Zobrazeno 1 - 10
of 11
pro vyhledávání: '"James B, McGivney"'
Autor:
Liangliang Sun, Norman J. Dovichi, Ronald Schoner, Thomas Albanetti, Guijie Zhu, James B. McGivney, Travis Linkous, Christopher Larkin
Publikováno v:
Biotechnology and Bioengineering. 113:2140-2148
We employed UPLC-MS/MS with iTRAQ 8-plex labeling to quantitatively analyze the supernatant produced by two Chinese hamster ovary (CHO) cell lines (CHO K1SV and CHO CAT-S). In each case, the supernatant from the host and three transfected clones were
Autor:
Christopher Larkin, Santosh Kumar Paidi, James B. McGivney, Ishan Barman, Soumik Siddhanta, Robert Strouse
Publikováno v:
Analytical Chemistry. 88:4361-4368
Product identification is a critical and required analysis for biotheraputics. In addition to regulatory requirements for identity testing on final drug products, in-process identity testing is implemented to reduce business risks associated with fil
Autor:
Srilatha Kuntumalla, Guijie Zhu, Hung Yu Lin, James B. McGivney, Christopher Larkin, Norman J. Dovichi, Jennifer Heidbrink-Thompson, Liangliang Sun
Publikováno v:
ELECTROPHORESIS. 37:616-622
We have evaluated CZE-ESI-MS/MS for detection of trace amounts of host cell protein impurities in recombinant therapeutics. Compared to previously published procedures, we have optimized the buffer pH used in the formation of a pH junction to increas
Autor:
Peter L. Mage, H. Tom Soh, Robert Strouse, James B. McGivney, Elizabeth R. Bagley, Michael Eisenstein, Faye M. Walker, Andrew T. Csordas, Margaret A. Nakamoto, Jose Casas-Finet, Christopher Larkin, Emily M. Gruber
Publikováno v:
Analytical chemistry. 90(5)
Biologic drugs are typically manufactured in mammalian host cells, and it is critical from a drug safety and efficacy perspective to detect and remove host cell proteins (HCPs) during production. This is currently achieved with sets of polyclonal ant
Autor:
Dawn Kernaghan, Guijie Zhu, James B. McGivney, Travis Linkous, Liangliang Sun, Norman J. Dovichi
Publikováno v:
ELECTROPHORESIS. 35:1448-1452
We report the first use of CZE for absolute characterization of host cell proteins (HCPs) in recombinant human monoclonal antibodies. An electrokinetically pumped nanoelectrospray interface was used to couple CZE with a tandem mass spectrometer. Thre
Autor:
Travis Linkous, Thomas Albanetti, Norman J. Dovichi, Ronald Schoner, James B. McGivney, Christopher Larkin, Zhenbin Zhang
Publikováno v:
Electrophoresis. 38(3-4)
Four methods were compared for analysis of host-cell protein (HCP) impurities in a recombinant mAb. First, CZE-MS/MS was used to analyze the digest of an HCP sample following extraction of the mAb with proteins A and L affinity columns; 220 protein g
Autor:
Norman J. Dovichi, Roza Wojcik, Dawn Kernaghan, Guijie Zhu, James B. McGivney, Liangliang Sun
Publikováno v:
Talanta. 98:253-256
A rapid and reproducible system that couples capillary isoelectric focusing to a high-resolution mass spectrometer was developed for on-line analysis and identification of protein digests. Magnetic microsphere-based immobilized trypsin was used for p
Autor:
Mark A. Schenerman, Chris D. Geddes, Robert Strouse, Anatoliy I. Dragan, Jose Casas-Finet, James B. McGivney, E.S. Bishop
Publikováno v:
Plasmonics. 7:739-744
In recent years both the mechanism and applications of metal-enhanced fluorescence (MEF) have attracted significant attention, yet many fundamental aspects of MEF remain unanswered or addressed. In this study, we address a fundamental aspect of MEF.
Autor:
Guijie, Zhu, Liangliang, Sun, Thomas, Albanetti, Travis, Linkous, Christopher, Larkin, Ronald, Schoner, James B, McGivney, Norman J, Dovichi
Publikováno v:
Biotechnology and bioengineering. 113(10)
We employed UPLC-MS/MS with iTRAQ 8-plex labeling to quantitatively analyze the supernatant produced by two Chinese hamster ovary (CHO) cell lines (CHO K1SV and CHO CAT-S). In each case, the supernatant from the host and three transfected clones were
Autor:
Guijie, Zhu, Liangliang, Sun, Travis, Linkous, Dawn, Kernaghan, James B, McGivney, Norman J, Dovichi
Publikováno v:
Electrophoresis. 35(10)
We report the first use of CZE for absolute characterization of host cell proteins (HCPs) in recombinant human monoclonal antibodies. An electrokinetically pumped nanoelectrospray interface was used to couple CZE with a tandem mass spectrometer. Thre