Zobrazeno 1 - 10 of 21 pro vyhledávání: '"James W. Bryson"'
1
Expanding and understanding the CRISPR toolbox for Bacillus subtilis with MAD7 and dMAD7
Autor: James W. Bryson, Franck Escalettes, Marcus A. Price, Susan J. Rosser, Rita Cruz
Publikováno v: Price, M A, Cruz, R, Bryson, J, Escalettes, F & Rosser, S J 2020, ' Expanding and understanding the CRISPR toolbox for Bacillus subtilis with MAD7 and dMAD7 ', Biotechnology and Bioengineering . https://doi.org/10.1002/bit.27312
The CRISPR‐Cas9 system has become increasingly popular for genome engineering across all fields of biological research, including in the Gram‐positive model organism Bacillus subtilis. A major draw‐back for the commercial use of Cas9 is the IP
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9ad0ffb4dd3c1741bbc5ecd70f90f059
https://doi.org/10.1002/bit.27312
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2
Multiplexed activation in mammalian cells using a split-intein CRISPR/Cas12a based synthetic transcription factor
Autor: James W Bryson, Jamie Y Auxillos, Susan J Rosser
Publikováno v: Bryson, J, Auxillos, J & Rosser, S J 2021, ' Multiplexed activation in mammalian cells using a split-intein CRISPR/Cas12a based synthetic transcription factor ', Nucleic Acids Research . https://doi.org/10.1093/nar/gkab1191
Nucleic Acids Research
The adoption of CRISPR systems for the generation of synthetic transcription factors has greatly simplified the process for upregulating endogenous gene expression, with a plethora of applications in cell biology, bioproduction and cell reprogramming
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::77f455117614fda01ed3f81aa348a11d
https://hdl.handle.net/20.500.11820/5ae5fad5-a17c-46c4-a655-59bc3c4b0469
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3
Multiplexed activation in mammalian cells using dFnCas12a-VPR
Autor: Susan J. Rosser, Jamie Auxillos, James W. Bryson
The adoption of CRISPR systems for the generation of synthetic transcription factors has greatly simplified the process for upregulating endogenous gene expression, with a plethora of applications in cell biology, bioproduction and cell reprogramming
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::071208024179b86eb5970a978a3912c6
https://doi.org/10.1101/2021.01.31.429049
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4
De novo design of heterotrimeric coiled coils
Autor: William F. DeGrado, Angela Lombardi, James W. Bryson
Publikováno v: Biopolymers. 40:495-504
The three-helix bundle is a common structural motif among natural proteins. It has been observed in numerous important proteins, such as fibrinogen, laminin, spectrin, dystrofin, hemagglutinin, and mannose binding proteins. The three-helix bundle is
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::b8dbf0c154a10f98194f82a7968969f5
https://doi.org/10.1002/(sici)1097-0282(1996)40:5<495::aid-bip7>3.0.co
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5
Mercury-199 NMR of the Metal Receptor Site in MerR and Its Protein-DNA Complex
Autor: Lisa M. Utschig, Thomas V. O'Halloran, James W. Bryson
Publikováno v: Science. 268:380-385
Structural insights have been provided by mercury-199 nuclear magnetic resonance (NMR) into the metal receptor site of the MerR metalloregulatory protein alone and in a complex with the regulatory target, DNA. The one- and two-dimensional NMR data ar
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4827029e3295213a8c7ec5a0890ce0e8
https://doi.org/10.1126/science.7716541
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7
Optimal Flux Map Generation Through Parameter Estimation Techniques
Autor: James W. Bryson, Jeré A. Hassberger, John C. Lee
Publikováno v: Nuclear Science and Engineering. 114:238-251
Two methods are presented for optimally calculating spatial distributions of neutron flux in a nuclear reactor core. Both techniques, Kalman filtering and maximum likelihood estimation, simultaneou...
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::515db7e64fcf4ae868a6b5a41295b2fa
https://doi.org/10.13182/nse93-a24037
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9
UVRR Spectroscopy and Vibrational Analysis of Mercury Thiolate Compounds Resembling d(10) Metal Binding Sites in Proteins
Autor: James W. Bryson, Mária Vargek, Thomas G. Spiro, Gerhard Fleissner, Thomas V. O'Halloran, Pawel M. Kozlowski
Publikováno v: Inorganic chemistry. 38(15)
Raman, ultraviolet resonance Raman (UVRR) and far-IR spectra are reported for the mercury-cysteamine complex, Hg(SCH(2)CH(2)NH(2))(2). Band assignments are made for Hg(SCH(2)CH(2)NH(2))(2), and also for [Hg(SBu(t))(3)](-) and [Hg(SMe)(3)](-) on the b
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0a8327b895b10498974b5bd6453dfa84
https://pubmed.ncbi.nlm.nih.gov/11671099
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10
Design of three-dimensional domain-swapped dimers and fibrous oligomers
Autor: David Eisenberg, James W. Bryson, Mari Gingery, Giovanna Ghirlanda, William F. DeGrado, N.L. Ogihara
Publikováno v: Proceedings of the National Academy of Sciences of the United States of America. 98(4)
Three-dimensional (3D) domain-swapped proteins are intermolecularly folded analogs of monomeric proteins; both are stabilized by the identical interactions, but the individual domains interact intramolecularly in monomeric proteins, whereas they form
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3be31d94b4b53ad6d62d7814097f039a
https://pubmed.ncbi.nlm.nih.gov/11171963
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