Anticoagulants Influence the Performance of In Vitro Assays Intended for Characterization of Nanotechnology-Based Formulations

Autor: Edward Cedrone, Barry W. Neun, Jamie Rodriguez, Alison Vermilya, Jeffrey D. Clogston, Scott E. McNeil, Yechezkel Barenholz, Janos Szebeni, Marina A. Dobrovolskaia

Publikováno v: Molecules, Vol 23, Iss 1, p 12 (2017)

The preclinical safety assessment of novel nanotechnology-based drug products frequently relies on in vitro assays, especially during the early stages of product development, due to the limited quantities of nanomaterials available for such studies.

Externí odkaz: https://doaj.org/article/7af0fd1578c248ba9013c602b4599551

Anticoagulants Influence the Performance of In Vitro Assays Intended for Characterization of Nanotechnology-Based Formulations

Autor: Barry W. Neun, Edward Cedrone, Janos Szebeni, Scott E. McNeil, Marina A. Dobrovolskaia, Jamie Rodriguez, Alison Vermilya, Yechezkel Barenholz, Jeffrey D. Clogston

Publikováno v: Molecules; Volume 23; Issue 1; Pages: 12
Molecules, Vol 23, Iss 1, p 12 (2017)
Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

The preclinical safety assessment of novel nanotechnology-based drug products frequently relies on in vitro assays, especially during the early stages of product development, due to the limited quantities of nanomaterials available for such studies.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d2cd57753be09b6fde751f01aefb1569

In Vitro Analysis of Nanoparticle Effects on the Zymosan Uptake by Phagocytic Cells

Autor: Timothy M. Potter, Jamie Rodriguez, Barry W. Neun, Edward Cedrone, Anna Ilinskaya, Sarah L. Skoczen, Marina A. Dobrovolskaia

Publikováno v: Methods in Molecular Biology ISBN: 9781493973507

This chapter provides a protocol for analysis of nanoparticle effects on the function of phagocytic cells. The protocol relies on luminol chemiluminescence to detect zymosan uptake. Zymosan is an yeast particle which is typically eliminated by phagoc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::9dd5ac10e3dd34a8ac1e8b95db1a2285
https://doi.org/10.1007/978-1-4939-7352-1_11

Autophagy Monitoring Assay II: Imaging Autophagy Induction in LLC-PK1 Cells Using GFP-LC3 Protein Fusion Construct

Autor: Krishna P. Kota, Stephan T. Stern, Pavan P. Adiseshaiah, Sarah L. Skoczen, Timothy M. Potter, Jamie Rodriguez

Publikováno v: Methods in Molecular Biology ISBN: 9781493973507

Autophagy is a catabolic process involved in the degradation and recycling of long-lived proteins and damaged organelles for maintenance of cellular homeostasis, and it has also been proposed as a type II cell death pathway. The cytoplasmic component

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::0d19c463ddeda8ec6cf1467691335a0f
https://doi.org/10.1007/978-1-4939-7352-1_18

Wavefront-based PSF estimation

Autor: Jamie Rodriguez, A. Roodman, Christopher P. Davis

Publikováno v: SPIE Proceedings.

A new method to estimate the Point Spread Function from measurements of optical wavefront is described. We apply this method to images taken with the Dark Energy Camera during the Science Verification period of the Dark Energy Survey, and compare our

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::edb4656abb4b9a9fb98585d2c8cc6d5c
https://doi.org/10.1117/12.2233366