Zobrazeno 1 - 10
of 384
pro vyhledávání: '"James E Haber"'
Autor:
Elena Sapède, Neal Sugawara, Randall G Tyers, Yuko Nakajima, Mosammat Faria Afreen, Jesselin Romero Escobar, James E Haber
Publikováno v:
PLoS Genetics, Vol 20, Iss 2, p e1010527 (2024)
Single-strand annealing (SSA) is initiated when a double strand break (DSB) occurs between two flanking repeated sequences, resulting in a deletion that leaves a single copy of the repeat. We studied budding yeast strains carrying two 200-bp URA3 seq
Externí odkaz:
https://doaj.org/article/2f6e0f4f03a74c2cbbfbdd37dd653cfc
Autor:
Jihyun Choi, Muwen Kong, Danielle N Gallagher, Kevin Li, Gabriel Bronk, Yiting Cao, Eric C Greene, James E Haber
Publikováno v:
PLoS Genetics, Vol 18, Iss 9, p e1010056 (2022)
Using budding yeast, we have studied Rad51-dependent break-induced replication (BIR), where the invading 3' end of a site-specific double-strand break (DSB) and a donor template share 108 bp of homology that can be easily altered. BIR still occurs ab
Externí odkaz:
https://doaj.org/article/9b7086fc651b42c1a94cc5e31d1434a4
Publikováno v:
PLoS ONE, Vol 17, Iss 11, p e0278151 (2022)
The spirochete Borrelia burgdorferi, which causes Lyme disease, has the most segmented genome among known bacteria. In addition to a linear chromosome, the B. burgdorferi genome contains over 20 linear and circular endogenous plasmids. While many of
Externí odkaz:
https://doaj.org/article/b6171b2af37a4cf993e7a13c9d7d44a0
Autor:
Danielle N Gallagher, Nhung Pham, Annie M Tsai, Nicolas V Janto, Jihyun Choi, Grzegorz Ira, James E Haber
Publikováno v:
PLoS Genetics, Vol 16, Iss 10, p e1008689 (2020)
The Rad51/RecA family of recombinases perform a critical function in typical repair of double-strand breaks (DSBs): strand invasion of a resected DSB end into a homologous double-stranded DNA (dsDNA) template sequence to initiate repair. However, rep
Externí odkaz:
https://doaj.org/article/69ec050854aa4461818f118bd5822bc8
Autor:
David P Waterman, Felix Zhou, Kevin Li, Cheng-Sheng Lee, Michael Tsabar, Vinay V Eapen, Allison Mazzella, James E Haber
Publikováno v:
PLoS Genetics, Vol 15, Iss 3, p e1008001 (2019)
We have used two different live-cell fluorescent protein markers to monitor the formation and localization of double-strand breaks (DSBs) in budding yeast. Using GFP derivatives of the Rad51 recombination protein or the Ddc2 checkpoint protein, we fi
Externí odkaz:
https://doaj.org/article/f7d1d2624d4b43669f8f1346f78bc08d
Publikováno v:
PLoS Genetics, Vol 14, Iss 5, p e1007424 (2018)
Mating-type switching in Schizosaccharomyces pombe entails programmed gene conversion events regulated by DNA replication, heterochromatin, and the HP1-like chromodomain protein Swi6. The whole mechanism remains to be fully understood. Using a gene d
Externí odkaz:
https://doaj.org/article/4ff933ff21ee44a7a5e2d2f41e590cb9
Publikováno v:
PLoS ONE, Vol 12, Iss 7, p e0180994 (2017)
Repair of a double-strand break (DSB) by an ectopic homologous donor sequence is subject to the three-dimensional arrangement of chromosomes in the nucleus of haploid budding yeast. The data for interchromosomal recombination suggest that searching f
Externí odkaz:
https://doaj.org/article/73171c1698ba4375bb222ea81f258865
Publikováno v:
PLoS Genetics, Vol 12, Iss 4, p e1005976 (2016)
Correct repair of DNA double-strand breaks (DSBs) is critical for maintaining genome stability. Whereas gene conversion (GC)-mediated repair is mostly error-free, repair by break-induced replication (BIR) is associated with non-reciprocal translocati
Externí odkaz:
https://doaj.org/article/05988166f6754c4cad8143e60a02c46d
Autor:
Michael Tsabar, Julian Haase, Benjamin Harrison, Chloe E Snider, Brittany Eldridge, Lila Kaminsky, Rebecca M Hine, James E Haber, Kerry Bloom
Publikováno v:
PLoS Genetics, Vol 12, Iss 4, p e1006021 (2016)
Transcriptional inactivation of the budding yeast centromere has been a widely used tool in studies of chromosome segregation and aneuploidy. In haploid cells when an essential chromosome contains a single conditionally inactivated centromere (GAL-CE
Externí odkaz:
https://doaj.org/article/9a31801e32dc4239b85f54a421ca94f8
Autor:
Matteo Ferrari, Diego Dibitetto, Giuseppe De Gregorio, Vinay V Eapen, Chetan C Rawal, Federico Lazzaro, Michael Tsabar, Federica Marini, James E Haber, Achille Pellicioli
Publikováno v:
PLoS Genetics, Vol 11, Iss 1, p e1004928 (2015)
The Mre11-Rad50-Xrs2 nuclease complex, together with Sae2, initiates the 5'-to-3' resection of Double-Strand DNA Breaks (DSBs). Extended 3' single stranded DNA filaments can be exposed from a DSB through the redundant activities of the Exo1 nuclease
Externí odkaz:
https://doaj.org/article/45c4e9bf900f4165a8718166d84934cd