Zobrazeno 1 - 10
of 16
pro vyhledávání: '"James McSwiggen"'
Autor:
Anya Vaish, James McSwiggen
Publikováno v:
Journal of Biosciences and Medicines. 10:84-97
Autor:
Narendra K. Vaish, Christopher Pasko, Karl Kossen, Vasant Jadhav, Hong Wang, Barry Polisky, Robert D. Jenison, Scott D Seiwert, James McSwiggen
Publikováno v:
Chemistry & Biology. 11:807-815
Many reports have suggested that target-activated ribozymes hold potential value as detection reagents. We show that a "half"-ribozyme ligase is activated similarly by three unstructured oligoribonucleotides representing the major sequence variants o
Autor:
Patricia Brown-Augsburger, Jennifer A. Lockridge, Dimple Kamboj, Kathleen M. Hillgren, Xin Min Yue, James McSwiggen
Publikováno v:
Journal of Pharmaceutical and Biomedical Analysis. 34:129-139
Ribozymes are RNA or modified RNA polymers capable of catalyzing cleavage reactions in target strands RNA, and are under development as human therapeutics. Previous methods used for quantitation of nucleic acid polymers in serum or plasma required ex
Autor:
James McSwiggen, Scott D. Seiwert, Barry Polisky, Narendra K. Vaish, Christopher Pasko, Vasant Jadhav, Karl Kossen, Lori E. Andrews
Publikováno v:
RNA. 9:1058-1072
We describe a strategy for the ultra-sensitive detection of nucleic acids using “half” ribozymes that are devoid of catalytic activity unless completed by a trans-acting target nucleic acid. The half-ribozyme concept was initially demonstrated us
Autor:
Melissa Arthur, Kurt Levy, Alexander Karpeisky, Laverna J. Alby, James McSwiggen, Jasenka Matulic-Adamic, Francine E. Wincott, Nassim Usman, Dan T. Stinchcomb, Thale C. Jarvis, John Gustofson, Anthony DiRenzo, Leonid Beigelman, Carolyn Gonzalez, Tod M. Woolf
Publikováno v:
Journal of Biological Chemistry. 271:29107-29112
Expression of the proto-oncogene c-myb is necessary for proliferation of vascular smooth muscle cells. We have developed synthetic hammerhead ribozymes that recognize and cleave c-myb RNA, thereby inhibiting cell proliferation. Herein, we describe a
Publikováno v:
Current Opinion in Structural Biology. 6:527-533
Of all the catalytic RNAs the hammerhead ribozyme is the most chemically modified and structurally studied. Such studies have resulted in improvements in the nuclease resistance of ribozymes, reductions in their size, and improvements in their cataly
Autor:
Alexander Karpeisky, Leonid Beigelman, Alex B. Burgin, James McSwiggen, Jasenka Matulic-Adamic, Carolyn Gonzales, Nassim Usman
Publikováno v:
Collection of Czechoslovak Chemical Communications. 61:271-275
Autor:
Leonid Beigelman, Alexander Karpeisky, James McSwiggen, Varykina G. Thackray, Kristi Jensen, Francine E. Wincott, Nassim Usman, Jasenka Matulic-Adamic, Kenneth G. Draper, Danuta Tracz, David Sweedler, Anil S. Modak, Carolyn Gonzalez, Susan Grimm, Peter Haeberli, Anthony DiRenzo
Publikováno v:
Journal of Biological Chemistry. 270:25702-25708
A systematic study of selectively modified, 36-mer hammerhead ribozymes has resulted in the identification of a generic, catalytically active and nuclease stable ribozyme motif containing 5 ribose residues, 29-30 2'-O-Me nucleotides, 1-2 other 2'-mod
Autor:
Robert H. Silverman, Ara G. Hovanessian, Ratan K. Maitra, Nigel A.J. McMillan, Shailesh Y. Desai, Bryan R.G. Williams, Ganes C. Sen, James McSwiggen
Publikováno v:
Virology. 204:823-827
The TAR sequence at the 5'-termini of all HIV-1 mRNA species forms a stable structure that is responsible for both transcriptional and translational regulation of HIV-1. Previously we and others reported that purified TAR RNA synthesized by in vitro
Publikováno v:
Journal of Biological Chemistry. 269:25856-25864
Ribozyme expression cassettes were constructed which generate trimmed, trans-acting ribozymes from longer transcripts through the action of a downstream cis-acting ribozyme. This self-processing system produces small, well-defined trans-acting ribozy