Zobrazeno 1 - 10
of 34
pro vyhledávání: '"James R. Skinner"'
Publikováno v:
Journal of Lipid Research, Vol 53, Iss 5, Pp 1021-1025 (2012)
Fat droplets (FDs) have important roles in cellular energy regulation. Isolating FDs from either cells or tissue continues to be important for studying these organelles. Here, we describe a procedure wherein whole homogenates of cultured cells or tis
Externí odkaz:
https://doaj.org/article/2ead55cdce644fa58516c0c560bf9b12
Autor:
Nathan E. Wolins, Benjamin K. Quaynor, James R. Skinner, Anatoly Tzekov, Changwon Park, Kyunghee Choi, Perry E. Bickel
Publikováno v:
Journal of Lipid Research, Vol 47, Iss 2, Pp 450-460 (2006)
Much knowledge of adipocyte biology has been learned from cell culture models, most notably 3T3-L1 cells. The 3T3-L1 model has several limitations, including the requirement of 2 weeks to generate adipocytes and the waning of adipogenic potential in
Externí odkaz:
https://doaj.org/article/2f364671be38440895fd1771747653dc
The two major products of intestinal triacylglycerol digestion and lipoprotein lipolysis are monoacylglycerols (MAG) and fatty acids. In the gut, these products are taken up by enterocytes and packaged into perilipin-coated cytosolic lipid droplets a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7cb242e320229ed2bad47ee46ef8c076
https://doi.org/10.1101/2021.07.09.451829
https://doi.org/10.1101/2021.07.09.451829
Autor:
James R. Skinner, Trevor M. Shew, Nathan E. Wolins, Nada A. Abumrad, Lydia-Ann L.S. Harris, Terri A. Pietka
Publikováno v:
Diabetes. 64:2757-2768
Perilipin 5 (PLIN5) is a lipid droplet protein and is highly expressed in oxidative tissue. Expression of the PLIN5 gene is regulated by peroxisome proliferator–activated receptor-α, fasting, and exercise. However, the effect of increased muscle P
Publikováno v:
Adipocyte
Perilipin 1, unlike the other perilipins, is thought to be restricted to the fat droplet. We reassessed its cellular distribution using the fat droplet marker CGI-58 in OP9 and 3T3-L1 adipocyte lines and in brown adipose tissue (BAT). As expected, we
Publikováno v:
Journal of Lipid Research, Vol 53, Iss 5, Pp 1021-1025 (2012)
Fat droplets (FDs) have important roles in cellular energy regulation. Isolating FDs from either cells or tissue continues to be important for studying these organelles. Here, we describe a procedure wherein whole homogenates of cultured cells or tis
Autor:
Xiong Su, Nada A. Abumrad, Ondrej Kuda, Richard W. Gross, James R. Skinner, Sung Ho Moon, Christopher M. Jenkins
Publikováno v:
Journal of Biological Chemistry. 286:17785-17795
The scavenger receptor FAT/CD36 contributes to the inflammation associated with diabetes, atherosclerosis, thrombosis, and Alzheimer disease. Underlying mechanisms include CD36 promotion of oxidative stress and its signaling to stress kinases. Here w
Autor:
James R. Skinner, Kyunghee Choi, Nathan E. Wolins, Changwon Park, Perry E. Bickel, Anatoly Tzekov, Benjamin K. Quaynor
Publikováno v:
Journal of Lipid Research, Vol 47, Iss 2, Pp 450-460 (2006)
Much knowledge of adipocyte biology has been learned from cell culture models, most notably 3T3-L1 cells. The 3T3-L1 model has several limitations, including the requirement of 2 weeks to generate adipocytes and the waning of adipogenic potential in
Intracellular fat droplets are large and have a distinct morphology, which makes their imaging at the light level simple and informative. We detail how to image the fat droplet core by metabolic labeling with fluorescent fatty acids or lipophilic flu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::67e88c195b9a98358bcd9eb76318531b
https://europepmc.org/articles/PMC3870878/
https://europepmc.org/articles/PMC3870878/
Autor:
Nada A. Abumrad, Christin M. Lepus, Anatoly Tzekov, James R. Skinner, Danielle M. Schwartz, Trevor M. Shew, Nathan E. Wolins
Publikováno v:
The Journal of biological chemistry. 284(45)
Fatty acid-induced triacylglycerol synthesis produces triacylglycerol droplets with a protein coat that includes perilipin 3/TIP47 and perilipin 4/S3-12. This study addresses the following two questions. Where do lipid droplets emerge, and how are th