Post-transcriptional regulatory feedback encodes JAK-STAT signal memory of interferon stimulation

Autor: Eirini Kalliara, Malgorzata Kardynska, James Bagnall, David G. Spiller, Werner Müller, Dominik Ruckerl, Jarosław Śmieja, Subhra K. Biswas, Pawel Paszek

Publikováno v: Frontiers in Immunology, Vol 13 (2022)

Immune cells fine tune their responses to infection and inflammatory cues. Here, using live-cell confocal microscopy and mathematical modelling, we investigate interferon-induced JAK-STAT signalling in innate immune macrophages. We demonstrate that t

Externí odkaz: https://doaj.org/article/f18214bc8b124365b6050d91ca9f4e00

Quantitative live imaging of Venus::BMAL1 in a mouse model reveals complex dynamics of the master circadian clock regulator.

Autor: Nan Yang, Nicola J Smyllie, Honor Morris, Cátia F Gonçalves, Michal Dudek, Dharshika R J Pathiranage, Johanna E Chesham, Antony Adamson, David G Spiller, Egor Zindy, James Bagnall, Neil Humphreys, Judith Hoyland, Andrew S I Loudon, Michael H Hastings, Qing-Jun Meng

Publikováno v: PLoS Genetics, Vol 16, Iss 4, p e1008729 (2020)

Evolutionarily conserved circadian clocks generate 24-hour rhythms in physiology and behaviour that adapt organisms to their daily and seasonal environments. In mammals, the suprachiasmatic nucleus (SCN) of the hypothalamus is the principal co-ordina

Externí odkaz: https://doaj.org/article/43fa25b09c644bd38b2e46c67b3637c5

Signal transduction controls heterogeneous NF-κB dynamics and target gene expression through cytokine-specific refractory states

Autor: Antony Adamson, Christopher Boddington, Polly Downton, William Rowe, James Bagnall, Connie Lam, Apolinar Maya-Mendoza, Lorraine Schmidt, Claire V. Harper, David G. Spiller, David A. Rand, Dean A. Jackson, Michael R. H. White, Pawel Paszek

Publikováno v: Nature Communications, Vol 7, Iss 1, Pp 1-14 (2016)

In biological systems, timing is often critical to the interpretation of signals that determine cell fate. Here the authors demonstrate how single cells and cellular populations respond dynamically to pulsatile stimulation by TNFα and IL-1β, and su

Externí odkaz: https://doaj.org/article/1d4f418033bb45d7b771ae8b6ba8118e

Quantitative Characterisation of Low Abundant Yeast Mitochondrial Proteins Reveals Compensation for Haplo-Insufficiency in Different Environments

Autor: Alkisti Manousaki, James Bagnall, David Spiller, Laura Natalia Balarezo-Cisneros, Michael White, Daniela Delneri

Publikováno v: International Journal of Molecular Sciences; Volume 23; Issue 15; Pages: 8532

Quantification of low abundant membrane-binding proteins such as transcriptional factors and chaperones has been proved difficult even with the most sophisticated analytical technologies. Here we exploit and optimise the non-invasive Fluorescence Cor

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::985d94212015b244cf792f513149059a

The clock gene Bmal1 inhibits macrophage motility, phagocytosis, and impairs defense against pneumonia

Autor: John Blaikley, Gareth B. Kitchen, Tracy Hussell, Mudassar Iqbal, Robert Maidstone, Laura Matthews, Hannah J. Durrington, Peter S. Cunningham, James Bagnall, Nicola Begley, Matthew Baxter, Ben Saer, Toryn Poolman, Julie E. Gibbs, Andrew S. I. Loudon, David W. Ray, David H. Dockrell

Publikováno v: Kitchen, G B, Cunningham, P S, Poolman, T M, Iqbal, M, Maidstone, R, Baxter, M, Bagnall, J, Begley, N, Saer, B, Hussell, T, Matthews, L C, Dockrell, D H, Durrington, H J, Gibbs, J E, Blaikley, J F, Loudon, A S & Ray, D W 2020, ' The clock gene Bmal1 inhibits macrophage motility, phagocytosis, and impairs defense against pneumonia ', Proceedings of the National Academy of Sciences, vol. 117, no. 3, pp. 1543-1551 . https://doi.org/10.1073/pnas.1915932117

The circadian clock regulates many aspects of immunity. Bacterial infections are affected by time of day, but the mechanisms involved remain undefined. Here we show that loss of the core clock protein BMAL1 in macrophages confers protection against p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0f2f3ec07d41a024bb148b8e2b4eb0d7
https://doi.org/10.1073/pnas.1915932117