Zobrazeno 1 - 10
of 33
pro vyhledávání: '"James J, Pesavento"'
Autor:
James J. Pesavento, James G. Umen, Henna Shaghasi, Mowei Zhou, Bradley S. Evans, Sarah R Rommelfanger, Shin-Cheng Tzeng, Ljiljana Paša-Tolić
Publikováno v:
Journal of the American Society for Mass Spectrometry. 32:1671-1688
We present an updated analysis of the linker and core histone proteins and their proteoforms in the green microalga Chlamydomonas reinhardtii by top-down mass spectrometry (TDMS). The combination of high-resolution liquid chromatographic separation,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::968fc0f1d586ceed99cc7b07072e37d6
https://doi.org/10.1021/jasms.1c00029
https://doi.org/10.1021/jasms.1c00029
Autor:
Alessandro Costa, Ludovic Renault, Paolo Swuec, Tatjana Petojevic, James J Pesavento, Ivar Ilves, Kirsty MacLellan-Gibson, Roland A Fleck, Michael R Botchan, James M Berger
Publikováno v:
eLife, Vol 3 (2014)
The Cdc45/Mcm2-7/GINS (CMG) helicase separates DNA strands during replication in eukaryotes. How the CMG is assembled and engages DNA substrates remains unclear. Using electron microscopy, we have determined the structure of the CMG in the presence o
Externí odkaz:
https://doaj.org/article/1c9a75ab3cf549beafe69c123e444555
Autor:
Sarah R, Rommelfanger, Mowei, Zhou, Henna, Shaghasi, Shin-Cheng, Tzeng, Bradley S, Evans, Ljiljana, Paša-Tolić, James G, Umen, James J, Pesavento
Publikováno v:
Journal of the American Society for Mass Spectrometry. 32(7)
We present an updated analysis of the linker and core histone proteins and their proteoforms in the green microalga
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::d38d9f8b9133f1dd2088aef32502d13d
https://pubmed.ncbi.nlm.nih.gov/34165968
https://pubmed.ncbi.nlm.nih.gov/34165968
Publikováno v:
Methods (San Diego, Calif.). 184
The development of universal, broadly applicable methods for histone extraction from animal cells and tissues has unlocked the ability to compare these epigenetic-influencing proteins across tissue types, healthy and diseased states, and cancerous ve
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7f3141b3b88feda4634e17c1bbc7bb22
https://pubmed.ncbi.nlm.nih.gov/32014607
https://pubmed.ncbi.nlm.nih.gov/32014607
Autor:
Michael C. Jewett, Therese Wohlschlager, Vamsi K. Mootha, Jeremy Gunawardena, Steven M. Patrie, James J. Pesavento, Nicolas L. Young, Ole N. Jensen, Catherine Fenselau, Jeffrey N. Agar, Laura L. Kiessling, Sarah A. Slavoff, Evan R. Williams, Sharon J. Pitteri, Emma Lundberg, Lloyd M. Smith, Ruedi Aebersold, Alan Saghatelian, Salvatore Sechi, Marc Vidal, Nathan A. Yates, Tom W. Muir, Michael J. MacCoss, David R. Walt, Parag Mallick, Henry Rodriguez, Jennifer E. Van Eyk, Michael Snyder, Joseph A. Loo, Vicki H. Wysocki, Hartmut Schlüter, Bing Zhang, Milan Mrksich, Benjamin A. Garcia, Martin R. Larsen, Alexander R. Ivanov, Mark S. Baker, Ying Ge, Nevan J. Krogan, Catherine E. Costello, Paul J. Hergenrother, Neil L. Kelleher, I. Jonathan Amster, Rachel R. Ogorzalek Loo, Emily S. Boja, Mathias Uhlén, Benjamin F. Cravatt, Ronald C. Hendrickson, Wendy Sandoval, Paul M. Thomas, Christian G. Huber, Forest M. White, Carolyn R. Bertozzi
Publikováno v:
Nature chemical biology, vol 14, iss 3
Aebersold, R, Agar, J N, Amster, I J, Baker, M S, Bertozzi, C R, Boja, E S, Costello, C E, Cravatt, B F, Fenselau, C, Garcia, B A, Ge, Y, Gunawardena, J, Hendrickson, R C, Hergenrother, P J, Huber, C G, Ivanov, A R, Jensen, O N, Jewett, M C, Kelleher, N L, Kiessling, L L, Krogan, N J, Larsen, M R, Loo, J A, Ogorzalek Loo, R R, Lundberg, E, MacCoss, M J, Mallick, P, Mootha, V K, Mrksich, M, Muir, T W, Patrie, S M, Pesavento, J J, Pitteri, S J, Rodriguez, H, Saghatelian, A, Sandoval, W, Schlüter, H, Sechi, S, Slavoff, S A, Smith, L M, Snyder, M P, Thomas, P M, Uhlén, M, Van Eyk, J E, Vidal, M, Walt, D R, White, F M, Williams, E R, Wohlschlager, T, Wysocki, V H, Yates, N A, Young, N L & Zhang, B 2018, ' How many human proteoforms are there? ', Nature Chemical Biology, vol. 14, no. 3, pp. 206-214 . https://doi.org/10.1038/nchembio.2576
PMC
Aebersold, R, Agar, J N, Amster, I J, Baker, M S, Bertozzi, C R, Boja, E S, Costello, C E, Cravatt, B F, Fenselau, C, Garcia, B A, Ge, Y, Gunawardena, J, Hendrickson, R C, Hergenrother, P J, Huber, C G, Ivanov, A R, Jensen, O N, Jewett, M C, Kelleher, N L, Kiessling, L L, Krogan, N J, Larsen, M R, Loo, J A, Ogorzalek Loo, R R, Lundberg, E, MacCoss, M J, Mallick, P, Mootha, V K, Mrksich, M, Muir, T W, Patrie, S M, Pesavento, J J, Pitteri, S J, Rodriguez, H, Saghatelian, A, Sandoval, W, Schlüter, H, Sechi, S, Slavoff, S A, Smith, L M, Snyder, M P, Thomas, P M, Uhlén, M, Van Eyk, J E, Vidal, M, Walt, D R, White, F M, Williams, E R, Wohlschlager, T, Wysocki, V H, Yates, N A, Young, N L & Zhang, B 2018, ' How many human proteoforms are there? ', Nature Chemical Biology, vol. 14, no. 3, pp. 206-214 . https://doi.org/10.1038/nchembio.2576
PMC
Despite decades of accumulated knowledge about proteins and their post-translational modifications (PTMs), numerous questions remain regarding their molecular composition and biological function. One of the most fundamental queries is the extent to w
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::112005919432b51c4f87047a1ed9bdfa
https://escholarship.org/uc/item/1r85h1h0
https://escholarship.org/uc/item/1r85h1h0
Publikováno v:
Journal of proteome research. 17(1)
The unicellular microalga Chlamydomonas reinhardtii has played an instrumental role in the development of many new fields (bioproducts, biofuels, etc.) as well as the advancement of basic science (photosynthetic apparati, flagellar function, etc.). C
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d57bcde770d0571b4c07e0aa5478055
https://pubmed.ncbi.nlm.nih.gov/29198113
https://pubmed.ncbi.nlm.nih.gov/29198113
Publikováno v:
Molecular Cell. 37:247-258
MCM2-7 proteins provide essential helicase functions in eukaryotes at chromosomal DNA replication forks. During the G1 phase of the cell cycle, they remain loaded on DNA but are inactive. We have used recombinant methods to show that the Drosophila M
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae37cb321bfe6041276e267003cdb08e
https://doi.org/10.1016/j.molcel.2009.12.030
https://doi.org/10.1016/j.molcel.2009.12.030
Publikováno v:
Journal of Biological Chemistry. 283:14927-14937
Quantitative proteomics has focused heavily on correlating protein abundances, ratios, and dynamics by developing methods that are protein expression-centric (e.g. isotope coded affinity tag, isobaric tag for relative and absolute quantification, etc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3e8a2599d9ad3fed6acd8daa214275dc
https://doi.org/10.1074/jbc.m709796200
https://doi.org/10.1074/jbc.m709796200
Publikováno v:
Analytical Chemistry. 80:2499-2505
Recent advances in mass spectrometry instrumentation, such as FTICR and OrbiTrap, have made it possible to generate high-resolution spectra of entire proteins. While these methods offer new opportunities for performing "top-down" studies of proteins,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cf8304991b59e71e0123f436478a9da2
https://doi.org/10.1021/ac702324u
https://doi.org/10.1021/ac702324u
Publikováno v:
Molecular & Cellular Proteomics. 6:1510-1526
Recent developments in top down mass spectrometry have enabled closely related histone variants and their modified forms to be identified and quantitated with unprecedented precision, facilitating efforts to better understand how histones contribute
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e2799da8fb7823d073333358b825b903
https://doi.org/10.1074/mcp.m600404-mcp200
https://doi.org/10.1074/mcp.m600404-mcp200