Zobrazeno 1 - 10
of 21
pro vyhledávání: '"James H. Flanagan"'
Publikováno v:
ELECTROPHORESIS. 24:3147-3153
The detection of point and other simple mutations in DNA is important for cancer research and diagnosis and other biological studies. Capillary electrophoresis has been successfully used for separating DNA fragments. However, a low-viscosity polymer
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::79338581e4dfc77a196d9f1df2ae195d
https://doi.org/10.1002/elps.200305572
https://doi.org/10.1002/elps.200305572
Autor:
Emanuel Waddell, James H. Flanagan, Shaheer H. Kahn, Sarah E. Romero, Clyde V. Owens, Robert P. Hammer, Steven A. Soper
Publikováno v:
Analytical Chemistry. 70:2676-2684
A series of near-IR fluorescent dyes were prepared which contained an intramolecular heavy atom for altering the fluorescence lifetimes to produce a set of probes appropriate for base-calling in a single-lane DNA sequencing format. The heavy-atom mod
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9812c412c266928788f679e2764943e8
https://doi.org/10.1021/ac980018g
https://doi.org/10.1021/ac980018g
Publikováno v:
Bioconjugate Chemistry. 8:751-756
The syntheses of three novel functionalized tricarbocyanine dyes are described. These dyes containing isothiocyanate and succinimidyl ester functional groups are reactive toward primary amines and can be used as fluorescent probes for biologically pe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b29682acca421f6d347ef33d0864a99
https://doi.org/10.1021/bc970113g
https://doi.org/10.1021/bc970113g
Autor:
James H. Flanagan, Benjamin L. Legendre, Steven A. Soper, Robert P. Hammer, Daryl C. Williams
Publikováno v:
IEEE Journal of Selected Topics in Quantum Electronics. 2:1129-1139
Laser-induced fluorescence detection has become the detection strategy of choice in many large-scale DNA sequencing applications due to its ease of Implementation, sensitivity and the ability to identify the constituent bases of DNA in a single separ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e910b2585db6924f9da36a214b39d3cd
https://doi.org/10.1109/2944.577345
https://doi.org/10.1109/2944.577345
Publikováno v:
Analytical Chemistry. 67:341-347
We have investigated the effects of mixed organic/aqueous carrier buffers on the chromatographic efficiency and detectabilipy of native near-infrared (near-IR) dyes and near-IR dye-labeled amino acids separated using capillary electrophoresis (CE). T
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::91c16b01db77f098b9200d0d4808ad61
https://doi.org/10.1021/ac00098a018
https://doi.org/10.1021/ac00098a018
Publikováno v:
Journal of capillary electrophoresis and microchip technology. 10(1-2)
Heteroduplex analysis is the most popular method for double-stranded DNA mutation detection thus far. Since different DNA fragments have various melting temperatures due to different base pair compositions and sizes, the PCR-amplified DNA fragments n
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::918e2d875a2a99e0ee0c0aa130b14d10
https://pubmed.ncbi.nlm.nih.gov/17685240
https://pubmed.ncbi.nlm.nih.gov/17685240
Autor:
Suzzane J, Lassiter, Wieslaw, Stryjewski, Clyde V, Owens, James H, Flanagan, Robert P, Hammer, Shaheer, Khan, Steven A, Soper
Publikováno v:
Electrophoresis. 23(10)
We have investigated the sample preparation and electrophoresis conditions necessary to prepare DNA sequencing samples appropriate for use with near-infrared (IR) fluorescent labels with dye identification accomplished via lifetime techniques. It was
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::de3c96d3ff2a2928badde35a7d716828
https://pubmed.ncbi.nlm.nih.gov/12116159
https://pubmed.ncbi.nlm.nih.gov/12116159
Publikováno v:
SPIE Proceedings.
A series of near-IR fluorescent dyes have been prepared which contain an intramolecular heavy atom for altering the photophysics to produce a set of probes appropriate for single lane DNA sequencing applications. The identification of the terminal nu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::64ebed91de632fa288350cae1de63166
https://doi.org/10.1117/12.273529
https://doi.org/10.1117/12.273529
Autor:
Daryl C. Williams, Benjamin L. Legendre, Clyde V. Owens, Robert P. Hammer, James H. Flanagan, Yolanda Y. Davidson, Steven A. Soper
Publikováno v:
SPIE Proceedings.
Our group is developing optical solid-state, near-IR (NIR) fluorescence detection systems for the analyses ofDNA in restrictionmapping and sequencing applications. Specifically, we are investigating a base-calling scheme using fluorescence lifetimedi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f05554f44aff97c1cf0014a978ad3c87
https://doi.org/10.1117/12.237611
https://doi.org/10.1117/12.237611