Zobrazeno 1 - 10
of 266
pro vyhledávání: '"James R Sellers"'
Autor:
Wen Lu, Margot Lakonishok, Rong Liu, Neil Billington, Ashley Rich, Michael Glotzer, James R Sellers, Vladimir I Gelfand
Publikováno v:
eLife, Vol 9 (2020)
Local accumulation of oskar (osk) mRNA in the Drosophila oocyte determines the posterior pole of the future embryo. Two major cytoskeletal components, microtubules and actin filaments, together with a microtubule motor, kinesin-1, and an actin motor,
Externí odkaz:
https://doaj.org/article/da1f41b7385848a7b9f2c71e6841a8dd
Autor:
Luca Melli, Neil Billington, Sara A Sun, Jonathan E Bird, Attila Nagy, Thomas B Friedman, Yasuharu Takagi, James R Sellers
Publikováno v:
eLife, Vol 7 (2018)
Nonmusclemyosin 2 (NM-2) powers cell motility and tissue morphogenesis by assembling into bipolar filaments that interact with actin. Although the enzymatic properties of purified NM-2 motor fragments have been determined, the emergent properties of
Externí odkaz:
https://doaj.org/article/cb1b9d31e9ba413fa47328cdf4cc9808
Autor:
Krishna Chinthalapudi, Sarah M Heissler, Matthias Preller, James R Sellers, Dietmar J Manstein
Publikováno v:
eLife, Vol 6 (2017)
Despite a generic, highly conserved motor domain, ATP turnover kinetics and their activation by F-actin vary greatly between myosin-2 isoforms. Here, we present a 2.25 Å pre-powerstroke state (ADP⋅VO4) crystal structure of the human nonmuscle myos
Externí odkaz:
https://doaj.org/article/e86762993a7243febaacd1f1f457d1c2
Publikováno v:
eLife, Vol 5 (2016)
Non-muscle cell contractility is critical for tissues to adopt shape changes. Although, the non-muscle myosin II holoenzyme (myosin) is a molecular motor that powers contraction of actin cytoskeleton networks, recent studies have questioned the impor
Externí odkaz:
https://doaj.org/article/eeb71bd4066e4a7fb4e74e61b434937e
Autor:
Joanna Andrecka, Jaime Ortega Arroyo, Yasuharu Takagi, Gabrielle de Wit, Adam Fineberg, Lachlan MacKinnon, Gavin Young, James R Sellers, Philipp Kukura
Publikováno v:
eLife, Vol 4 (2015)
Myosin 5a is a dual-headed molecular motor that transports cargo along actin filaments. By following the motion of individual heads with interferometric scattering microscopy at nm spatial and ms temporal precision we found that the detached head occ
Externí odkaz:
https://doaj.org/article/5296d3698e654e7698cb386bbe418883
Publikováno v:
Frontiers in Physiology, Vol 5 (2014)
Striated muscle is an elegant system for study at many levels. Much has been learned about the mechanism of contraction from studying the mechanical properties of intact and permeabilized (or skinned) muscle fibers. Structural studies using electron
Externí odkaz:
https://doaj.org/article/c582b371350f4945ad305d1b95c8b401
Autor:
Nikolett T Nagy, Saikat Chakraborty, Gábor M Harami, James R Sellers, Takeshi Sakamoto, Mihály Kovács
Publikováno v:
PLoS ONE, Vol 8, Iss 5, p e62640 (2013)
The motor domain of myosin is the core element performing mechanochemical energy transduction. This domain contains the actin and ATP binding sites and the base of the force-transducing lever. Coordinated subdomain movements within the motor are esse
Externí odkaz:
https://doaj.org/article/b396a8f5e8e648d5917cf79fe9a11969
Publikováno v:
PLoS ONE, Vol 8, Iss 9, p e74936 (2013)
How myosin 10, an unconventional myosin, walks processively along actin is still controversial. Here, we used single molecule fluorescence techniques, TIRF and FIONA, to study the motility and the stepping mechanism of dimerized myosin 10 heavy-merom
Externí odkaz:
https://doaj.org/article/f58605a3fd774487906e67d6f1cd78b1
Autor:
Rong Liu, Neil Billington, Yi Yang, Charles Bond, Amy Hong, Verl Siththanandan, Yasuharu Takagi, James R. Sellers
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
Myosin-7a is found in actin bundles, microvilli and stereocilia, and plays conserved roles in hearing and vision. Here the authors identify M7BP, a myosin-7a binding protein that activates and dimerizes myosin-7a, enabling cargo transport and assembl
Externí odkaz:
https://doaj.org/article/82ee430ef28b4669aae273c5e81f0e66
Autor:
Lucia Gardini, Sarah M. Heissler, Claudia Arbore, Yi Yang, James R. Sellers, Francesco S. Pavone, Marco Capitanio
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-12 (2018)
Myosin-5B is an actin-based motor important for endosome recycling, but the molecular mechanism underlying its motility remains unknown. Here authors use single molecule imaging and high-speed laser tweezers to dissect the mechanoenzymatic properties
Externí odkaz:
https://doaj.org/article/a663d8e49b69440c972ca488748037c2