Zobrazeno 1 - 10
of 14
pro vyhledávání: '"James D. Gwyer"'
Autor:
Vladimir Popov, Thomas G. Lowe, Tamara V. Tikhonova, Julea N. Butt, Sophie J. Marritt, James D. Gwyer, Myles R. Cheesman, Rose-Marie A. S. Doyle
Publikováno v:
JBIC Journal of Biological Inorganic Chemistry. 18:655-667
The multiheme cytochromes from Thioalkalivibrio nitratireducens (TvNiR) and Escherichia coli (EcNrfA) reduce nitrite to ammonium. Both enzymes contain His/His-ligated hemes to deliver electrons to their active sites, where a Lys-ligated heme has a di
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7f853473cd4edb9e93e2c1014996dd56
https://doi.org/10.1007/s00775-013-1011-7
https://doi.org/10.1007/s00775-013-1011-7
Publikováno v:
Analytical Chemistry. 84:1070-1075
This paper demonstrates an integrated microfluidic system that performs a full blood count using impedance analysis. A microfluidic network design for red blood cell (RBC) lysis is presented, and the diffusive mixing processes are analyzed using expe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9a3ee4505f2e45c81a91b42b4110306f
https://doi.org/10.1021/ac202700x
https://doi.org/10.1021/ac202700x
Autor:
Lars J. C. Jeuken, James K. R. Kendall, Phil Symonds, James D. Gwyer, Stephen D. Evans, Cees van Berkel, Benjamin R. G. Johnson, Guiseppi Imperato, Richard J. Bushby
Publikováno v:
ChemPhysChem. 11:2191-2198
Tethered bilayer lipid membranes (tBLM) are formed on 1) pure tether lipid triethyleneoxythiol cholesterol (EO(3)C) or on 2) mixed self-assembled monolayers (SAMs) of EO(3)C and 6-mercaptohexanol (6MH). While EO(3)C is required to form a tBLM with hi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ebc600cbc0f0839dd286b11d21066668
https://doi.org/10.1002/cphc.200900917
https://doi.org/10.1002/cphc.200900917
Publikováno v:
Biophysical Journal. 91(10):3897-3906
Escherichia coli cytochrome c nitrite reductase (NrfA) catalyzes the six-electron reduction of nitrite to perform an important role in the biogeochemical cycling of nitrogen. Here we describe NrfA adsorption on single-crystal Au(111) electrodes as an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::56e4a43a4502ff6776102d669d70647e
Publikováno v:
Biochemistry. 43:15086-15094
Cytochrome c nitrite reductase is a dimeric decaheme-containing enzyme that catalyzes the reduction of nitrite to ammonium. The contrasting effects of two inhibitors on the activity of this enzyme have been revealed, and defined, by protein film volt
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d9fe3c9250a316690d3931633a051626
https://doi.org/10.1021/bi049085x
https://doi.org/10.1021/bi049085x
Publikováno v:
Bioelectrochemistry. 63:43-47
Escherichia coli cytochrome c nitrite reductase is a homodimeric enzyme whose 10 heme centres range in reduction potential from ca. -30 to -320 mV. Protein film voltammetry (PFV) was performed to assess how the reactivity of the enzyme towards a numb
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::32180014e7d2a0150b0ea0e3dd92572e
https://doi.org/10.1016/j.bioelechem.2003.10.013
https://doi.org/10.1016/j.bioelechem.2003.10.013
Autor:
Cees, van Berkel, James D, Gwyer, Steve, Deane, Nicolas G, Green, Nicolas, Green, Judith, Holloway, Veronica, Hollis, Hywel, Morgan
Publikováno v:
Lab on a chip. 11(7)
Counting the different subpopulations of cells in a fingerprick of human blood is important for a number of clinical point-of-care (PoC) applications. It is a challenge to demonstrate the integration of sample preparation and detection techniques in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55dd3c7bbe821054346d05787c82574e
https://pubmed.ncbi.nlm.nih.gov/21331413
https://pubmed.ncbi.nlm.nih.gov/21331413
Autor:
James D. Gwyer, Thomas A. Clarke, B. Burlat, Myles R. Cheesman, Susannah R. Poock, J. A. Cole, Andrew M. Hemmings, David J. Richardson, Julea N. Butt
Publikováno v:
CIÊNCIAVITAE
ResearcherID
ResearcherID
The recent structural characterization of the NrfA from Escherichia coli provides a framework to rationalize the spectroscopic and functional properties of this enzyme. Analyses by EPR and magnetic CD spectroscopies have been complemented by protein-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8a85ecf9b69fd1923334418515c1abf4
https://pubmed.ncbi.nlm.nih.gov/15667286
https://pubmed.ncbi.nlm.nih.gov/15667286
Publikováno v:
Journal of the American Chemical Society. 127:14964-14965
Protein film voltammetry has been employed to define multiple catalytic consequences of proton coupled electron transfer (PCET) in a cytochrome c nitrite reductase. Current-potential profiles reflecting the steady-state rate of nitrite-limited reduct
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::16d1ae9beee7e26eeb66d3e4340e3505
https://doi.org/10.1021/ja054160s
https://doi.org/10.1021/ja054160s
Leukocyte analysis and differentiation using high speed microfluidic single cell impedance cytometry
Autor:
James D. Gwyer, Donna E. Davies, Christian H. Reccius, Hywel Morgan, David M. Pettigrew, Cees van Berkel, David Holmes, Judith A. Holloway
Publikováno v:
Lab on a Chip. 9:2881
Miniature high speed label-free cell analysis systems have yet to be developed, but have the potential to deliver fast, inexpensive and simple full blood cell analysis systems that could be used routinely in clinical practice. We demonstrate a microf
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::130836b94839827d88b5c49c2bfd8ed7
https://doi.org/10.1039/b910053a
https://doi.org/10.1039/b910053a