Zobrazeno 1 - 10
of 138
pro vyhledávání: '"James T Stivers"'
Autor:
Mesfin Meshesha, Alexandre Esadze, Junru Cui, Natela Churgulia, Sushil Kumar Sahu, James T Stivers
Publikováno v:
PLoS ONE, Vol 15, Iss 7, p e0235012 (2020)
Non-dividing cells of the myeloid lineage such as monocytes and macrophages are target cells of HIV that have low dNTP pool concentrations and elevated levels of dUTP, which leads to frequent incorporation of dUMP opposite to A during reverse transcr
Externí odkaz:
https://doaj.org/article/42026a354a3e4c28bf5b089f30c9b653
Autor:
Erik C Hansen, Monica Ransom, Jay R Hesselberth, Nina N Hosmane, Adam A Capoferri, Katherine M Bruner, Ross A Pollack, Hao Zhang, Michael Bradley Drummond, Janet M Siliciano, Robert Siliciano, James T Stivers
Publikováno v:
eLife, Vol 5 (2016)
We report that a major subpopulation of monocyte-derived macrophages (MDMs) contains high levels of dUTP, which is incorporated into HIV-1 DNA during reverse transcription (U/A pairs), resulting in pre-integration restriction and post-integration mut
Externí odkaz:
https://doaj.org/article/db3a368d8fbf4bbfb556874b4bc89f4b
Autor:
Benjamin Orris, Kevin W. Huynh, Mark Ammirati, Seungil Han, Ben Bolaños, Jason Carmody, Matthew D. Petroski, Benedikt Bosbach, David J. Shields, James T. Stivers
Publikováno v:
Nucleic Acids Research. 50:7545-7559
SAM and HD domain containing deoxynucleoside triphosphate triphosphohydrolase 1 (SAMHD1) is driven into its activated tetramer form by binding of GTP activator and dNTP activators/substrates. In addition, the inactive monomeric and dimeric forms of t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a1b94213327a9411effec66bd325d4c2
https://doi.org/10.1093/nar/gkac573
https://doi.org/10.1093/nar/gkac573
Autor:
Matthew Egleston, Linghao Dong, A. Hasan Howlader, Shridhar Bhat, Benjamin Orris, Mario A. Bianchet, Marc M. Greenberg, James T. Stivers
Publikováno v:
ACS Chemical Biology.
This upload contains all of the experimental data and analysis files pertaining to the accepted manuscript.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e4e21ce5cbf4441deeb2e793ac8f378d
https://doi.org/10.1021/acschembio.3c00118
https://doi.org/10.1021/acschembio.3c00118
Autor:
Junru Cui, James T. Stivers, Anthony S. Gizzi, Matthew Egleston, Benjamin Orris, Eric S. Christenson, Michael DePasquale, Kyle J. Seamon, Ben Ho Park
Publikováno v:
Molecular Pharmacology. 99:412-425
Previous short-hairpin RNA knockdown studies have established that depletion of human uracil DNA glycosylase (hUNG) sensitizes some cell lines to 5-fluorodeoxyuridine (FdU). Here, we selectively inhibit the catalytic activity of hUNG by lentiviral tr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::769bc1ef00d9adc530b4cc371ed8abdb
https://doi.org/10.1124/molpharm.120.000191
https://doi.org/10.1124/molpharm.120.000191
Autor:
Junru Cui, Mesfin Meshesha, Natela Churgulia, Christian Merlo, Edward Fuchs, Jennifer Breakey, Joyce Jones, James T. Stivers
Background Although CD4+ memory T cells are considered the primary latent reservoir for HIV-1, replication competent HIV has been detected in tissue macrophages in both animal and human studies. During in vitro HIV infection, the depleted nucleotide
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b6e67d67b35c87ec64df006ccd1df906
https://doi.org/10.1101/2022.05.03.490432
https://doi.org/10.1101/2022.05.03.490432
Autor:
Junru Cui, Mesfin Meshesha, Alexandre Esadze, Natela Churgulia, Sushil Kumar Sahu, James T. Stivers
Publikováno v:
PLoS ONE
PLoS ONE, Vol 15, Iss 7, p e0235012 (2020)
PLoS ONE, Vol 15, Iss 7, p e0235012 (2020)
Background Non-dividing cells of the myeloid lineage such as monocytes and macrophages are target cells of HIV that have low dNTP pool concentrations and elevated levels of dUTP, which leads to frequent incorporation of dUMP opposite to A during reve
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::225f0f61c084135ce95050e868bd8c06
https://pubmed.ncbi.nlm.nih.gov/32663205
https://pubmed.ncbi.nlm.nih.gov/32663205
Publikováno v:
Nucleic Acids Research
DNA ‘sliding’ by human repair enzymes is considered to be important for DNA damage detection. Here, we transfected uracil-containing DNA duplexes into human cells and measured the probability that nuclear human uracil DNA glycosylase (hUNG2) exci
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f59a30fe51cc39a755fa97d1d2dea8c5
https://doi.org/10.1093/nar/gkx848
https://doi.org/10.1093/nar/gkx848
Publikováno v:
Nucleic Acids Research
dUTP is a close structural congener of dTTP and can be readily incorporated into DNA opposite to adenine during DNA replication leading to non-mutagenic dU/A base pairs (‘uracilation’). We find that dU/A pairs located within DNA transcriptional t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::380e9b06d6b4daba7cf83941d6768036
https://pubmed.ncbi.nlm.nih.gov/30892639
https://pubmed.ncbi.nlm.nih.gov/30892639
Publikováno v:
Cancer Research. 80:1390-1390
Previously reported shRNA knockdown studies have established that expression of the DNA repair enzyme human uracil DNA glycosylase (hUNG2) is protective against 5-flourodeoxyuridine (5FdUrd) cell killing in p53 mutated cancer cell lines, but not in c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::548caf174518de3400e3b8a95f164593
https://doi.org/10.1158/1538-7445.am2020-1390
https://doi.org/10.1158/1538-7445.am2020-1390