Zobrazeno 1 - 6
of 6
pro vyhledávání: '"James Edward Christner"'
Publikováno v:
Analytical Biochemistry. 72:283-292
A unique rapid method for assaying specific binding reactions with ligand-cofactor conjugates and a bioluminescent reaction is described. Biotin and 2,4-dinitrofluorobenzene were coupled covalently to the free amino residue of nicotinamide 6-(2-amino
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4efe7f52ef0b9f0a45b2d0da7ee616a4
https://doi.org/10.1016/0003-2697(76)90531-5
https://doi.org/10.1016/0003-2697(76)90531-5
Autor:
Robert C. Boguslaski, Robert J. Carrico, James Edward Christner, Robert T. Buckler, Hartmut R. Schroeder, Kwok-Kam Yeung
Publikováno v:
Analytical Biochemistry. 76:95-110
A new method was developed to monitor specific protein binding reactions with an ATP-labeled ligand and firefly luciferase. The ligand, 2,4-dinitrobenzene, was covalently coupled to four ATP derivatives and three of these conjugates were measured qua
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f73e0194661530132a5cca23d1ecea43
https://doi.org/10.1016/0003-2697(76)90267-0
https://doi.org/10.1016/0003-2697(76)90267-0
Publikováno v:
Analytical Biochemistry. 72:271-282
Specific binding reactions were monitored by a new enzymatic method which employs cofactor labeled ligands. The ligands biotin and 2,4-dinitrofluorobenzene were coupled covalently to a derivative of NAD, nicotinamide 6-(2-aminoethylamino) purine dinu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b1e91c4a07ecacb1d9911102166c325d
https://doi.org/10.1016/0003-2697(76)90530-3
https://doi.org/10.1016/0003-2697(76)90530-3
Publikováno v:
Steroids. 24(3)
A rapid, precise, and accurate radioimmunoassay for unconjugated estriol in pregnancy plasma has been developed which makes use of the adsorptive properties of Sephadex. The estriol is extracted from plasma by adsorption onto a small column of Sephad
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c660d4871c24dbd183b70c135b43f30d
https://pubmed.ncbi.nlm.nih.gov/4471979
https://pubmed.ncbi.nlm.nih.gov/4471979
Publikováno v:
Enzyme Engineering ISBN: 9781468469875
Various methods have been devised for monitoring specific protein-binding assays with enzymic reactions (1–4). In one method the ligand is coupled covalently to NAD; and this conjugate is measured at low levels by means of enzymic cycling reactions
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fc215cafad236de4d41fb317cea7600a
https://doi.org/10.1007/978-1-4684-6985-1_87
https://doi.org/10.1007/978-1-4684-6985-1_87
Autor:
R.D. Johnson, Robert C. Boguslaski, Marion C. Fetter, Robert J. Carrico, Robert T. Buckler, John F. Burd, James Edward Christner
Publikováno v:
Analytical biochemistry. 77(1)
Specific protein-binding reactions were monitored utilizing ligand-fluorescent dye conjugates which were substrates for porcine esterase. Biotin was coupled directly to umbelliferone through an ester bond, while 2,4-dinitrophenyl derivatives were con
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c556d55d7978af6ecbd8960fbd524fef
https://pubmed.ncbi.nlm.nih.gov/12682
https://pubmed.ncbi.nlm.nih.gov/12682