Zobrazeno 1 - 10
of 23
pro vyhledávání: '"James A. Sawitzke"'
Assembly of intact, replicating plasmids from linear DNA fragments introduced into bacterial cells, i.e. in vivo cloning, is a facile genetic engineering technology that avoids many of the problems associated with standard in vitro cloning. Here we r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::546df2f3666cdda137c4039f25bd82a5
https://doi.org/10.1101/2022.01.13.476211
https://doi.org/10.1101/2022.01.13.476211
Autor:
Daniel P Haeusser, Marina Hoashi, Anna Weaver, Nathan Brown, James Pan, James A Sawitzke, Lynn C Thomason, Donald L Court, William Margolin
Publikováno v:
PLoS Genetics, Vol 10, Iss 3, p e1004217 (2014)
Assembly of the essential, tubulin-like FtsZ protein into a ring-shaped structure at the nascent division site determines the timing and position of cytokinesis in most bacteria and serves as a scaffold for recruitment of the cell division machinery.
Externí odkaz:
https://doaj.org/article/ae8e842a32994f1fb68ed4dce7b959ed
Autor:
Sandra K. Weller, James A. Sawitzke
Publikováno v:
Annual Review of Microbiology. 68:237-258
The purpose of this review is to explore recombination strategies in DNA viruses. Homologous recombination is a universal genetic process that plays multiple roles in the biology of all organisms, including viruses. Recombination and DNA replication
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5fbb4afc1301928d16fafd5bb717aa4f
https://doi.org/10.1146/annurev-micro-091313-103424
https://doi.org/10.1146/annurev-micro-091313-103424
Publikováno v:
Molecular Microbiology. 88:906-920
Synthetic single-strand oligonucleotides (oligos) with homology to genomic DNA have proved to be highly effective for constructing designed mutations in targeted genomes, a process referred to as recombineering. The cellular functions important for t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ddef06b6950ddf9686634ad3da655f66
https://doi.org/10.1111/mmi.12231
https://doi.org/10.1111/mmi.12231
Autor:
Donald L. Court, James A. Sawitzke, Teresa Baker, Helen R. Wilson, Mikail Bubunenko, Shyam K. Sharan, Sundaram Swaminathan, Daiguan Yu
Publikováno v:
Gene. 315:63-69
The bacteriophage lambda (lambda) recombination system Red has been used for engineering large DNA fragments cloned into P1 and bacterial artificial chromosomes (BAC or PAC) vectors. So far, this recombination system has been utilized by transferring
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::028b274ff9b7743c333789359d08dbc5
https://doi.org/10.1016/s0378-1119(03)00728-5
https://doi.org/10.1016/s0378-1119(03)00728-5
Publikováno v:
Proceedings of the National Academy of Sciences. 100:7207-7212
A phage λ-based recombination system, Red, can be used for high-efficiency mutagenesis, repair, and engineering of chromosomal or episomal DNA in vivo in Escherichia coli . When long linear double-stranded DNA with short flanking homologies to their
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::131ea143040ff0644e59c1654e8380ce
https://doi.org/10.1073/pnas.1232375100
https://doi.org/10.1073/pnas.1232375100
Autor:
Mikhail Bubunenko, Amos B. Oppenheim, Simanti Datta, Donald L. Court, Lynn C. Thomason, Richard S. Myers, Nina Costantino, James A. Sawitzke
This chapter describes the present state of recombineering in Escherichia coli and details the essential elements of the system. The success of this technology has encouraged its development for use in other organisms. The chapter discusses that goal
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0ddf51e89245f6ecffaa2d945007f357
https://doi.org/10.1128/9781555816506.ch19
https://doi.org/10.1128/9781555816506.ch19
Autor:
James A. Sawitzke
Publikováno v:
The Lure ofBacterial Genetics ISBN: 9781683671237
The Lure ofBacterial Genetics
The Lure ofBacterial Genetics
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8075c21fe0549619cf1731d77d7cff18
https://doi.org/10.1128/9781555816810.ch32
https://doi.org/10.1128/9781555816810.ch32
Autor:
James A. Sawitzke, Stuart Austin
Publikováno v:
Molecular Microbiology. 40:786-794
Recent advances in microscopy have given us important clues as to the nature of chromosome segregation in bacteria. Most current observations favour the view that the process is co-replicational: DNA replication forks are anchored at the cell centre,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01700bbeabf996a9d3987afe3e7f934d
https://doi.org/10.1046/j.1365-2958.2001.02350.x
https://doi.org/10.1046/j.1365-2958.2001.02350.x
Autor:
James A. Sawitzke, Stuart Austin
Publikováno v:
Proceedings of the National Academy of Sciences. 97:1671-1676
Escherichia coli muk mutants are temperature-sensitive and produce anucleate cells. A spontaneously occurring mutation was found in a ΔmukB∷kan mutant strain that suppressed the temperature-sensitive phenotype and mapped in or near topA , the gene
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce60c9e68010171012e28b26541bdcb0
https://doi.org/10.1073/pnas.030528397
https://doi.org/10.1073/pnas.030528397