DNA binding polarity, dimerization, and ATPase ring remodeling in the CMG helicase of the eukaryotic replisome

Autor: Alessandro Costa, Ludovic Renault, Paolo Swuec, Tatjana Petojevic, James J Pesavento, Ivar Ilves, Kirsty MacLellan-Gibson, Roland A Fleck, Michael R Botchan, James M Berger

Publikováno v: eLife, Vol 3 (2014)

The Cdc45/Mcm2-7/GINS (CMG) helicase separates DNA strands during replication in eukaryotes. How the CMG is assembled and engages DNA substrates remains unclear. Using electron microscopy, we have determined the structure of the CMG in the presence o

Externí odkaz: https://doaj.org/article/1c9a75ab3cf549beafe69c123e444555

An Improved Top-Down Mass Spectrometry Characterization of

Autor: Sarah R, Rommelfanger, Mowei, Zhou, Henna, Shaghasi, Shin-Cheng, Tzeng, Bradley S, Evans, Ljiljana, Paša-Tolić, James G, Umen, James J, Pesavento

Publikováno v: Journal of the American Society for Mass Spectrometry. 32(7)

We present an updated analysis of the linker and core histone proteins and their proteoforms in the green microalga

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::d38d9f8b9133f1dd2088aef32502d13d
https://pubmed.ncbi.nlm.nih.gov/34165968

How many human proteoforms are there?

Autor: Michael C. Jewett, Therese Wohlschlager, Vamsi K. Mootha, Jeremy Gunawardena, Steven M. Patrie, James J. Pesavento, Nicolas L. Young, Ole N. Jensen, Catherine Fenselau, Jeffrey N. Agar, Laura L. Kiessling, Sarah A. Slavoff, Evan R. Williams, Sharon J. Pitteri, Emma Lundberg, Lloyd M. Smith, Ruedi Aebersold, Alan Saghatelian, Salvatore Sechi, Marc Vidal, Nathan A. Yates, Tom W. Muir, Michael J. MacCoss, David R. Walt, Parag Mallick, Henry Rodriguez, Jennifer E. Van Eyk, Michael Snyder, Joseph A. Loo, Vicki H. Wysocki, Hartmut Schlüter, Bing Zhang, Milan Mrksich, Benjamin A. Garcia, Martin R. Larsen, Alexander R. Ivanov, Mark S. Baker, Ying Ge, Nevan J. Krogan, Catherine E. Costello, Paul J. Hergenrother, Neil L. Kelleher, I. Jonathan Amster, Rachel R. Ogorzalek Loo, Emily S. Boja, Mathias Uhlén, Benjamin F. Cravatt, Ronald C. Hendrickson, Wendy Sandoval, Paul M. Thomas, Christian G. Huber, Forest M. White, Carolyn R. Bertozzi

Publikováno v: Nature chemical biology, vol 14, iss 3
Aebersold, R, Agar, J N, Amster, I J, Baker, M S, Bertozzi, C R, Boja, E S, Costello, C E, Cravatt, B F, Fenselau, C, Garcia, B A, Ge, Y, Gunawardena, J, Hendrickson, R C, Hergenrother, P J, Huber, C G, Ivanov, A R, Jensen, O N, Jewett, M C, Kelleher, N L, Kiessling, L L, Krogan, N J, Larsen, M R, Loo, J A, Ogorzalek Loo, R R, Lundberg, E, MacCoss, M J, Mallick, P, Mootha, V K, Mrksich, M, Muir, T W, Patrie, S M, Pesavento, J J, Pitteri, S J, Rodriguez, H, Saghatelian, A, Sandoval, W, Schlüter, H, Sechi, S, Slavoff, S A, Smith, L M, Snyder, M P, Thomas, P M, Uhlén, M, Van Eyk, J E, Vidal, M, Walt, D R, White, F M, Williams, E R, Wohlschlager, T, Wysocki, V H, Yates, N A, Young, N L & Zhang, B 2018, ' How many human proteoforms are there? ', Nature Chemical Biology, vol. 14, no. 3, pp. 206-214 . https://doi.org/10.1038/nchembio.2576
PMC

Despite decades of accumulated knowledge about proteins and their post-translational modifications (PTMs), numerous questions remain regarding their molecular composition and biological function. One of the most fundamental queries is the extent to w

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::112005919432b51c4f87047a1ed9bdfa
https://escholarship.org/uc/item/1r85h1h0

Activation of the MCM2-7 Helicase by Association with Cdc45 and GINS Proteins

Autor: Tatjana Petojevic, James J. Pesavento, Michael R. Botchan, Ivar Ilves

Publikováno v: Molecular Cell. 37:247-258

MCM2-7 proteins provide essential helicase functions in eukaryotes at chromosomal DNA replication forks. During the G1 phase of the cell cycle, they remain loaded on DNA but are inactive. We have used recombinant methods to show that the Drosophila M

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae37cb321bfe6041276e267003cdb08e
https://doi.org/10.1016/j.molcel.2009.12.030

Interpreting Top-Down Mass Spectra Using Spectral Alignment

Autor: James J. Pesavento, Neil L. Kelleher, Pavel A. Pevzner, Ari Frank, Craig A. Mizzen

Publikováno v: Analytical Chemistry. 80:2499-2505

Recent advances in mass spectrometry instrumentation, such as FTICR and OrbiTrap, have made it possible to generate high-resolution spectra of entire proteins. While these methods offer new opportunities for performing "top-down" studies of proteins,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cf8304991b59e71e0123f436478a9da2
https://doi.org/10.1021/ac702324u