Zobrazeno 1 - 10
of 12
pro vyhledávání: '"James G. Nadeau"'
Publikováno v:
Journal of Biomedical Materials Research. 40:92-103
A biophysical model linking fibrin polymerization kinetics (following release from a thrombin-fibrinogen complex), coagulation time, and competitive inhibition of thrombin illustrates the utility of thrombin-binding ligands as anticoagulants in blood
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::70354fba06622038e75eea0c7bcf2b66
https://doi.org/10.1002/(sici)1097-4636(199804)40:1<92::aid-jbm11>3.0.co
https://doi.org/10.1002/(sici)1097-4636(199804)40:1<92::aid-jbm11>3.0.co
Publikováno v:
Clinical Chemistry. 42:9-13
Strand displacement amplification (SDA) is an isothermal, in vitro method of amplifying a DNA sequence for diagnostic purposes. We have combined SDA with fluorescence polarization detection in a closed, homogeneous format. A fluorescently labeled oli
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::86b66ec8b58e8c6ff67e42cf275c2237
https://doi.org/10.1093/clinchem/42.1.9
https://doi.org/10.1093/clinchem/42.1.9
Publikováno v:
Molecular and Cellular Probes. 9:399-403
Strand displacement amplification (SDA) is an isothermal, in vitro method of amplifying a target DNA sequence. We performed SDA in the presence of a 5'-32P-oligodeoxynucleotide detector probe that contains a target binding sequence at its 3'-end and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2875d9e22b48f0e854d16b2fde7c4508
https://doi.org/10.1006/mcpr.1995.0062
https://doi.org/10.1006/mcpr.1995.0062
Autor:
James G. Nadeau, G. Terrance Walker, Colleen M Nycz, Daryl D. Shank, James L. Schram, Patricia A. Spears
Publikováno v:
Nucleic Acids Research. 22:2670-2677
Strand Displacement Amplification (SDA) is an isothermal, in vitro method of amplifying a DNA target sequence prior to detection [Walker et al (1992) Nucleic Acids Res., 20, 1691-1693]. Here we describe a multiplex form of SDA that allows two target
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29de39398f12116877397ff0b6684ccf
https://doi.org/10.1093/nar/22.13.2670
https://doi.org/10.1093/nar/22.13.2670
Publikováno v:
Nucleic Acids Research. 20:1691-1696
Strand Displacement Amplification (SDA) is an isothermal, in vitro nucleic acid amplification technique based upon the ability of HincII to nick the unmodified strand of a hemiphosphorothioate form of its recognition site, and the ability of exonucle
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::caf877358b0ce271ccb424e408ace752
https://doi.org/10.1093/nar/20.7.1691
https://doi.org/10.1093/nar/20.7.1691
Publikováno v:
Clinical chemistry. 49(10)
Background: The BD ProbeTec™ ET System is based on isothermal strand displacement amplification (SDA) of target nucleic acid coupled with homogeneous real-time detection using fluorescent probes. We have developed a novel, rapid method using this p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a402b30d4e4e2797a273e122618a108b
https://pubmed.ncbi.nlm.nih.gov/14500584
https://pubmed.ncbi.nlm.nih.gov/14500584
Publikováno v:
Analytical biochemistry. 276(2)
Strand displacement amplification (SDA) is an isothermal nucleic acid amplification method based on the primer-directed nicking activity of a restriction enzyme and the strand displacement activity of an exonuclease-deficient polymerase. Here we desc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b83380c166aa0c95083cc3b61a71afbb
https://pubmed.ncbi.nlm.nih.gov/10603241
https://pubmed.ncbi.nlm.nih.gov/10603241
Strand displacement amplification (9SDA) is an isothermal in vitro method of amplifying a DNA sequence prior to its detection. We have combined SDA with fluorescence polarization detection. A 5'-fluorescein-labelled oligodeoxynucleotide detector prob
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b6407773fb8a550ee53e839e5374335c
https://europepmc.org/articles/PMC145624/
https://europepmc.org/articles/PMC145624/
Autor:
J.A. Down, W.E. Keating, C.A. Spargo, M.S. Dey, A.H. Walters, D.R. Howard, Michael C. Little, P. Zwadyk, Colleen M Nycz, James G. Nadeau, V.R. Neece, G T Walker, Stewart Russel Jurgensen
Publikováno v:
Molecular Methods for Virus Detection
This chapter introduces a DNA probe test that is based on strand displacement amplification (SDA) and chemiluminescence detection. A step-by-step protocol is presented for amplification and detection of a DNA sequence specific to Myobacterium tubercu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e9c7b546763f494dd48e5f75a3682180
https://doi.org/10.1016/b978-012748920-9/50016-9
https://doi.org/10.1016/b978-012748920-9/50016-9
Autor:
Douglas P. Malinowski, Michael C. Little, James G. Nadeau, Rüdiger Rüger, Amy Alexander, Melinda S. Fraiser, David Segev, Euan K. James, Erko Stackebrandt, Werner Liesack, G. Terrance Walker, Keith C. Backman, George H. ShimerJr., James L. Schram
Publikováno v:
Nonradioactive Labeling and Detection of Biomolecules ISBN: 9783662001462
The polymerase chain reaction (PCR) represents the most common and widespread method for the direct amplification of specific sequences of nucleic acid target molecules. The basic reaction is comprised of three steps: 1. Denaturation of the target DN
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::731183cfee5f3c434fd125ab479d91af
https://doi.org/10.1007/978-3-662-00144-8_16
https://doi.org/10.1007/978-3-662-00144-8_16