Zobrazeno 1 - 10
of 38
pro vyhledávání: '"James P. Hoeffler"'
Publikováno v:
BioTechniques, Vol 24, Iss 3, Pp 478-482 (1998)
Green fluorescent protein (GFP) and the Zeocin™-resistance gene Sh ble (ZeoR) were fused together to generate a bifunctional protein for the identification and selection of transfected mammalian cells. Expression of this hybrid selectable marker, G
Externí odkaz:
https://doaj.org/article/071ebf35373e4aeb95d794844899a17e
Autor:
Joseph M. Fernandez, James P. Hoeffler
Gene Expression Systems: Using Nature for the Art of Expression offers detailed information on a wide variety of gene expression systems from an array of organisms. It describes several different types of expression systems including transient, stabl
Autor:
Jeremiah R. Gilmore, Mario J. Patino, Ed J. Marsh, Jon J. Rowland, Kristen J. Hartman, Erin Gontang, Cathy L. Hernandez, Thomas J. Purcell, Heather M. Hull, Luis Foncerrada, Jeremiah Cornthwaite, James P. Hoeffler, Michelle Lynn Sindici, Robert Marcil, Rhiannon Hood, Kevin M. Mudd, John A. Heyman, Wai-Yee Lee
Publikováno v:
Genome Research. 9:383-392
The in vitro cloning of DNA molecules traditionally uses PCR amplification or site-specific restriction endonucleases to generate linear DNA inserts with defined termini and requires DNA ligase to covalently join those inserts to vectors with the cor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ed1c7715772156161872fc61f57eb90
https://doi.org/10.1101/gr.9.4.383
https://doi.org/10.1101/gr.9.4.383
Autor:
Donald F. Hunt, Rocco Falchetto, Lisa M. Dahm, Jill E. Hungerford, Chauncey W. Bowers, James P. Hoeffler, Jeffrey Shabanowitz, Charles D. Little
Publikováno v:
The Journal of Cell Biology
The assembly of the vessel wall from its cellular and extracellular matrix components is an essential event in embryogenesis. Recently, we used the descending aorta of the embryonic quail to define the morphological events that initiate the formation
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f5f00bc4cb6d3bdc09824db9a73607e
https://doi.org/10.1083/jcb.137.4.925
https://doi.org/10.1083/jcb.137.4.925
Autor:
S.M. Scanlon, Marijane Russell, K. Froning, R. Marcil, T. Griffiths, M. Almazan, B.D. Brown, James P. Hoeffler
Publikováno v:
BioTechniques. 22:982-987
The transfection efficiencies of a panel of eight uniquely different lipid reagents has been evaluated with two other commercially available lipids for use in transfecting a diversity of eukaryotic cell lines. The PerFect™ lipids are available indi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d583920ba1a95a45bc4ea4ec868e5137
https://doi.org/10.2144/97225pf02
https://doi.org/10.2144/97225pf02
Autor:
Nigel J. Ball, David A. Norris, James P. Hoeffler, Joseph G. Morelli, Joseph J. Yohn, Loren E. Golitz
Publikováno v:
Journal of Investigative Dermatology. 102(3):285-290
In this study we address whether there is an association between ras mutations and disease progression in malignant melanoma. DNA was extracted from 100 paraffin-embedded melanomas and sequences around the 12th, 13th and 61st codons of N-, H-, and K-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::000edd2fbdfe703f6c4b7d801bb6c15e
Autor:
David J. Kroll, Ruth Breckon, Heather T. Sponsel, Robert J. Anderson, James P. Hoeffler, Steven Jackson
Publikováno v:
Kidney International. 45:642-649
Escape from the antiproliferative effect of transforming growth factor-β 1 in LLC-PK 1 renal epithelial cells. Transforming growth factor-β 1 (TGF-β 1 ) usually inhibits proliferation of epithelial cells. We find that LLC-PK 1 renal tubular epithe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b2d6b2f641316ef97536a381a5d6935c
https://doi.org/10.1038/ki.1994.86
https://doi.org/10.1038/ki.1994.86
Publikováno v:
Molecular Endocrinology. 7:1256-1265
When antagonist-occupied steroid receptors have agonist-like effects, the clinical consequences are grave. We present evidence that human progesterone B-receptors (hPRB) when occupied by progesterone antagonists, inappropriately activate transcriptio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ee7e74fb39c02be10d4f292a4571d2f9
https://doi.org/10.1210/mend.7.10.8123133
https://doi.org/10.1210/mend.7.10.8123133
Autor:
Arthur Gutierrez-Hartmann, Pamela L. Mellon, Stephen M. Jackson, James P. Hoeffler, Kerry M. Barnhart
Publikováno v:
Molecular and Cellular Endocrinology. 96:167-176
The basic-helix-loop-helix (bHLH) class of transcriptional activators, important in the establishment of many different cell lineages, share two important properties: the ability to heterodimerize with other members of this family and to bind DNA con
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::20032d16873f468aa1a74c63b5b0c668
https://doi.org/10.1016/0303-7207(93)90107-u
https://doi.org/10.1016/0303-7207(93)90107-u
Autor:
James P. Hoeffler, Hany A. Abdel-Hafiz, David J. Kroll, Joyce W. Lustbader, Chang-You Chen, Arthur Gutierrez-Hartmann, Tere Marcell, Sean Simpson
Publikováno v:
DNA and Cell Biology. 12:441-453
A series of plasmid vectors, pRSET A, B, and C, have been developed for high-level protein expression in prokaryotes and have been characterized. Based upon the T7 RNA polymerase-driven pET system, the pRSET vectors encode recombinant proteins as fus
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a2c1d57b80ab2b1dcb4efd66aff3e094
https://doi.org/10.1089/dna.1993.12.441
https://doi.org/10.1089/dna.1993.12.441