Development of a replication-competent lentivirus assay for dendritic cell-targeting lentiviral vectors

Autor: Daniel C Farley, Laura McCloskey, Barbara A Thorne, Semih U Tareen, Christopher J Nicolai, David J Campbell, Richard Bannister, Hannah J Stewart, Laura JE Pearson, Bentley J Moyer, Scott H Robbins, Leah Zielinski, Tae Kim, Pippa A Radcliffe, Kyriacos A Mitrophanous, Wayne R Gombotz, James E Miskin, Brenna Kelley-Clarke

Publikováno v: Molecular Therapy: Methods & Clinical Development, Vol 2, Iss , Pp - (2015)

It is a current regulatory requirement to demonstrate absence of detectable replication-competent lentivirus (RCL) in lentiviral vector products prior to use in clinical trials. Immune Design previously described an HIV-1-based integration-deficient

Externí odkaz: https://doaj.org/article/7f7c5943dd9b4748b580d44545f0e674

Nuclear and nucleolar localization of an African swine fever virus protein, I14L, that is similar to the herpes simplex virus-encoded virulence factor ICP34.5

Autor: Lynnette C. Goatley, Geoffrey L. Smith, Jef M. Hammond, James E. Miskin, Marie B. Marron, Susan C. Jacobs, Charles C. Abrams, Linda K. Dixon

Publikováno v: Journal of General Virology. 80:525-535

PCR analysis of the genomes of 18 different African swine fever virus (ASFV) isolates showed that the I14L open reading frame (ORF) was present as either a long form or short form in all of the isolates. Sequencing of the ORF from eight isolates conf

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b684c742397288235ebd60f7e92e76c2
https://doi.org/10.1099/0022-1317-80-3-525

The CD2v protein of African swine fever virus interacts with the actin-binding adaptor protein SH3P7

Autor: P. C. Kay-Jackson, James E. Miskin, J. Wienands, R. M. E. Parkhouse, L. Cox, Lynnette C. Goatley, Linda K. Dixon

Publikováno v: The Journal of general virology. 85(Pt 1)

The predicted extracellular domain of the CD2v protein of African swine fever virus (ASFV) shares significant similarity to that of the CD2 protein in T cells but has a unique cytoplasmic domain of unknown function. Here we have shown that CD2v is ex

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a79c89f6d3c88106bb0ee159ad4b1503
https://pubmed.ncbi.nlm.nih.gov/14718626

African swine fever virus protein p54 interacts with the microtubular motor complex through direct binding to light-chain dynein

Autor: Ignacio Rodríguez-Crespo, Covadonga Alonso, Carmen Cantó, James E. Miskin, Patricia Fernandez-Zapatero, Linda K. Dixon, José M. Escribano, Bruno Hernáez, Lourdes Soto

Publikováno v: Repositorio de Resultados de Investigación del INIA
INIA: Repositorio de Resultados de Investigación del INIA
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA

Dynein is a minus-end-directed microtubule-associated motor protein involved in cargo transport in the cytoplasm. African swine fever virus (ASFV), a large DNA virus, hijacks the microtubule motor complex cellular transport machinery during virus inf

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f96bd028c1af7fea9f7dd4e0a686645e
https://hdl.handle.net/20.500.12792/5870

An ARID family protein binds to the African swine fever virus encoded ubiquitin conjugating enzyme, UBCv1

Autor: James E. Miskin, Linda K. Dixon, Wallace D. Bulimo

Publikováno v: FEBS Letters. (1):17-22

The NH2-terminal end of a protein, named SMCp, which contains an ARID (A/T rich interaction domain) DNA binding domain and is similar to the mammalian SMCY/SMCX proteins and retinoblastoma binding protein 2, was shown to bind the African swine fever

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::976888d3e43326ea7ccc7f154f5c4330