A high-throughput screen for tuberculosis progression.

Autor: Ralph Carvalho, Jan de Sonneville, Oliver W Stockhammer, Nigel D L Savage, Wouter J Veneman, Tom H M Ottenhoff, Ron P Dirks, Annemarie H Meijer, Herman P Spaink

Publikováno v: PLoS ONE, Vol 6, Iss 2, p e16779 (2011)

One-third of the world population is infected with Mycobacterium tuberculosis and multi-drug resistant strains are rapidly evolving. The noticeable absence of a whole organism high-throughput screening system for studying the progression of tuberculo

Externí odkaz: https://doaj.org/article/f48d3188535341409455dd502ae3be14

Deep learning image recognition enables efficient genome editing in zebrafish by automated injections

Autor: Alexander D. Crawford, Herman P. Spaink, Ursula Heins-Marroquin, Maria Lorena Cordero-Maldonado, Annemarie H. Meijer, Jan de Sonneville, Simon Perathoner, Kees-Jan van der Kolk, Ralf Boland

Publikováno v: PLOS One, 14(1), e0202377
PLoS ONE, Vol 14, Iss 1, p e0202377 (2019)
PLoS ONE. San Franscisco, CA: Public Library of Science (2019).
PLoS ONE
PLOS One

One of the most popular techniques in zebrafish research is microinjection, as it is a rapid and efficient way to genetically manipulate early developing embryos, and to introduce microbes or tracers at larval stages.Here we demonstrate the developme

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::17ea806a90f01e0ee3aedc55921b8ef6
https://doi.org/10.1371/journal.pone.0202377

Robotic injection of zebrafish embryos for high-throughput screening in disease models

Autor: Wouter J. Veneman, Wietske van der Ent, B. Ewa Snaar-Jagalska, William P.J. Leenders, Herman P. Spaink, Annemarie H. Meijer, Hans J. Jansen, Chao Cui, Ron P. Dirks, Malgorzata Wiweger, Jan de Sonneville, Rubén Marín-Juez, Anita Ordas, Vincenzo Torraca

Publikováno v: Methods; Vol 62
Methods, 62(3), 246-254
Methods: a Journal for Human Science, 62, 3, pp. 246-54
Methods
Methods: a Journal for Human Science, 62, 246-54

Item does not contain fulltext The increasing use of zebrafish larvae for biomedical research applications is resulting in versatile models for a variety of human diseases. These models exploit the optical transparency of zebrafish larvae and the ava

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26f069df2b415047ff1c7833335f14d3

Automated microinjection of cell-polymer suspensions in 3D ECM scaffolds for high-throughput quantitative cancer invasion screens

Autor: Jiangling Xiong, Veerander P. S. Ghotra, Erik H.J. Danen, Leo S. Price, Herman H. Spaink, Jan de Sonneville, Hoa Truong, Pancras C.W. Hogendoorn, Bob van de Water

Publikováno v: Biomaterials; Vol 33
Biomaterials, 33(1), 181-188

Cell spheroids (CS) embedded in 3D extracellular matrix (ECM) serve as in vitro mimics for multicellular structures in vivo. Such cultures, started either from spontaneous cell aggregates or single cells dispersed in a gel are time consuming, applica

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2ac7dcf2bbd3b0b91cac48853feb9927
https://doi.org/10.1016/j.biomaterials.2011.09.049