Biosensor and machine learning-aided engineering of an amaryllidaceae enzyme

Autor: Simon d’Oelsnitz, Daniel J. Diaz, Wantae Kim, Daniel J. Acosta, Tyler L. Dangerfield, Mason W. Schechter, Matthew B. Minus, James R. Howard, Hannah Do, James M. Loy, Hal S. Alper, Y. Jessie Zhang, Andrew D. Ellington

Publikováno v: Nature Communications, Vol 15, Iss 1, Pp 1-14 (2024)

Abstract A major challenge to achieving industry-scale biomanufacturing of therapeutic alkaloids is the slow process of biocatalyst engineering. Amaryllidaceae alkaloids, such as the Alzheimer’s medication galantamine, are complex plant secondary m

Externí odkaz: https://doaj.org/article/a590be639471443d8f68f7c532e9fc11

Amines as Activating Ligands for Phosphine Palladium(II) Precatalysts: Effect of Amine Ligand Identity on the Catalyst Efficiency

Autor: Jordan K. Pierce, Lindsey D. Hiatt, James R. Howard, Huaiyuan Hu, Fengrui Qu, Kevin H. Shaughnessy

Publikováno v: Organometallics. 41:3861-3871

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::cecf1c3a115dd1797fd89fa4d2a26cdf
https://doi.org/10.1021/acs.organomet.2c00518

Chirality Sensing with UV-visible Absorbance, Fluorescence, and Circular Dichroism Spectroscopy

Autor: James R. Howard, Jongdoo Lim, Sarah R. Moor, Eric V. Anslyn

Publikováno v: Fluorescent Chemosensors ISBN: 9781839163869

Chirality-sensing techniques utilizing UV-visible, fluorescence, and circular dichroism spectroscopy are discussed in detail. The design strategies, applications, and limitations associated with these techniques are considered, as well as relevant hi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3521bcfd9620add0ef991cbb0a31847a
https://doi.org/10.1039/9781839167324-00160

A Colorimetric Method for Quantifying Cis and Trans Alkenes Using an Indicator Displacement Assay

Autor: Gabriel Saucedo, James R. Howard, Chao-Yi Yao, A. Prasanna de Silva, Stephanie A. Valenzuela, Hannah S. N. Crory, Eric V. Anslyn

Publikováno v: Crory, H, Yao, C, De Silva, A (ed.), Anslyn, E, Valenzuela, S, Howard, J & Saucedo, G 2021, ' A Colorimetric Method for Quantifying Cis- and Trans-Alkenes Using an Indicator Displacement Assay ', Angewandte Chemie, pp. 1-6 . https://doi.org/10.1002/ange.202101004
Angew Chem Int Ed Engl

A colorimetric indicator displacement assay (IDA) amenable to high-throughput experimentation was developed to determine the percentage of cis- and trans-alkenes. Using 96-well plates two steps are performed: a reaction plate for dihydroxylation of t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::daa08b8a8240d66b89b7ada153e64ef7
https://doi.org/10.1002/ange.202101004

Degraded cardiomyocytes and dysfunctional energy metabolism; their relationship with canine cardiomyopathies

Autor: James R Howard, Paul N. McMillan

Publikováno v: Mitochondrion. 3:355-363

Tissue sections from hearts of dogs suffering from cardiomyopathies were studied histologically and ultrastructurally. Two types of mitochondrial changes were defined and quantitated. Mitochondrial hypertrophy occurred in cardiomyocytes of middle-age

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3d27ca45d94082ca5347c7b93fc29ed1
https://doi.org/10.1016/j.mito.2004.02.008