Detection of SARS-CoV-2 in exhaled breath from non-hospitalized COVID-19-infected individuals

Autor: Cæcilie Leding, Julia Skov, Katrine Uhrbrand, Jan Gorm Lisby, Katrine Pedersbæk Hansen, Thomas Benfield, Louise Katrine Duncan

Publikováno v: Scientific Reports, Vol 12, Iss 1, Pp 1-8 (2022)

Abstract The diagnosis of COVID-19 is based on detection of SARS-CoV-2 in oro-/nasopharyngel swabs, but due to discomfort and minor risk during the swab procedure, detection of SARS-CoV-2 has been investigated in other biological matrixes. In this pr

Externí odkaz: https://doaj.org/article/7af2f52ffe4c469ea5b7d30d906dd133

A non-enzymatic, isothermal strand displacement and amplification assay for rapid detection of SARS-CoV-2 RNA

Autor: Mohsen Mohammadniaei, Ming Zhang, Jon Ashley, Ulf Bech Christensen, Lennart Jan Friis-Hansen, Rasmus Gregersen, Jan Gorm Lisby, Thomas Lars Benfield, Finn Erland Nielsen, Jens Henning Rasmussen, Ellen Bøtker Pedersen, Anne Christine Rye Olinger, Lærke Tørring Kolding, Maryam Naseri, Tao Zheng, Wentao Wang, Jan Gorodkin, Yi Sun

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)

The reliance on enzymes in SARS-CoV-2 RNA detection imposes limits on transport and storage conditions. Here the authors use non-enzymatic isothermal amplification to detect RNA with no need for reverse transcription.

Externí odkaz: https://doaj.org/article/bd06ee023a57436bb5b1ecd0d8bd16db

Multicenter evaluation of the QIAstat Respiratory Panel-A new rapid highly multiplexed PCR based assay for diagnosis of acute respiratory tract infections.

Autor: Marijo Parčina, Uffe Vest Schneider, Benoit Visseaux, Robert Jozić, Irene Hannet, Jan Gorm Lisby

Publikováno v: PLoS ONE, Vol 15, Iss 3, p e0230183 (2020)

Acute respiratory tract infections (ARTI), including the common cold, pharyngitis, sinusitis, otitis media, bronchiolitis and pneumonia are the most common diagnoses among patients seeking medical care in western countries, and account for most antib

Externí odkaz: https://doaj.org/article/50e4db0579a84335ae666a065a81c8cc

Emergence of circulating influenza A H3N2 viruses with genetic drift in the matrix gene: be alert of false‐negative test results

Autor: Rikke Lind Jørgensen, Christian Johann Lerche, Martin Schou Pedersen, Nikolai Soren Kirkby, Amanda Bolt Botnen, Ramona Trebbien, Stephen Nilsson‐Møller, Mette Pinholt, Alex Christian Yde Nielsen, Henrik Westh, Jan Gorm Lisby, Uffe Vest Schneider

Publikováno v: Jørgensen, R L, Lerche, C J, Pedersen, M S, Kirkby, N S, Botnen, A B, Trebbien, R, Nilsson-Møller, S, Pinholt, M, Nielsen, A C Y, Westh, H, Lisby, J G & Schneider, U V 2022, ' Emergence of circulating influenza A H3N2 viruses with genetic drift in the matrix gene : be alert of false-negative test results ', APMIS, vol. 130, no. 10, pp. 612-617 . https://doi.org/10.1111/apm.13262

In March 2022, we observed samples with a negative fluorescent signal (60.5%, n = 43) for the influenza A matrix gene and a stronger positive signal for subtype A(H3N2). Forty-three samples were positive in InfA (H3N2) (mean Cq 30.9, range 23.9–35.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18a8dc45047af4ce3e5d876045222b1c
https://doi.org/10.1111/apm.13262

A nationwide analytical and clinical evaluation of 44 rapid antigen tests for SARS-CoV-2 compared to RT-qPCR

Autor: Uffe Vest Schneider, Maria Wendelboe Forsberg, Thomas Daell Leineweber, Christel Barker Jensen, Khaled Ghathian, Charlotte Nielsen Agergaard, Kasper Kjersgaard Mortensen, Arieh Cohen, Charlotte Sværke Jørgensen, Helene Larsen, Matilde Bøgelund Hansen, Ulla Saleme, Anders Koch, Nikolai Søren Kirkby, Thomas Kallemose, Marie Louise Schaadt, Frederikke Holm Jensen, Rikke Lind Jørgensen, Chih Man German Ma, Nina Steenhard, Jenny Dahl Knudsen, Jan Gorm Lisby, John Eugenio Coia, Svend Ellermann-Eriksen, Lennart Friis-Hansen, David Fuglsang-Damgaard, Jens Otto Jarløv, Ulrich Stab Jensen, Sanne Jespersen, Ea Sofie Marmolin, Lene Nielsen, Xiaohui Chen Nielsen, Anel Ramic, Lars Jørgen Østergaard

Publikováno v: Schneider, U V, Forsberg, M W, Leineweber, T D, Jensen, C B, Ghathian, K, Agergaard, C N, Mortensen, K K, Cohen, A, Jørgensen, C S, Larsen, H, Hansen, M B, Saleme, U, Koch, A, Kirkby, N S, Kallemose, T, Schaadt, M L, Jensen, F H, Jørgensen, R L, Ma, C M G, Steenhard, N, Knudsen, J D, Lisby, J G & The National Danish RAT testing group 2022, ' A nationwide analytical and clinical evaluation of 44 rapid antigen tests for SARS-CoV-2 compared to RT-qPCR ', Journal of Clinical Virology, vol. 153, 105214 . https://doi.org/10.1016/j.jcv.2022.105214

Background: The SARS-CoV-2 pandemic has resulted in massive testing by Rapid Antigen Tests (RAT) without solid independent data regarding clinical performance being available. Thus, decision on purchase of a specific RAT may rely on manufacturer-prov

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f900301b14d8c752200d7af70ada6e7a
https://orbit.dtu.dk/en/publications/5f3e21b9-96a6-49d5-bf06-30d798d264a0