Zobrazeno 1 - 10
of 10
pro vyhledávání: '"James T. Heads"'
Publikováno v:
mAbs, Vol 14, Iss 1 (2022)
The propensity for some monoclonal antibodies (mAbs) to aggregate at physiological and manufacturing pH values can prevent their use as therapeutic molecules or delay time to market. Consequently, developability assessments are essential to select op
Externí odkaz:
https://doaj.org/article/ae4bb49c558444769fdeb77ccc96bfe5
Autor:
Alastair D. G. Lawson, Shauna West, Richard Lamb, James T. Heads, Ruodan Nan, Ralph Adams, Peter Charles Elliott, Alison Turner, Sebastian Kelm, Kerry Louise Tyson, Sarfaraj Topia
Publikováno v:
Protein Engineering, Design and Selection
Native state aggregation is an important concern in the development of therapeutic antibodies. Enhanced knowledge of mAb native state aggregation mechanisms would permit sequence-based selection and design of therapeutic mAbs with improved developabi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::987ae6d7f0f396ff33733534054afdf1
https://doi.org/10.1093/protein/gzz046
https://doi.org/10.1093/protein/gzz046
Autor:
Brian J. Sutton, Tom Ceska, Anna M. Davies, Alkistis N. Mitropoulou, James T. Heads, James M. McDonnell, Andrew J. Beavil, Alistair James Henry
Publikováno v:
Acta Crystallographica. Section F, Structural Biology Communications
The omalizumab Fab was engineered to disrupt recurring crystal packing interactions in Fab crystal structures; this led to the eventual structure determination of an omalizumab-derived Fab in complex with its target, IgE-Fc.
Immunoglobulin E (Ig
Immunoglobulin E (Ig
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::293c4b997cabcdd9f87b8598458f3600
https://pubmed.ncbi.nlm.nih.gov/32133997
https://pubmed.ncbi.nlm.nih.gov/32133997
Autor:
Alastair D. G. Lawson, Ralph Adams, Joanne E. Compson, Tim Kopotsha, Michael Airey, Hanna Hailu, David Paul Humphreys, Oliver Zaccheo, Kaushik Sarkar, Mark Jairaj, Wild Gavin Barry, Andrew M. Ventom, Sarah L. Dugdale, Louis Christodoulou, Emma Dave, Sarah Malcolm, Diane Marshall, Alison Turner, Bruce Carrington, James T. Heads, Miren Zamacona, Sam Philip Heywood
Publikováno v:
mAbs
An antibody format, termed Fab-dsFv, has been designed for clinical indications that require monovalent target binding in the absence of direct Fc receptor (FcR) binding while retaining substantial serum presence. The variable fragment (Fv) domain of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7a7a3913eb730ba743647c72b63a2c22
http://europepmc.org/articles/PMC5058625
http://europepmc.org/articles/PMC5058625
Autor:
Bernie Sweeney, Andy Ventom, Paul Edward Stephens, Alan J. Dickson, James T. Heads, Kaushik Sarkar, Hanna Hailu, Catherine Page, Katharine Cain, Shirley Jane Peters
Publikováno v:
Biotechnology Progress. 29:697-706
Transient gene expression (TGE) systems currently provide rapid and scalable (up to 100 L) methods for generating multigram quantities of recombinant heterologous proteins. Product titers of up to 1 g/L have been demonstrated in HEK293 cells but repo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7bfc6bf5b36cdd6d23504a78db71f446
https://doi.org/10.1002/btpr.1693
https://doi.org/10.1002/btpr.1693
Autor:
Amanda S. Cross, Brendon MacKenzie, Louis Christodoulou, James T. Heads, Carl Brendan Doyle, Matthew Page, Hanna Hailu, Karine Jeannine Madeleine Hervé, Mark Ellis, Sarah L. Dugdale, Victoria O’Dowd, Nicola L. Davies, Alison Turner, Daniel John Lightwood, Adrian Moore, David Edward Ormonde Knight, Marco Kriek, Kaushik Sarkar, Mark Jairaj, David P. Humphreys, Matthew Cox, Kerry Louise Tyson, Joanne E. Compson, Amanda K. F. Oxbrow
Publikováno v:
Clinical and Vaccine Immunology. 20:377-390
Clostridium difficile infections are a major cause of antibiotic-associated diarrhea in hospital and care facility patients. In spite of the availability of effective antibiotic treatments, C. difficile infection (CDI) is still a major cause of patie
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::502a01b494632943b9983cbcfab8a7ed
https://doi.org/10.1128/cvi.00625-12
https://doi.org/10.1128/cvi.00625-12
Autor:
Lena E. D’Hooghe, Alastair D. G. Lawson, James T. Heads, Ralph Adams, Alistair James Henry, Andrew George Popplewell, Matt Page, David P. Humphreys
Publikováno v:
Protein Science. 21:1315-1322
The stability of therapeutic antibodies is a prime pharmaceutical concern. In this work we examined thermal stability differences between human IgG1 and IgG4 Fab domains containing the same variable regions using the thermofluor assay. It was found t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9146cda652f223da5de20f12a7c8ac7e
https://doi.org/10.1002/pro.2118
https://doi.org/10.1002/pro.2118
Autor:
Eve E. Weatherill, James T. Heads, Ralph Adams, Katharine Cain, Joanne E. Compson, David Paul Humphreys, Sam Philip Heywood
Publikováno v:
Protein engineering, designselection : PEDS. 25(7)
Engineered introduction of interface interchain disulphide bonds is perceived to be a simple method to increase the stability of single chain Fv (scFv). Six disulphide bond locations have been cited within the literature but the potential for the bro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3af001ff7b9873ef196d772893312080
https://pubmed.ncbi.nlm.nih.gov/22586154
https://pubmed.ncbi.nlm.nih.gov/22586154
Autor:
James T, Heads, Ralph, Adams, Lena E, D'Hooghe, Matt J T, Page, David P, Humphreys, Andrew G, Popplewell, Alastair D, Lawson, Alistair J, Henry
Publikováno v:
Protein science : a publication of the Protein Society. 21(9)
The stability of therapeutic antibodies is a prime pharmaceutical concern. In this work we examined thermal stability differences between human IgG1 and IgG4 Fab domains containing the same variable regions using the thermofluor assay. It was found t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::6b91594f8c05bc3a6edbcc356b56cdae
https://pubmed.ncbi.nlm.nih.gov/22761163
https://pubmed.ncbi.nlm.nih.gov/22761163
Autor:
John OʼHara, Andrew Nesbitt, Alistair James Henry, Sam Philip Heywood, Thomas Allen Ceska, Alison Turner, James T. Heads, Bryan Smith
Publikováno v:
American Journal of Gastroenterology. 103:S430
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::49d20b3354cd32314a7edc4db9c6fd5c
https://doi.org/10.14309/00000434-200809001-01102
https://doi.org/10.14309/00000434-200809001-01102