Zobrazeno 1 - 10
of 80
pro vyhledávání: '"James W. Bodley"'
Autor:
Sulekha Rao, James W. Bodley
Publikováno v:
Protein Expression and Purification. 8:91-96
Protein synthesis elongation factor 2 (EF-2), a member of the G protein superfamily, catalyzes the movement of the ribosome along mRNA in a reaction driven by the hydrolysis of GTP. In the present study, we have expressed, purified, and characterized
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::749f72164952df9526fc31332e3d8670
https://doi.org/10.1006/prep.1996.0078
https://doi.org/10.1006/prep.1996.0078
Publikováno v:
Gene. 148:143-147
The single polypeptide chain of elongation factor 2 (EF-2) is encoded by two Saccharomyces cerevisiae genes ( EFT1 and EFT2 ) with unique flanking sequences. One gene is necessary and either is sufficient for cell viability. In the present work, we h
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::40ede1a5bbbae3fefe8c6c29ea9efdc4
https://doi.org/10.1016/0378-1119(94)90248-8
https://doi.org/10.1016/0378-1119(94)90248-8
Publikováno v:
Journal of Biological Chemistry. 269:9084-9089
Functional domains of the bacteriophage phi 29 prohead RNA (pRNA) that are essential for in vitro packaging of DNA-gp3 into the prohead were mapped using pRNA mutants. Oligonucleotide-directed mutant pRNAs were produced that contained deletions and s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b1ebefc9b73a91e01e9c7e541131c3ed
https://doi.org/10.1016/s0021-9258(17)37080-1
https://doi.org/10.1016/s0021-9258(17)37080-1
Publikováno v:
Journal of Biological Chemistry. 269:5157-5162
The small prohead RNA (pRNA) of the Bacillus subtilis bacteriophage phi 29 is essential for ATP-dependent packaging of viral DNA. The 174-, 124-, and 120-residue forms of pRNA produced in vitro using T7 RNA polymerase were equivalent in prohead bindi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::07f21d22fad44fd90384d7c60f0fd8a4
https://doi.org/10.1016/s0021-9258(17)37669-x
https://doi.org/10.1016/s0021-9258(17)37669-x
Autor:
W B Gleason, Dennis M. Livingston, L D Phan, John P. Perentesis, James W. Bodley, David C. LaPorte
Publikováno v:
Journal of Biological Chemistry. 267:1190-1197
The elongation factor 2 (EF-2) genes of the yeast Saccharomyces cerevisiae have been cloned and characterized with the ultimate goal of gaining a better understanding of the mechanism and control of protein synthesis. Two genes (EFT1 and EFT2) were i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7a252a0a62a2476bed74f5e1bea77014
https://doi.org/10.1016/s0021-9258(18)48413-x
https://doi.org/10.1016/s0021-9258(18)48413-x
Autor:
James W. Bodley, Stephen P. Miller
Publikováno v:
Nucleic Acids Research. 19:1657-1660
The translocation reaction catalyzed by elongation factor G (EF-G) is inhibited either by alpha-sarcin cleavage of 23S rRNA or by the binding of thiostrepton to the E. coli ribosome. Here we show that the transitory binding of EF-G and GDP to the rib
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f962999634a97ab6ae5d13fc22c3e80f
https://doi.org/10.1093/nar/19.7.1657
https://doi.org/10.1093/nar/19.7.1657
Autor:
Jesdawan Wichitwechkarn, James W. Bodley, B E Reilly, Suzanne Bailey, Darrin P. Johnson, Dwight L. Anderson
Publikováno v:
Journal of Biological Chemistry. 265:22365-22370
An unusual RNA molecule encoded by the Bacillus subtilis bacteriophage phi 29 is a structural component of the viral prohead and is required for the ATP-dependent packaging of DNA. Here we report a model of secondary structure for this prohead RNA de
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f2505de5d29a9448f50aa110e027c613
https://doi.org/10.1016/s0021-9258(18)45714-6
https://doi.org/10.1016/s0021-9258(18)45714-6
Autor:
James W. Bodley
Publikováno v:
Science. 250:832-838
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::41af1717170f8bed7d363556782bcae7
https://doi.org/10.1126/science.2237435
https://doi.org/10.1126/science.2237435
Autor:
James W. Bodley, Dwight L. Anderson
Publikováno v:
Journal of Structural Biology. 104:70-74
A novel bacteriophage φ29 RNA of 174 nucleotides is essential for the in vitro packaging of the DNA-terminal protein complex into proheads. The RNA, bound to the prohead portal vertex (connector), participates in assembly and function of the DNA tra
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2374bbfbcf7083938d3dcf8962fa1cab
https://doi.org/10.1016/1047-8477(90)90059-l
https://doi.org/10.1016/1047-8477(90)90059-l
Publikováno v:
Yeast (Chichester, England). 8(8)
A genetal method is described for screening Saccharomyces cerevisiae colonies for the intracellular expression of native proteins. Colonies are replicated onto nitrocellulose membranes and yeast walls are removed enzymatically. The resulting spheropl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5ce98fe14ce73ac3bd4115518dbed801
https://pubmed.ncbi.nlm.nih.gov/1441742
https://pubmed.ncbi.nlm.nih.gov/1441742