Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Jan Havliš"'
Publikováno v:
Frontiers in Plant Science, Vol 12 (2021)
Quantitative protein extraction from biological samples, as well as contaminants removal before LC-MS/MS, is fundamental for the successful bottom-up proteomic analysis. Four sample preparation methods, including the filter-aided sample preparation (
Externí odkaz:
https://doaj.org/article/93236b1d89bb486a9aeb9fd4ef89f52f
Publikováno v:
Journal of Chromatography A. 1469:88-95
We studied sequence-dependent retention properties of synthetic 5′-terminal phosphate absent trinucleotides containing adenine, guanine and thymine through reversed-phase liquid chromatography (RPLC) and QSRR modelling. We investigated the influenc
Publikováno v:
European Journal of Mass Spectrometry. 20:255-260
Incorporation of an 18O atom into a peptide C-terminus by proteolytic cleavage in the presence of H218O is one of the most effective ways of enhancing tandem mass spectrometry (MS/MS)-based de novo sequencing. Incorporation is usually accomplished by
Publikováno v:
Journal of Separation Science. 35:3227-3234
Retention characteristics of selected synthetic 5'-terminal phosphate absent penta-nucleotides containing adenine, guanine, and thymine were studied in relation to their sequence by hydrophilic interaction chromatography and ion-interaction reversed-
Publikováno v:
Analytical Biochemistry. 376:94-102
Trypsin (EC 3.4.21.4) is the protease of choice for proteome analysis using mass spectrometry of peptides in sample digests. In this work, trypsin from Streptomyces griseus (SGT) was purified to homogeneity from pronase. The enzyme was evaluated in i
Publikováno v:
Nature Protocols. 1:2856-2860
In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 10-year-old recipe by Shevchenko et al. has been optimized to increase the speed and sensitivity of analysis. The protocol
Thermostable trypsin conjugates for high-throughput proteomics: synthesis and performance evaluation
Autor:
Henrik Thomas, Tat'ána Štosová, Jan Havliš, Marek Šebela, Natalie Wielsch, Andrej Shevchenko, Zbyněk Zdráhal
Publikováno v:
PROTEOMICS. 6:2959-2963
Conjugating bovine trypsin with oligosaccharides maltotriose, raffinose and stachyose increased its thermostability and suppressed autolysis, without affecting its cleavage specificity. These conjugates accelerated the digestion of protein substrates
Autor:
Jan Havliš, Martin Trbušek
Publikováno v:
Journal of Chromatography B. 781:373-392
The extent of the DNA methylation of genomic DNA as well as the methylation pattern of many gene-regulatory areas are important aspects with regard to the state of genetic information, especially their expression. There is growing evidence that aberr
Publikováno v:
Natural Products Analysis: Instrumentation, Methods, and Applications
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e30f41af14ceee6f54aa1f0c56e5164d
https://doi.org/10.1002/9781118876015.ch9
https://doi.org/10.1002/9781118876015.ch9
Autor:
Eberhard Krause, Anne-Lore Schlaitz, Yvonne Gloor, Michael Edmund Beck, Christiane Walch-Solimena, Lars Demmel, Jan Havliš, Peggy P. Hsu, Christian Klose, Andrej Shevchenko, Yannis Kalaidzidis
Publikováno v:
Molecular biology of the cell, 19(3): 1046-1061
The yeast phosphatidylinositol 4-kinase Pik1p is essential for proliferation, and it controls Golgi homeostasis and transport of newly synthesized proteins from this compartment. At the Golgi, phosphatidylinositol 4-phosphate recruits multiple cytoso