Expanding the Anti-Phl p 7 Antibody Toolkit: An Anti-Idiotype Nanobody Inhibitor

Autor: Susan K. Vester, Anna M. Davies, Rebecca L. Beavil, Balraj S. Sandhar, Andrew J. Beavil, Hannah J. Gould, Brian J. Sutton, James M. McDonnell

Publikováno v: Antibodies, Vol 12, Iss 4, p 75 (2023)

We have previously produced a toolkit of antibodies, comprising recombinant human antibodies of all but one of the human isotypes, directed against the polcalcin family antigen Phl p 7. In this work, we complete the toolkit of human antibody isotypes

Externí odkaz: https://doaj.org/article/d3b9137087384153b31f164c3220e2a6

IgE Trimers Drive SPE-7 Cytokinergic Activity

Autor: Heather J. Bax, Holly Bowen, Rebecca L. Beavil, Raymond Chung, Malcolm Ward, Anna M. Davies, Tihomir S. Dodev, James M. McDonnell, Andrew J. Beavil, Brian J. Sutton, Hannah J. Gould

Publikováno v: Scientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)

Abstract Degranulation of mast cells and basophils, with release of agents of the allergic response, ensues when multivalent antigens bind to and cross-link the cells’ receptor-bound IgE antibodies. A widely used commercial monoclonal IgE antibody,

Externí odkaz: https://doaj.org/article/43a4bcae3fcb47bd993a61cc883f32f7

NMR backbone assignment of the Cε4 domain of immunoglobulin E

Autor: Stefi V Benjamin, Paul I Creeke, Alistair James Henry, James M. McDonnell

Publikováno v: Biomolecular Nmr Assignments

Immunoglobulin E (IgE) plays a central role in allergic reactions. IgE is a dynamic molecule that is capable of undergoing large conformational changes. X-ray crystal structures of the Fc region of IgE in complex with various ligands have shown that

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::590d63b5b30042fa637fc2f7dd0c9177
http://europepmc.org/articles/PMC7069932

Generation and Characterization of Native and Sialic Acid-Deficient IgE

Autor: Alex J. McCraw, Richard A. Gardner, Anna M. Davies, Daniel I. R. Spencer, Melanie Grandits, Gerd K. Wagner, James M. McDonnell, Sophia N. Karagiannis, Alicia Chenoweth, Silvia Crescioli

Publikováno v: International Journal of Molecular Sciences; Volume 23; Issue 21; Pages: 13455
McCraw, A J, Gardner, R A, Davies, A M, Spencer, D I R, Grandits, M, Wagner, G K, McDonnell, J M, Karagiannis, S N, Chenoweth, A & Crescioli, S 2022, ' Generation and characterization of native and sialic acid-deficient IgE ', International Journal of Molecular Sciences, vol. 23, no. 21, 13455 . https://doi.org/10.3390/ijms232113455

Efficient characterization of IgE antibodies and their glycan structures is required for understanding their function in allergy and in the emerging AllergoOncology field for antibody immunotherapy. We report the generation, glyco-profiling and funct

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b451b7b5c13264efe61e3cc795e1b98d
https://doi.org/10.3390/ijms232113455

A Mass‐Spectrometry‐Based Modelling Workflow for Accurate Prediction of IgG Antibody Conformations in the Gas Phase

Autor: Kjetil Hansen, Andy M. Lau, Kevin Giles, James M. McDonnell, Weston B. Struwe, Brian J. Sutton, Argyris Politis

Publikováno v: Angewandte Chemie (International Ed. in English)

Immunoglobulins are biomolecules involved in defence against foreign substances. Flexibility is key to their functional properties in relation to antigen binding and receptor interactions. We have developed an integrative strategy combining ion mobil

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5fe3f54baa5c52fcefd7a0d26b91a57b
http://europepmc.org/articles/PMC6392142