Zobrazeno 1 - 10
of 14
pro vyhledávání: '"James H. Kurasawa"'
Autor:
James H. Kurasawa, Andrew Park, Carrie R. Sowers, Rebecca A. Halpin, Andrey Tovchigrechko, Claire L. Dobson, Albert E. Schmelzer, Changshou Gao, Susan D. Wilson, Yasuhiro Ikeda
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 19, Iss , Pp 330-340 (2020)
The recombinant adeno-associated virus (AAV) vector is one of the most utilized viral vectors in gene therapy due to its robust, long-term in vivo transgene expression and low toxicity. One major hurdle for clinical AAV applications is large-scale ma
Externí odkaz:
https://doaj.org/article/18e78126ac4a4698ab36a2d608981c8b
Autor:
Haarin Chun, James H. Kurasawa, Philip Olivares, Ekaterina S. Marakasova, Svetlana A. Shestopal, Gabriela U. Hassink, Elena Karnaukhova, Mary Migliorini, Juliet O. Obi, Ally K. Smith, Patrick L. Wintrode, Prasannavenkatesh Durai, Keunwan Park, Daniel Deredge, Dudley K. Strickland, Andrey G. Sarafanov
Publikováno v:
Journal of Thrombosis and Haemostasis. 20:2255-2269
Deficiency in blood coagulation factor VIII (FVIII) results in life-threating bleeding (hemophilia A) treated by infusions of FVIII concentrates. To improve disease treatment, FVIII has been modified to increase its plasma half-life, which requires u
Autor:
Andrey Tovchigrechko, Yasuhiro Ikeda, Claire Dobson, Andrew Park, Susan Wilson, Rebecca A. Halpin, Changshou Gao, Carrie R. Sowers, Albert E. Schmelzer, James H. Kurasawa
Publikováno v:
Molecular Therapy. Methods & Clinical Development
Molecular Therapy: Methods & Clinical Development, Vol 19, Iss, Pp 330-340 (2020)
Molecular Therapy: Methods & Clinical Development, Vol 19, Iss, Pp 330-340 (2020)
The recombinant adeno-associated virus (AAV) vector is one of the most utilized viral vectors in gene therapy due to its robust, long-term in vivo transgene expression and low toxicity. One major hurdle for clinical AAV applications is large-scale ma
Autor:
Svetlana A Shestopal, Andrey G Sarafanov, Ekaterina S. Marakasova, James H. Kurasawa, Haarin Chun, Elena Karnaukhova, Dudley K. Strickland, Nancy E. Hernandez, Philip Olivares, Gabriela U. Hassink
Publikováno v:
The Journal of Biological Chemistry
The low-density lipoprotein receptor (LDLR) family of receptors are cell-surface receptors that internalize numerous ligands and play crucial role in various processes, such as lipoprotein metabolism, hemostasis, fetal development, etc. Previously, r
Autor:
Jian-Jiang Hao, John H. McVey, Yideng Liang, James H. Kurasawa, Mikhail V Ovanesov, Elena Karnaukhova, Timothy K Lee, M. Lin, Andrey G Sarafanov, Svetlana A Shestopal
Publikováno v:
Journal of Thrombosis and Haemostasis. 15:709-720
Essentials Recombinant factor VIII (FVIII) is known to be expressed at a low level in cell culture. To increase expression, we used codon-optimization of a B-domain deleted FVIII (BDD-FVIII). This resulted in 7-fold increase of the expression level i
Autor:
Yelena Lazdun, Eric Tu, Susan Wilson, Kelly McGlinchey, Katie Streicher, Leslie Wetzel, Zachary A. Cooper, James H. Kurasawa, Karen Coffman
Publikováno v:
Cancer Research. 80:911-911
It has been reported that NSCLC patients with EGFR mutation do not respond to anti-PD-1(L1) therapies as well as patients without the mutation. We have previously shown that EGFR mutant tumors express more CD73 compared to EGFR wildtype tumors and we
Mapping the Binding Region on the Low Density Lipoprotein Receptor for Blood Coagulation Factor VIII
Autor:
James H. Kurasawa, Svetlana A Shestopal, Andrey G Sarafanov, Elena Karnaukhova, Timothy K Lee, Evi Struble
Publikováno v:
Journal of Biological Chemistry. 288:22033-22041
Low density lipoprotein receptor (LDLR) was shown to mediate clearance of blood coagulation factor VIII (FVIII) from the circulation. To elucidate the mechanism of interaction of LDLR and FVIII, our objective was to identify the region of the recepto
Autor:
Andrey G Sarafanov, James H. Kurasawa, Svetlana A Shestopal, Timothy K Lee, Naveen Jha, Mikhail V Ovanesov
Publikováno v:
Protein Expression and Purification. 88:201-206
A recombinant single-chain variable antibody fragment (scFv) KM33 was previously described as a ligand that can inhibit the function of blood coagulation factor VIII (FVIII). This scFv was previously derived from an individual with anti-FVIII antibod
Autor:
Hervé Chambost, James H. Kurasawa, Irina A. Demina, Andrey G Sarafanov, Mikhail A. Panteleev, Fazly I. Ataullakhanov, Nadezhda A. Podoplelova, Marie-Christine Alessi, Anastasia N. Sveshnikova, Sergey A. Vasil’ev
Publikováno v:
BBA-Biochimica et Biophysica Acta
BBA-Biochimica et Biophysica Acta, Elsevier, 2016, 1858 (6), pp.1216-27
BBA-Biochimica et Biophysica Acta, Elsevier, 2016, 1858 (6), pp.1216-27
International audience; Binding of coagulation factors X (fX) and Xa (fXa) to activated platelets is required for the formation of membrane-dependent enzymatic complexes of intrinsic tenase and prothrombinase. We carried out an in-depth characterizat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c50c2cf8d1790d127ed0ea5e67cb773d
https://hal.archives-ouvertes.fr/hal-01478132
https://hal.archives-ouvertes.fr/hal-01478132
Autor:
Samuel A. Woodle, Svetlana A Shestopal, Andrey G Sarafanov, Mikhail V Ovanesov, Timothy K Lee, James H. Kurasawa
Publikováno v:
Biochemistry. 54(2)
Low-density lipoprotein receptor-related protein 1 (LRP) mediates clearance of blood coagulation factor VIII (FVIII). In LRP, FVIII binds the complement-type repeats (CRs) of clusters II and IV, which also bind a majority of other LRP ligands. No lig