Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Jamin B. Hein"'
Autor:
Jamin B Hein, Hieu T Nguyen, Dimitriya H Garvanska, Isha Nasa, Thomas Kruse, Yinnian Feng, Blanca Lopez Mendez, Norman Davey, Arminja N Kettenbach, Polly M Fordyce, Jakob Nilsson
Publikováno v:
Molecular Systems Biology, Vol 19, Iss 12, Pp n/a-n/a (2023)
Abstract Phosphoprotein phosphatases (PPPs) regulate major signaling pathways, but the determinants of phosphatase specificity are poorly understood. This is because methods to investigate this at scale are lacking. Here, we develop a novel in vitro
Externí odkaz:
https://doaj.org/article/2b7f9cdcf70344a5bb703577c2b23118
Publikováno v:
ACS Measurement Science Au, Vol 1, Iss 2, Pp 56-64 (2021)
Externí odkaz:
https://doaj.org/article/4da49527d148411098198e887011a55d
Autor:
Jamin B. Hein, Hieu T. Nguyen, Dimitriya H. Garvanska, Isha Nasa, Yinnian Feng, Blanca Lopez Mendez, Norman Davey, Arminja N Kettenbach, Polly M. Fordyce, Jakob Nilsson
Phosphoprotein phosphatases (PPPs) dephosphorylate Serine (Ser)/Threonine (Thr) residues to regulate major signaling pathways and cellular transitions. Despite the central role of PPPs the substrates in most cellular processes and the determinants of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4a976596080a1b5a789e397db127764c
https://doi.org/10.1101/2023.05.14.540683
https://doi.org/10.1101/2023.05.14.540683
Autor:
Jamin B. Hein, Jakob Nilsson
Publikováno v:
Nature Communications, Vol 7, Iss 1, Pp 1-10 (2016)
The Anaphase Promoting Complex/Cyclosome (APC/C) and its co-activator Cdc20 regulate mitotic progression, but both are also present in interphase. Here Hein and Nilsson show that Cyclin A2–CDK2 phosphorylates Cdc20 to inhibit APC/C–Cdc20 activity
Externí odkaz:
https://doaj.org/article/3c229f6b05174cce8ad33f439aa30a13
Publikováno v:
The Journal of Cell Biology
Tight control of Cdc20 activity is required for proper cell division. Here, it is shown that the BubR1 checkpoint protein integrates both Cdc20 inhibition and activation. PP2A-B56 bound to BubR1 removes Cdc20 inhibitory phosphorylation, allowing for
Publikováno v:
ACS Measurement Science Au, Vol 1, Iss 2, Pp 56-64 (2021)
ACS Meas Sci Au
Hein, J B, Cyert, M S & Fordyce, P M 2021, ' MRBLE-pep Measurements Reveal Accurate Binding Affinities for B56, a PP2A Regulatory Subunit ', ACS Measurement Science Au, vol. 1, no. 2, pp. 56-64 . https://doi.org/10.1021/acsmeasuresciau.1c00008
ACS Meas Sci Au
Hein, J B, Cyert, M S & Fordyce, P M 2021, ' MRBLE-pep Measurements Reveal Accurate Binding Affinities for B56, a PP2A Regulatory Subunit ', ACS Measurement Science Au, vol. 1, no. 2, pp. 56-64 . https://doi.org/10.1021/acsmeasuresciau.1c00008
Signal transduction pathways rely on dynamic interactions between protein globular domains and short linear motifs (SLiMs). The weak affinities of these interactions are essential to allow fast rewiring of signaling pathways and downstream responses,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5ea9f55b7474dd5b8ab8152207a1ffca
https://doi.org/10.1101/2020.12.16.423088
https://doi.org/10.1101/2020.12.16.423088
Publikováno v:
Microsystems & Nanoengineering
Feng, Y, White, A K, Hein, J B, Appel, E A & Fordyce, P M 2020, ' MRBLES 2.0 : High-throughput generation of chemically functionalized spectrally and magnetically encoded hydrogel beads using a simple single-layer microfluidic device ', Microsystems and Nanoengineering, vol. 6, 109 . https://doi.org/10.1038/s41378-020-00220-3
Feng, Y, White, A K, Hein, J B, Appel, E A & Fordyce, P M 2020, ' MRBLES 2.0 : High-throughput generation of chemically functionalized spectrally and magnetically encoded hydrogel beads using a simple single-layer microfluidic device ', Microsystems and Nanoengineering, vol. 6, 109 . https://doi.org/10.1038/s41378-020-00220-3
The widespread adoption of bead-based multiplexed bioassays requires the ability to easily synthesize encoded microspheres and conjugate analytes of interest to their surface. Here, we present a simple method (MRBLEs 2.0) for the efficient high-throu
Widespread adoption of bead-based multiplexed bioassays requires the ability to easily synthesize encoded microspheres and conjugate analytes of interest to their surface. Here, we present a simple method (MRBLEs 2.0) for efficient high-throughput ge
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ffab6ef8d263b10cd7327916672d945b
https://doi.org/10.1101/2020.06.22.166074
https://doi.org/10.1101/2020.06.22.166074
Autor:
Jacob Samsøe-Petersen, Hanna Sofia Pena, Jeanette Schwarz, Jakob Nilsson, Blanca López-Méndez, Isha Nasa, Thomas Kruse, Jamin B Hein, Sebastian Gnosa, Marie Kveiborg, Hieu T Nguyen, Emil Peter Thrane Hertz, Denise Nikodemus, Dimitriya H Garvanska, Arminja N. Kettenbach
Publikováno v:
Kruse, T, Gnosa, S P, Nasa, I, Garvanska, D H, Hein, J B, Nguyen, H, Samsøe-Petersen, J, Lopez-Mendez, B, Hertz, E P T, Schwarz, J, Pena, H S, Nikodemus, D, Kveiborg, M, Kettenbach, A N & Nilsson, J 2020, ' Mechanisms of site-specific dephosphorylation and kinase opposition imposed by PP2A regulatory subunits ', EMBO Journal, vol. 39, e103695 . https://doi.org/10.15252/embj.2019103695
The EMBO Journal
The EMBO Journal
PP2A is an essential protein phosphatase that regulates most cellular processes through the formation of holoenzymes containing distinct regulatory B‐subunits. Only a limited number of PP2A‐regulated phosphorylation sites are known. This hampers
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1f8c28d1fd1961a7d7b87251890f6369
https://curis.ku.dk/ws/files/245322909/embj.2019103695.pdf
https://curis.ku.dk/ws/files/245322909/embj.2019103695.pdf
Publikováno v:
Bio Protoc
Every living cell relies on signal transduction pathways comprised of protein-protein interactions (PPIs). In many cases, these PPIs are between a folded protein domain and a short linear motif (SLiM) within an unstructured region of a protein. As a