Zobrazeno 1 - 10
of 49
pro vyhledávání: '"James A. Parkhurst"'
Autor:
James M. Parkhurst, Adam D. Crawshaw, C. Alistair Siebert, Maud Dumoux, C. David Owen, Pedro Nunes, David Waterman, Thomas Glen, David I. Stuart, James H. Naismith, Gwyndaf Evans
Publikováno v:
IUCrJ, Vol 10, Iss 3, Pp 270-287 (2023)
Three-dimensional electron diffraction (3DED) from nanocrystals of biological macromolecules requires the use of very small crystals. These are typically less than 300 nm-thick in the direction of the electron beam due to the strong interaction betwe
Externí odkaz:
https://doaj.org/article/e0f2750b21824a2da2eac4a6e181c76f
Autor:
Melanie Vollmar, James M. Parkhurst, Dominic Jaques, Arnaud Baslé, Garib N. Murshudov, David G. Waterman, Gwyndaf Evans
Publikováno v:
IUCrJ, Vol 7, Iss 2, Pp 342-354 (2020)
This study describes a method to estimate the likelihood of success in determining a macromolecular structure by X-ray crystallography and experimental single-wavelength anomalous dispersion (SAD) or multiple-wavelength anomalous dispersion (MAD) pha
Externí odkaz:
https://doaj.org/article/ddfba1d3f2eb453ca6f10a0722b1cf77
Autor:
James M. Parkhurst, Maud Dumoux, Mark Basham, Daniel Clare, C. Alistair Siebert, Trond Varslot, Angus Kirkland, James H. Naismith, Gwyndaf Evans
Publikováno v:
Open Biology, Vol 11, Iss 10 (2021)
In cryo-electron tomography (cryo-ET) of biological samples, the quality of tomographic reconstructions can vary depending on the transmission electron microscope (TEM) instrument and data acquisition parameters. In this paper, we present Parakeet, a
Externí odkaz:
https://doaj.org/article/70cf5223a322452fb78cf19561387825
Autor:
Emma V. Beale, David G. Waterman, Corey Hecksel, Jason van Rooyen, James B. Gilchrist, James M. Parkhurst, Felix de Haas, Bart Buijsse, Gwyndaf Evans, Peijun Zhang
Publikováno v:
Frontiers in Molecular Biosciences, Vol 7 (2020)
MicroED has recently emerged as a powerful method for the analysis of biological structures at atomic resolution. This technique has been largely limited to protein nanocrystals which grow either as needles or plates measuring only a few hundred nano
Externí odkaz:
https://doaj.org/article/a360773de2214620ac65ecc967425cdd
Autor:
Patrick Phillips, James M. Parkhurst, Ilias Kounatidis, Chidinma Okolo, Thomas M. Fish, James H. Naismith, Martin A. Walsh, Maria Harkiolaki, Maud Dumoux
Publikováno v:
Life, Vol 11, Iss 8, p 842 (2021)
Chlamydiae are strict intracellular pathogens residing within a specialised membrane-bound compartment called the inclusion. Therefore, each infected cell can, be considered as a single entity where bacteria form a community within the inclusion. It
Externí odkaz:
https://doaj.org/article/64f44ae1b4934a729c27ba86c7e78f61
Autor:
James M. Parkhurst, Andrea Thorn, Melanie Vollmar, Graeme Winter, David G. Waterman, Luis Fuentes-Montero, Richard J. Gildea, Garib N. Murshudov, Gwyndaf Evans
Publikováno v:
IUCrJ, Vol 4, Iss 5, Pp 626-638 (2017)
An algorithm for modelling the background for each Bragg reflection in a series of X-ray diffraction images containing Debye–Scherrer diffraction from ice in the sample is presented. The method involves the use of a global background model which is
Externí odkaz:
https://doaj.org/article/d34cbd0f1e4e45eeb904b3c52112dd06
Autor:
David G. Waterman, Melanie Vollmar, Dominic Jaques, Garib N. Murshudov, Gwyndaf Evans, Arnaud Baslé, James M. Parkhurst
Publikováno v:
IUCrJ
IUCrJ, Vol 7, Iss 2, Pp 342-354 (2020)
IUCrJ, Vol 7, Iss 2, Pp 342-354 (2020)
Combining data-analysis statistics from crystallographic software with machine learning can predict the chances of experimental phasing success.
This study describes a method to estimate the likelihood of success in determining a macromolecular
This study describes a method to estimate the likelihood of success in determining a macromolecular
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e3404927a6756175a0b4d6fa85b3fbeb
http://europepmc.org/articles/PMC7055369
http://europepmc.org/articles/PMC7055369
Autor:
Mark Basham, A Kirkland, Daniel K. Clare, Maud Dumoux, James H. Naismith, T Varslot, Gwyndaf Evans, C A Siebert, James M. Parkhurst
Publikováno v:
Open Biology
Open Biology, Vol 11, Iss 10 (2021)
Open Biology, Vol 11, Iss 10 (2021)
In cryo-electron tomography (cryo-ET) of biological samples, the quality of tomographic reconstructions can vary depending on the transmission electron microscope (TEM) instrument and data acquisition parameters. In this paper, we present Parakeet, a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a6c81a48c599be575d2fbd39532e6955
https://pubmed.ncbi.nlm.nih.gov/34699732
https://pubmed.ncbi.nlm.nih.gov/34699732
Autor:
Ilias Kounatidis, Chidinma A. Okolo, James H. Naismith, Thomas M. Fish, Maria Harkiolaki, Martin A. Walsh, Patrick Phillips, James M. Parkhurst, Maud Dumoux
Publikováno v:
Life, Vol 11, Iss 842, p 842 (2021)
Life; Volume 11; Issue 8; Pages: 842
Life; Volume 11; Issue 8; Pages: 842
The impact of the cell community on its individual components in the prokaryotic realm is often overlooked. However, in the case of intracellular pathogens, where each infected cell can be considered as a single community, understanding how a populat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f9824ee62cc7193acde16fbf9158480
Publikováno v:
Acta Crystallographica. Section D, Structural Biology
Adaptations to the DIALS package are described that make it a suitable choice for processing challenging continuous-rotation electron diffraction data. The results of using the extended package are presented for a case consisting of seven example dat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf1866a4962a15f0ec4a09b1bdf0625b
http://europepmc.org/articles/PMC6096487
http://europepmc.org/articles/PMC6096487