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pro vyhledávání: '"James R. Y. Rawson"'
Autor:
William H. Andrews, James R. Y. Rawson
Publikováno v:
Plasmid. 8:148-163
Recombinant plasmids containing restriction endonuclease fragments of chloroplast DNA from the alga Euglena gracilis were expressed in an in vitro DNA-dependent transcription-translation system prepared from E. coli . The polypeptides synthesized in
Autor:
James R. Y. Rawson, Kim P. Dix
Publikováno v:
Current Genetics. 7:265-272
Cloned chloroplast restriction endonuclease DNA fragments were used as hybridization probes to identify the in vivo transcriptional products of the chloroplast genome from the alga Euglena gracilis. Total cellular RNA was size fractionated by electro
Autor:
James R. Y. Rawson, Charles F. Wimpee
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis. 562:192-206
The nuclear genome of pearl millet has been characterized with respect to its size, buoyant density in CsCl equilibrium density gradients, melting temperature, reassociation kinetics and sequence organization. The genome size is 0.22 pg. The mol perc
Publikováno v:
Biochemical Genetics. 20:209-219
A procedure is outlined for purifying DNA from a single plant. A crude organelle pellet consisting of nuclei, chromatin, chloroplasts, and mitochondria is prepared, suspended, and immediately lysed with detergents. The DNA is separated from RNA, prot
Publikováno v:
Plant Physiology. 77:828-832
The light-dependent development of the photosynthetic apparatus in the first leaf of the C(4) plant pearl millet (Pennisetum americanum) was monitored by immunologically determining the concentration of phospho-enolpyruvate carboxylase and ribulose 1
Autor:
Cindy L. Boerma, James R. Y. Rawson
Publikováno v:
Biochemistry. 15:588-592
The fraction of chloroplast DNA transcribed at different stages of chloroplast development in Euglena gracilis was measured by RNA-DNA hybridization. Euglena cells were grown in the dark in a heterotrophic medium to stationary phase and then transfer
Autor:
James R. Y. Rawson, Cindy L. Boerma
Publikováno v:
Biochemical and Biophysical Research Communications. 74:912-918
Chloroplast DNA from Euglena gracilis was separated in an alkaline CsC1 density gradient into a heavy and a light component. The heavy and the light DNA components each contain 18 percent self-complementary nucleotide sequences. Equal masses of the h
Publikováno v:
Biochemistry. 18:5299-5304
Publikováno v:
Plant Physiology. 69:67-71
The nuclear and chloroplast ribosomal DNAs from Euglena were shown to have specific regions of nucleotide sequence homology. The regions of homology were identified by hybridization of restriction endonuclease DNA fragments of cloned chloroplast and
Publikováno v:
Current Genetics. 8:291-297
Chloroplast DNA from pearl millet (Pennisetum americanum) was used to construct recombinant plasmids. These plasmids contained 97 kilobase pairs of unique DNA sequences. The chloroplast DNA fragments in these plasmids were mapped with the restriction