Zobrazeno 1 - 10
of 59
pro vyhledávání: '"James W Brooks"'
Autor:
Athmaram Thimmasandra Narayanappa, Sam Mwilu, Stacy Holdread, Kimesha Hammett, George Bu, Elizabeth C Dodson, James W Brooks
Publikováno v:
BioTechniques, Vol 67, Iss 3, Pp 98-109 (2019)
Poloxamers are water-soluble polymers that are widely used in cell culture bioprocessing to protect cells against shearing forces. Use of poor-quality poloxamers may lead to a drastic reduction in cell growth, viabilities and productivities in cell c
Externí odkaz:
https://doaj.org/article/1443f9777989478592f0c268739c7c90
Publikováno v:
Biotechnology Reports, Vol 1, Iss C, Pp 22-26 (2014)
In biopharmaceutical production, the optimization of cell culture media and supplementation is a vital element of process development. Optimization is usually achieved through the screening of multiple media, feed and feeding strategies. However, mos
Externí odkaz:
https://doaj.org/article/b56b2a6c504140de8a9defd2c3fe3b6f
Autor:
James W. Brooks, George Bu, Kimesha Hammett, Sam Mwilu, Athmaram Thimmasandra Narayanappa, Stacy Holdread, Elizabeth C Dodson
Publikováno v:
BioTechniques. 67(3)
Poloxamers are water-soluble polymers that are widely used in cell culture bioprocessing to protect cells against shearing forces. Use of poor-quality poloxamers may lead to a drastic reduction in cell growth, viabilities and productivities in cell c
Publikováno v:
Biotechnology Reports
Biotechnology Reports, Vol 1, Iss C, Pp 22-26 (2014)
Biotechnology Reports, Vol 1, Iss C, Pp 22-26 (2014)
Highlights • CHO cell cultures in 24DW plates exhibit similar kinetics in growth, viability and protein production to those cultured in shake flasks. • Well–well and plate–plate variation in growth and protein production of CHO cells is ≤10
Autor:
Hyangkyu Lee, Shana Marmon, Michael P. Lisanti, James W. Brooks, Roberto Campos-Gonzalez, Xiaobo Wang, Franco Capozza, Federica Sotgia
Publikováno v:
Biochemistry. 43:13694-13706
Caveolin-2 is an accessory molecule and the binding partner of caveolin-1. Previously, we showed that c-Src expression leads to the tyrosine phosphorylation of Cav-2 at position 19. To further investigate the tyrosine phosphorylation of Cav-2, we hav
Requirement for CD40 Ligand, CD4 + T Cells, and B Cells in an Infectious Mononucleosis-Like Syndrome
Autor:
Rhonda D. Cardin, Ann Marie Hamilton-Easton, Jan Pravsgaard Christensen, James W. Brooks, Charles L. Hardy, Peter C. Doherty
Publikováno v:
Journal of Virology. 73:9650-9654
Respiratory challenge with the murine gammaherpesvirus 68 (γHV-68) results in productive infection of the lung, the establishment of latency in B lymphocytes and other cell types, transient splenomegaly, and prolonged clonal expansion of activated C
Autor:
James W. Brooks, Malcolm K. Brenner, James N. Ihle, Ralph A. Tripp, Peter C. Doherty, Jan M. van Deursen, Michael Brown, Tetsuya Nosaka
Publikováno v:
Blood. 94:1906-1914
Expansion of early lymphoid progenitors requires interleukin-7 (IL-7), which functions through gamma(c)-mediated receptor activation of Jak3. Jak3 deficiency is a cause of severe combined immunodeficiency (SCID) in humans and mice. IL-3 activates man
Publikováno v:
The Journal of Experimental Medicine
A unique experimental model has been developed for dissecting the integrity of CD8+ T cell-mediated immunity to a persistent gammaherpesvirus under conditions of CD4+ T cell deficiency. Respiratory challenge of major histocompatibility complex class
Autor:
James W. Brooks, Kaci Conaway
Publikováno v:
Genetic Engineering & Biotechnology News. 36:20-20
Autor:
Christopher E. Desch, William J. Frable, James W. Brooks, Barbara L. Stanfield, Celeste N. Powers
Publikováno v:
Diagnostic Cytopathology. 7:423-426
A case of primary chondrosarcoma of the lung diagnosed by fine-needle aspiration biopsy (FNAB) cytology in a 78-yr-old male is presented. A mass detected on chest x-ray and defined by CT scan was subjected to a preoperative percutaneous fine-needle a